Cell Heterogeneity in Staphylococcal Communities.

abstract：:DNA structures bound to transcription factors are studied by using the crystal coordinates of complexes. Structural characteristics which are found at the sites where a protein secondary structure, an alpha-helix or a beta-sheet binds, can be understood in terms of fitting of the concave surface of the DNA major groov...

journal_title：Journal of molecular biology

authors： Suzuki M,Yagi N

更新日期：1996-02-09 00:00:00

abstract：:Junonia coenia densovirus (JcDNV) belongs to the densovirus genus of the Parvoviridae family and infects the larvae of the Common Buckeye butterfly. Its capsid is icosahedral and consists of viral proteins VP1 (88 kDa), VP2 (58 kDa), VP3 (52 kDa) and VP4 (47 kDa). Each viral protein has the same C terminus but differs...

journal_title：Journal of molecular biology

authors： Bruemmer A,Scholari F,Lopez-Ferber M,Conway JF,Hewat EA

更新日期：2005-04-08 00:00:00

abstract：:Apolipoprotein A-I (ApoA-I) is a protein implicated in the solubilization of lipids and cholesterol from cellular membranes. The study of ApoA-I in phospholipid (PL) monolayers brings relevant information about ApoA-I/PL interactions. We investigated the influence of PL charge and acyl chain organization on the intera...

journal_title：Journal of molecular biology

authors： Chièze L,Bolanos-Garcia VM,Pinot M,Desbat B,Renault A,Beaufils S,Vié V

更新日期：2011-07-01 00:00:00

abstract：:Thermodynamic analysis by differential scanning calorimetry shows that the folding/unfolding transition of staphylococcal nuclease is consistent with the two-state process. Stopped-flow kinetic measurements, monitoring the Trp140 fluorescence and covering five decades in time (2 ms to 300 s), indicate that the unfoldi...

journal_title：Journal of molecular biology

authors： Chen HM,You JL,Markin VS,Tsong TY

更新日期：1991-08-05 00:00:00

abstract：:The overexpression of authentically folded eukaryotic membrane proteins in milligramme quantities is a fundamental prerequisite for structural studies. One of the most commonly used expression systems for the production of mammalian membrane proteins is the baculovirus expression system in insect cells. However, a det...

journal_title：Journal of molecular biology

authors： Thomas JA,Tate CG

更新日期：2014-12-12 00:00:00

abstract：:We present evidence for translational activation of the Qbeta coliphage maturation cistron, mediated by the presence of Qbeta replicase. This activation does not require RNA replication, translation of a second gene, or any direct protein-RNA binding at the maturation gene initiation site. Our data support a model in ...

journal_title：Journal of molecular biology

authors： Priano C,Arora R,Jayant L,Mills DR

更新日期：1997-08-22 00:00:00

abstract：:The innermost chorionic layer (ICL) within egg shells of Drosophila melanogaster is composed of thin, abutting three-dimensional crystalline plates which form a closed, membrane-like sheath. Collectively, the crystals within the sheath appear to form a family of related three-dimensional crystals in space group C222; ...

journal_title：Journal of molecular biology

authors： Akey CW,Edelstein SJ

更新日期：1987-02-20 00:00:00

abstract：:The kinetics of the signal recognition particle(SRP)-mediated process of protein translocation across the endoplasmic reticulum membrane was studied by mathematical modeling and complementary experiments. The following results were obtained. (1) A model according to which SRP directs the ribosome, rather than the mRNA...

journal_title：Journal of molecular biology

authors： Rapoport TA,Heinrich R,Walter P,Schulmeister T

更新日期：1987-06-05 00:00:00

abstract：:Essentially all cold agglutinins (CA) with red blood cell I/i specificity isolated from patients with CA disease stemming from lymphoproliferative disorders utilize the VH 4-34 (VH 4-21) gene segment. This near universality of the restricted use of a single gene segment is substantially greater than that demonstrated ...

journal_title：Journal of molecular biology

authors： Li Y,Spellerberg MB,Stevenson FK,Capra JD,Potter KN

更新日期：1996-03-01 00:00:00

abstract：:The yeast retrotransposon Ty1 has structural and functional similarities to retroviruses. We report here that, as in retroviruses, the plus-strand DNA of Ty1 is synthesized as two segments. A central DNA flap is formed during reverse transcription consecutive to elongation (with strand displacement) of the upstream se...

