HIV-1 Replication Benefits from the RNA Epitranscriptomic Code.

abstract：:Ribosomal subunit joining is a key checkpoint in the bacterial translation initiation pathway during which initiation factors (IFs) regulate association of the 30S initiation complex (IC) with the 50S subunit to control formation of a 70S IC that can enter into the elongation stage of protein synthesis. The GTP-bound ...

journal_title：Journal of molecular biology

authors： MacDougall DD,Gonzalez RL Jr

更新日期：2015-05-08 00:00:00

abstract：:The minichromosome maintenance (MCM) proteins are essential for replication initiation and elongation in eukarya and archaea. There are six MCM proteins in eukaryotes, and MCM complexes are believed to unwind DNA during chromosomal DNA replication. However, the mechanism and structure of the MCM complexes are not know...

journal_title：Journal of molecular biology

authors： Chen YJ,Yu X,Kasiviswanathan R,Shin JH,Kelman Z,Egelman EH

更新日期：2005-02-18 00:00:00

abstract：:Cytosolic 5'-nucleotidase III (cN-III) is responsible for selective degradation of pyrimidine 5'-monoribonucleotides during maturation of reticulocytes to erythrocytes. The lack of this enzymatic activity due to genetic aberrations or lead poisoning results in a mild to moderate nonspherocytic hemolytic anemia. In aff...

journal_title：Journal of molecular biology

authors： Grobosky CL,Lopez JB,Rennie S,Skopelitis DJ,Wiest AT,Bingman CA,Bitto E

更新日期：2012-11-02 00:00:00

abstract：:We present the first large-scale survey of N-terminal protein maturation in archaea based on 873 proteomically identified N-terminal peptides from the two haloarchaea Halobacterium salinarum and Natronomonas pharaonis. The observed protein maturation pattern can be attributed to the combined action of methionine amino...

journal_title：Journal of molecular biology

authors： Falb M,Aivaliotis M,Garcia-Rizo C,Bisle B,Tebbe A,Klein C,Konstantinidis K,Siedler F,Pfeiffer F,Oesterhelt D

更新日期：2006-10-06 00:00:00

abstract：:The resonances of all the base protons and most of the sugar protons in both strands of the 17 base-pair OR3 operator of the phage lambda, and of the vC3 single base-pair mutant, have been assigned using two-dimensional nuclear magnetic resonance methods. The chemical shift and nuclear Overhauser effect data for these...

journal_title：Journal of molecular biology

authors： Wemmer DE,Chou SH,Reid BR

更新日期：1984-11-25 00:00:00

abstract：:The induction of effector genes that encode enzymes is often controlled by the protein product of a regulator gene that is directly involved in the control of its own expression. This coupling of elementary gene circuits can lead to three patterns of regulator and effector gene expression. As effector gene expression ...

journal_title：Journal of molecular biology

authors： Hlavacek WS,Savageau MA

更新日期：1996-01-12 00:00:00

abstract：:The nuclear receptor pregnane X receptor (PXR) acts as a sensor for a broad variety of natural and synthetic lipophilic compounds, such as bile acids and rifampicin, and regulates the expression of proteins that are involved in the metabolism and transport of these compounds. PXR binds as a heterodimer with the retino...

journal_title：Journal of molecular biology

authors： Frank C,Makkonen H,Dunlop TW,Matilainen M,Väisänen S,Carlberg C

更新日期：2005-02-18 00:00:00

abstract：:Interferons (IFN) are multi-functional proteins that induce a large number of genes which mediate many biological processes including host defense, cell growth control, signaling, and metabolism. Bioinformatics analysis of the 3'-untranslated regions of IFN-stimulated genes (ISGs) showed that the AU-rich elements (ARE...

journal_title：Journal of molecular biology

authors： Khabar KS,Al-Haj L,Al-Zoghaibi F,Marie M,Dhalla M,Polyak SJ,Williams BR

更新日期：2004-09-17 00:00:00

abstract：:We report studies to determine which bacteriophage genes are required for assembly of phage HK97 proheads and what roles they play. We identify the gene encoding the major capsid protein of phage HK97 and report its DNA sequence, together with the DNA sequences of the two genes immediately upstream from it. When the c...

journal_title：Journal of molecular biology

authors： Duda RL,Martincic K,Hendrix RW

更新日期：1995-04-07 00:00:00

abstract：:We use flexible backbone protein design to explore the sequence and structure neighborhoods of naturally occurring proteins. The method samples sequence and structure space in the vicinity of a known sequence and structure by alternately optimizing the sequence for a fixed protein backbone using rotamer based sequence...

