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Ribosomal Protein L11 Selectively Stabilizes a Tertiary Structure of the GTPase Center rRNA Domain.

Abstract：

:The GTPase Center (GAC) RNA domain in bacterial 23S rRNA is directly bound by ribosomal protein L11, and this complex is essential to ribosome function. Previous cocrystal structures of the 58-nucleotide GAC RNA bound to L11 revealed the intricate tertiary fold of the RNA domain, with one monovalent and several divalent ions located in specific sites within the structure. Here, we report a new crystal structure of the free GAC that is essentially identical to the L11-bound structure, which retains many common sites of divalent ion occupation. This new structure demonstrates that RNA alone folds into its tertiary structure with bound divalent ions. In solution, we find that this tertiary structure is not static, but rather is best described as an ensemble of states. While L11 protein cannot bind to the GAC until the RNA has adopted its tertiary structure, new experimental data show that L11 binds to Mg2+-dependent folded states, which we suggest lie along the folding pathway of the RNA. We propose that L11 stabilizes a specific GAC RNA tertiary state, corresponding to the crystal structure, and that this structure reflects the functionally critical conformation of the rRNA domain in the fully assembled ribosome.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Welty R,Rau M,Pabit S,Dunstan MS,Conn GL,Pollack L,Hall KB

doi

10.1016/j.jmb.2019.12.010

subject

Has Abstract

pub_date

2020-02-14 00:00:00

pages

991-1007

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(19)30710-7

journal_volume

432

pub_type

杂志文章

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