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Crystallization and preliminary crystallographic data of a Streptomyces scabies extracellular esterase.

Abstract：

:X-ray quality single crystals of an extracellular esterase from pathogenic Streptomyces scabies were obtained by the hanging drop method. The crystals are monoclinic (space group C2, a = 161.1 A, b = 51.2 A, c = 124.2 A, beta = 100.6 degrees) with two molecules related by a noncrystallographic dyad in the asymmetric unit, with a solvent content of approximately 64%. The diffraction pattern from fresh crystals extends beyond 2 A resolution using sealed tube CuK alpha radiation. The study has been initiated in order to elucidate the mechanism of this unusual non-serine-dependent esterase, and to gain better understanding of the molecular basis of the pathogenesis of the scab disease.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Green R,Schottel JL,Swenson L,Wei Y,Derewenda ZS

doi

10.1016/0022-2836(92)90908-3

keywords:

subject

Has Abstract

pub_date

1992-09-20 00:00:00

pages

569-71

issue

2

eissn

0022-2836

issn

1089-8638

pii

0022-2836(92)90908-3

journal_volume

227

pub_type

杂志文章

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