journal_title：Journal of molecular biology

authors： Heyman T,Wilhelm M,Wilhelm FX

更新日期：2003-08-08 00:00:00

abstract：:Helicases are ubiquitous molecular motor proteins that have an important role in the metabolism of nucleic acids. The gene encoding a helicase was cloned from the human malaria parasite Plasmodium falciparum. The polypeptide of 398 amino acid residues has a molecular mass of 45 kDa, contains striking homology to eukar...

journal_title：Journal of molecular biology

authors： Pradhan A,Tuteja R

更新日期：2007-10-19 00:00:00

abstract：:DNA replication fork arrest during the termination phase of chromosome replication in Bacillus subtilis is brought about by the replication terminator protein (RTP) bound to specific DNA terminator sequences (Ter sites) distributed throughout the terminus region. An attractive suggestion by others was that crucial to ...

journal_title：Journal of molecular biology

authors： Duggin IG,Andersen PA,Smith MT,Wilce JA,King GF,Wake RG

更新日期：1999-03-12 00:00:00

abstract：:Transfer RNA su7 G36 is a derivative of tRNA(Trp) with a 3'GUC anticodon complementary to the glutamine codon CAG. This tRNA requires a normally forbidden G-U wobble at the first codon position to suppress a UAG (amber) termination codon. Measurement of amber suppression by mutated su7 G36 tRNAs and correction for tRN...

journal_title：Journal of molecular biology

authors： Schultz DW,Yarus M

更新日期：1994-02-04 00:00:00

abstract：:The majority of mitochondrial proteins can be imported into mitochondria following termination of their translation in the cytosol. Import of fumarase and several other proteins into mitochondria does not appear to occur post-translationally according to standard in vivo and in vitro assays. However, the nature of int...

journal_title：Journal of molecular biology

authors： Karniely S,Regev-Rudzki N,Pines O

更新日期：2006-04-28 00:00:00

abstract：:Aligned sequences from the same family (e.g. the haemoglobins) are seldom representative of the entire family. This is because (1) the sequence databases are heavily skewed toward a small number of organisms and (2) only a minute fraction of all the different family members have been sequenced. For many applications, ...

journal_title：Journal of molecular biology

authors： Sibbald PR,Argos P

更新日期：1990-12-20 00:00:00

abstract：:Intercellular contacts in multicellular organisms are maintained by membrane receptors called cell adhesion molecules (CAMs), which are expressed on cell surfaces. One interesting feature of CAMs is that almost all of their extracellular regions contain repeating copies of structural domains. It is not clear why so ma...

journal_title：Journal of molecular biology

authors： Chen J,Wu Y

更新日期：2017-04-07 00:00:00

abstract：:The origin of metal ion selectivity by members of the SmtB/ArsR family of bacterial metal-sensing transcriptional repressors and the mechanism of negative allosteric regulation of DNA binding is poorly understood. Here, we report that two homologous zinc sensors, Staphylococcus aureus CzrA and cyanobacterial SmtB, are...

journal_title：Journal of molecular biology

authors： Eicken C,Pennella MA,Chen X,Koshlap KM,VanZile ML,Sacchettini JC,Giedroc DP

更新日期：2003-10-31 00:00:00

abstract：:The cis/trans isomerization of prolyl peptide bonds has been suggested to dominate the folding of the alpha subunit of tryptophan synthase from Escherichia coli (alphaTS). To test the role of the unique cis isomer between Asp27 and Pro28, the folding properties of P28A, P28G and G(3)P28G, a three-glycine insertion mut...

journal_title：Journal of molecular biology

authors： Wu Y,Matthews CR

更新日期：2002-09-06 00:00:00

abstract：:In Xenopus somatic cells histone H1 effects the transcriptional repression of oocyte type 5 S RNA genes, without altering the transcription of the somatic type 5 S RNA genes. Using an unambiguous nucleosome mapping method we find substantial differences between the multiple in vitro nucleosome positions on the two typ...

journal_title：Journal of molecular biology

authors： Panetta G,Buttinelli M,Flaus A,Richmond TJ,Rhodes D

更新日期：1998-09-25 00:00:00

abstract：:We use a coarse-grained protein model to characterize the critical nucleus, structural stability, and fibril elongation propensity of Abeta(1-40) oligomers for the C(2x) and C(2z) quaternary forms proposed by solid-state NMR. By estimating equilibrium populations of structurally stable and unstable protofibrils, we de...