journal_title：Journal of molecular biology

authors： Saunders CT,Baker D

更新日期：2005-02-18 00:00:00

abstract：:An accurate three-dimensional structure is known for papain (1.65 A resolution) and actinidin (1.7 A). A detailed comparison of these two structures was performed to determine the effect of amino acid changes on the conformation. It appeared that, despite only 48% identity in their amino acid sequence, different cryst...

journal_title：Journal of molecular biology

authors： Kamphuis IG,Drenth J,Baker EN

更新日期：1985-03-20 00:00:00

abstract：:X-ray quality single crystals of an extracellular esterase from pathogenic Streptomyces scabies were obtained by the hanging drop method. The crystals are monoclinic (space group C2, a = 161.1 A, b = 51.2 A, c = 124.2 A, beta = 100.6 degrees) with two molecules related by a noncrystallographic dyad in the asymmetric u...

journal_title：Journal of molecular biology

authors： Green R,Schottel JL,Swenson L,Wei Y,Derewenda ZS

更新日期：1992-09-20 00:00:00

abstract：:Stochastic fluctuations, termed "noise," in the level of biological molecules can greatly impact cellular functions. While biological noise can sometimes be detrimental, recent studies have provided an increasing number of examples in which biological noise can be functionally beneficial. Rather than provide an exhaus...

journal_title：Journal of molecular biology

authors： Harton MD,Batchelor E

更新日期：2017-04-21 00:00:00

abstract：:The nucleotide sequence was obtained for the hypE gene in the cluster of structural and accessory genes required for the assembly and functioning of the membrane-bound, dimeric, (NiFe)hydrogenase in Azotobacter vinelandii. The hypE gene encodes a polypeptide of 341 amino acid residues which is rich in alanine, glycine...

journal_title：Journal of molecular biology

authors： Garg RP,Menon AL,Jacobs K,Robson RM,Robson RL

更新日期：1994-02-11 00:00:00

abstract：:An essential step of pre-mRNA spliceosome assembly is the interaction between the snRNPs U4/U6 and U5, to form the [U4/U6.U5] tri-snRNP. While the tri-snRNP protein Prp6p appears to play an important role for tri-snRNP formation in yeast, little is known about the interactions that connect the two snRNP particles in h...

journal_title：Journal of molecular biology

authors： Makarov EM,Makarova OV,Achsel T,Lührmann R

更新日期：2000-05-12 00:00:00

abstract：:Protein allostery plays a pivotal role in many regulatory processes. Prominent examples are cell-surface receptors, which allosterically transmit ligand-generated signals to their cytoplasmic domains, or bacterial transcription factors, which alternate between a free conformation and a DNA-bound conformation in respon...

journal_title：Journal of molecular biology

authors： Goeke D,Kaspar D,Stoeckle C,Grubmüller S,Berens C,Klotzsche M,Hillen W

更新日期：2012-02-10 00:00:00

abstract：:Escherichia coli induces the synthesis of at least 30 proteins at the onset of carbon starvation, two-thirds of which are positively regulated by the cyclic AMP (cAMP) and cAMP receptor protein (CRP) complex. Two of the cAMP-CRP-dependent genes mapped to 14 and 93 minutes of the chromosome and are designated cstA and ...

journal_title：Journal of molecular biology

authors： Schultz JE,Matin A

更新日期：1991-03-05 00:00:00

abstract：:The Mycobacterium leprae RuvA homologue (MlRuvA) was over-expressed in Escherichia coli and purified to homogeneity. The DNA-binding specificity and the functional interactions of MlRuvA with E. coli RuvB and RuvC (EcRuvB and EcRuvC) were examined using synthetic Holliday junctions. MlRuvA bound specifically to Hollid...

journal_title：Journal of molecular biology

authors： Arenas-Licea J,van Gool AJ,Keeley AJ,Davies A,West SC,Tsaneva IR

更新日期：2000-08-25 00:00:00

abstract：:Three-dimensional domain swapping has emerged as a ubiquitous process for homo-oligomer formation in many unrelated proteins, but the molecular mechanism of this process is still poorly understood. Here we present a mechanism for the swapping reaction in the B1 domain of the immunoglobulin G binding protein from group...

journal_title：Journal of molecular biology

authors： Malevanets A,Sirota FL,Wodak SJ

更新日期：2008-09-26 00:00:00

abstract：:The growth of one group of amber mutants of R17 (class A) in non-permissive hosts results in a burst of bacteriophage-like particles, which are defective in that they are non-infectious. Here studies of particles produced by the growth of wild-type R17 and four class A mutants on AB301, a non-permissive Hfr Escherichi...

journal_title：Journal of molecular biology

authors： Argetsinger JE,Gussin GN

更新日期：1966-11-28 00:00:00

abstract：:Kinetic models were developed to describe the influence of prolyl peptide bond isomerization on the kinetics of reversible protein folding for cases in which structural intermediates do not occur. In the simulations, the number of prolyl residues and the relative rates of folding and isomerization were varied. The exp...

