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An in-the-groove view of DNA structures in complexes with proteins.

Abstract：

:DNA structures bound to transcription factors are studied by using the crystal coordinates of complexes. Structural characteristics which are found at the sites where a protein secondary structure, an alpha-helix or a beta-sheet binds, can be understood in terms of fitting of the concave surface of the DNA major groove and the convex surface of the protein secondary structure; the former changes, becoming narrower or wider so that it fits the latter. An alpha-helix, independent of which groove it binds to, tends to produce a narrow major groove and a wide minor groove. When the major groove becomes narrower, the DNA helix axis bends around the major groove. Bending of DNA, which is overall structural change, is achieved by changes in local parameters (in particular, the roll parameter) so that an intermediate feature, the groove, fits the protein surface.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Suzuki M,Yagi N

doi

10.1006/jmbi.1996.0055

subject

Has Abstract

pub_date

1996-02-09 00:00:00

pages

677-87

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(96)90055-8

journal_volume

255

pub_type

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