journal_title：Journal of molecular biology

authors： Fawzi NL,Okabe Y,Yap EH,Head-Gordon T

更新日期：2007-01-12 00:00:00

abstract：:Iron loss in vitro by the iron scavenger bovine lactoferrin was investigated in acidic media in the presence of three different monoanions (NO(3)(-), Cl(-) and Br(-)) and one dianion (SO(4)(2-)). Holo and monoferric C-site lactoferrins lose iron in acidic media (pH< or =3.5) by a four-step mechanism. The first two ste...

journal_title：Journal of molecular biology

authors： Abdallah FB,El Hage Chahine JM

更新日期：2000-10-20 00:00:00

abstract：:Two-component regulatory systems allow cells to adapt to environmental changes. In Escherichia coli, the TorS/TorR two-component system induces the expression of the tor structural operon encoding the trimethylamine N-oxide reductase respiratory system in response to substrate availability. TorS belongs to a sensor su...

journal_title：Journal of molecular biology

authors： Jourlin C,Ansaldi M,Méjean V

更新日期：1997-04-11 00:00:00

abstract：:Here, we report a new computational method, called sheetminer, for mining beta-sheets in the density maps at intermediate resolutions of 6 to 10A. The method employs a multi-step ad hoc morphological analysis of density maps to identify the unique characteristics of beta-sheets. It was tested on density maps from 12 p...

journal_title：Journal of molecular biology

authors： Kong Y,Ma J

更新日期：2003-09-12 00:00:00

abstract：:The genes for alpha-fetoprotein and albumin arose by duplication of an ancestral gene that contained three genetic domains. These domains were generated by the triplication of a primordial genetic domain composed of five exons or subdomains. That the primordial domain itself arose by amplification of a simpler sequenc...

journal_title：Journal of molecular biology

authors： Alexander F,Young PR,Tilghman SM

更新日期：1984-02-25 00:00:00

abstract：:Calmodulin (CaM) is a calcium binding protein that regulates a wide range of enzymes. Recently the structures of a number of complexes between CaM and synthetic target peptides have been determined. The peptides correspond to the CaM-binding domain of skeletal and smooth muscle myosin light-chain kinase (MLCK) and cal...

journal_title：Journal of molecular biology

authors： Afshar M,Caves LS,Guimard L,Hubbard RE,Calas B,Grassy G,Haiech J

更新日期：1994-12-16 00:00:00

abstract：:The GTPase Center (GAC) RNA domain in bacterial 23S rRNA is directly bound by ribosomal protein L11, and this complex is essential to ribosome function. Previous cocrystal structures of the 58-nucleotide GAC RNA bound to L11 revealed the intricate tertiary fold of the RNA domain, with one monovalent and several divale...

journal_title：Journal of molecular biology

authors： Welty R,Rau M,Pabit S,Dunstan MS,Conn GL,Pollack L,Hall KB

更新日期：2020-02-14 00:00:00

abstract：:The mechanism of initiation of DNA replication in eukaryotic rolling- circle replicons is still poorly understood in molecular terms. Geminiviruses, a family of plant DNA viruses, which use this strategy during part of their replicative cycle, replicate in the nucleus and are amenable to molecular studies. Except for ...

journal_title：Journal of molecular biology

authors： Castellano MM,Sanz-Burgos AP,Gutiérrez C

更新日期：1999-07-16 00:00:00

abstract：:We have previously shown that in Escherichia coli the accumulation of five leucine and three methionine tRNA species is regulated so that those tRNA species that translate major codons increase while those that translate minor codons decrease as the growth rate increases. Here, we have analyzed the growth-rate-depende...

journal_title：Journal of molecular biology

authors： Emilsson V,Näslund AK,Kurland CG

更新日期：1993-03-20 00:00:00

abstract：:Electron crystallography offers an increasingly viable alternative to X-ray crystallography for structure determination, especially for membrane proteins. The methodology has been developed and successfully applied to 2D crystals; however, well-ordered thin, 3D crystals are often produced during crystallization trials...

journal_title：Journal of molecular biology

authors： Shi D,Lewis MR,Young HS,Stokes DL

更新日期：1998-12-18 00:00:00

abstract：:By exploiting the 1/r4 (where r is the separation distance between fluorochromes) dependence of energy transfer between parallel lamellae, we have observed transmembrane energy transfer between membrane receptors and cortical microfilaments. Receptors were labeled with donor- or acceptor-conjugated Fab fragments, wher...

journal_title：Journal of molecular biology

authors： Zhou MJ,Todd RF 3rd,Petty HR

更新日期：1991-03-20 00:00:00