journal_title：Journal of molecular biology

authors： Kiefhaber T,Kohler HH,Schmid FX

更新日期：1992-03-05 00:00:00

abstract：:Thioredoxins are low-molecular-mass proteins that function as hydrogen carriers in DNA synthesis and in the transformation of sulfur metabolites. They also act as regulatory proteins in the light-dependent enzyme activation during photosynthesis. F-type thioredoxin from spinach chloroplasts, a monomeric protein of 113...

journal_title：Journal of molecular biology

authors： Génovésio-Taverne JC,Jetzer Y,Sauder U,Hohenester E,Hughet C,Jansonius JN,Gardet-Salvi L,Schürmann P

更新日期：1991-12-05 00:00:00

abstract：:The crystal structure of muconate lactonizing enzyme has been solved at 3 A resolution, and an unambiguous alpha-carbon backbone chain trace made. The enzyme contains three domains; the central domain is a parallel-stranded alpha-beta barrel, which has previously been reported in six other enzymes, including triose ph...

journal_title：Journal of molecular biology

authors： Goldman A,Ollis DL,Steitz TA

更新日期：1987-03-05 00:00:00

abstract：:The genes coding for translation initiation factor IF3 (infC) and for the ribosomal proteins L35 (rpmI) and L20 (rplT) are transcribed in that order from a promoter in front of infC. The last two cistrons of the operon (rpmI and rplT) can be transcribed from a weak secondary promoter situated within the first cistron ...

journal_title：Journal of molecular biology

authors： Lesage P,Truong HN,Graffe M,Dondon J,Springer M

更新日期：1990-06-05 00:00:00

abstract：:The formation of membraneless organelles (MLOs) by phase separation has emerged as a new way of organizing the cytoplasm and nucleoplasm of cells. Examples of MLOs forming via phase separation are nucleoli in the nucleus and stress granules in the cytoplasm. The main components of these MLOs are macromolecules such as...

journal_title：Journal of molecular biology

authors： Alberti S,Carra S

更新日期：2018-11-02 00:00:00

abstract：:Staphylococcus aureus A70 produces a heat-stable bacteriocin designated aureocin A70. Aureocin A70 is encoded within a mobilisable 8 kb plasmid, pRJ6, and is active against Listeria monocytogenes. Experiments of transposition mutagenesis and gene cloning had shown that aureocin A70 production and immunity were associa...

journal_title：Journal of molecular biology

authors： Netz DJ,Sahl HG,Marcelino R,dos Santos Nascimento J,de Oliveira SS,Soares MB,do Carmo de Freire Bastos M

更新日期：2001-08-31 00:00:00

abstract：:Crystal structures of Bacillus stearothermophilus TRS40 neopullulanase and its complexes with panose, maltotetraose and isopanose were determined at resolutions of 1.9, 2.4, 2.8 and 3.2A, respectively. Since the latter two carbohydrates are substrates of this enzyme, a deactivated mutant at the catalytic residue Glu35...

journal_title：Journal of molecular biology

authors： Hondoh H,Kuriki T,Matsuura Y

更新日期：2003-02-07 00:00:00

abstract：:An open reading frame optimized for expression of 6,7-dimethyl-8-ribityl-lumazine synthase of the hyperthermophilic bacterium Aquifex aeolicus in Escherichia coli was synthesized and expressed in a recombinant E. coli strain to a level of around 15 %. The recombinant protein was purified by heat-treatment and gel-filt...

journal_title：Journal of molecular biology

authors： Zhang X,Meining W,Fischer M,Bacher A,Ladenstein R

更新日期：2001-03-09 00:00:00

abstract：:We propose a general classification scheme for loops, aperiodic segments of protein structure. In an effort to avoid the geometric complexity created by non-repeating phi psi angles, a morphologic definition that focuses upon the linearity and planarity of loops is utilized. Out of 432 loops (4 to 20 residues in lengt...

journal_title：Journal of molecular biology

authors： Ring CS,Kneller DG,Langridge R,Cohen FE

更新日期：1992-04-05 00:00:00

abstract：:We show that the three core histones H2A, H3 and H4 can transverse lipid bilayers of large unilamellar vesicles (LUVs) and multilamellar vesicles (MLVs). In contrast, the histone H2B, although able to bind to the liposomes, fails to penetrate the unilamellar and the multilamellar vesicles. Translocation across the lip...

journal_title：Journal of molecular biology

authors： Rosenbluh J,Hariton-Gazal E,Dagan A,Rottem S,Graessmann A,Loyter A

更新日期：2005-01-14 00:00:00