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Effects of 5 cytosine methylation on the B-Z transition in DNA restriction fragments and recombinant plasmids.

Abstract：

:Alternating (dC-dG)n regions in DNA restriction fragments and recombinant plasmids were methylated at the 5 position of the cytosine residues by the HhaI methylase. Methylation lowers the concentration of NaCl or MgCl2 necessary to cause the B-Z conformational transition in these sequences. Ionic strengths higher than physiological conditions are required to form the Z conformation when the methylated (dC-dG)n tract is contiguous with regions that do not form Z structures, in contrast to the results with the DNA polymer poly(m5dC-dG) . poly(m5dC-dG). In supercoiled plasmids containing (dC-dG)n sequences, methylation reduces the number of negative supercoils necessary to stabilize the Z conformation. Calculations of the observed free energy contributions of the B-Z junction and cytosine methylation suggest that two junctions offset the favorable effect of methylation on the Z conformation in (dC-dG)n sequences (about 29 base-pairs in length). Studies with individual methylated topoisomers demonstrate that increasing Na+ concentration up to approximately 0.2 M inhibits the formation of the Z conformation in the (m5dC-dG)n region of supercoiled plasmids. The results suggest that methylation may serve as a triggering mechanism for Z DNA formation in supercoiled DNAs.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Klysik J,Stirdivant SM,Singleton CK,Zacharias W,Wells RD

doi

10.1016/s0022-2836(83)80322-2

subject

Has Abstract

pub_date

1983-07-25 00:00:00

pages

51-71

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)80322-2

journal_volume

168

pub_type

杂志文章

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