Crystallization and preliminary X-ray diffraction results of trichorzianine A 1, a peptide with nineteen residues from Trichoderma harzianum.

abstract：:phi 6 is an enveloped dsRNA bacterial virus. Its segmented genome resides inside the virion associated polymerase complex which is formed by four proteins (P1, P2, P4 and P7) encoded by the viral L segment. Complete and incomplete polymerase complex particles can be produced using cDNA copies of this largest genome se...

journal_title：Journal of molecular biology

authors： Juuti JT,Bamford DH

更新日期：1995-06-09 00:00:00

abstract：:Specific cysteine residues at possible methyl acceptor sites of the Ada protein of Escherichia coli were converted to other amino acids by site-directed mutagenesis of the cloned ada gene of E. coli. Ada protein with the cysteine residue at 321 replaced by alanine was capable of accepting the methyl group from the met...

journal_title：Journal of molecular biology

authors： Takano K,Nakabeppu Y,Sekiguchi M

更新日期：1988-05-20 00:00:00

abstract：:Protein-primed DNA polymerases form a subgroup of the eukaryotic-type DNA polymerases family, also called family B or alpha-like. A multiple amino acid sequence alignment of this subgroup of DNA polymerases led to the identification of two insertions, TPR-1 and TPR-2, in the polymerisation domain. We showed previously...

journal_title：Journal of molecular biology

authors： Dufour E,Rodríguez I,Lázaro JM,de Vega M,Salas M

更新日期：2003-08-22 00:00:00

abstract：:Methyltransferases from the m(1)A(58) tRNA methyltransferase (TrmI) family catalyze the S-adenosyl-l-methionine-dependent N(1)-methylation of tRNA adenosine 58. The crystal structure of Thermus thermophilus TrmI, in complex with S-adenosyl-l-homocysteine, was determined at 1.7 A resolution. This structure is closely r...

journal_title：Journal of molecular biology

authors： Barraud P,Golinelli-Pimpaneau B,Atmanene C,Sanglier S,Van Dorsselaer A,Droogmans L,Dardel F,Tisné C

更新日期：2008-03-21 00:00:00

abstract：:Post-translational modification of cellular proteins by ubiquitin regulates numerous cellular processes, including innate and adaptive immune responses. Ubiquitin-mediated control over these processes can be reversed by cellular deubiquitinating enzymes (DUBs), which remove ubiquitin from cellular targets and depolyme...

journal_title：Journal of molecular biology

authors： Bailey-Elkin BA,Knaap RCM,Kikkert M,Mark BL

更新日期：2017-11-10 00:00:00

abstract：:The expression of xylose utilization in Bacillus subtilis is regulated at the level of transcription by xylose dependent Xyl repressor-xyl operator interaction. We have structurally and functionally characterized the binding sites of Xyl repressor in the xyl regulatory region. Methylation and hydroxyl radical protecti...

journal_title：Journal of molecular biology

authors： Dahl MK,Degenkolb J,Hillen W

更新日期：1994-10-28 00:00:00

abstract：:alpha-Synuclein (alpha-syn) is a 140-residue protein of unknown function, involved in several neurodegenerative disorders, such as Parkinson's disease. Recently, the possible interaction between alpha-syn and polyunsaturated fatty acids has attracted a strong interest. Indeed, lipids are able to trigger the multimeriz...

journal_title：Journal of molecular biology

authors： De Franceschi G,Frare E,Bubacco L,Mammi S,Fontana A,de Laureto PP

更新日期：2009-11-20 00:00:00

abstract：:The solution structure of a 12 base-pair DNA duplex containing the wt-lac promoter Pribnow sequence TATGTT has been studied by two-dimensional nuclear magnetic resonance spectroscopy. Proton assignments for the 24 sugar and base residues were obtained from two-dimensional correlated nuclear magnetic resonance and two-...

journal_title：Journal of molecular biology

authors： Nerdal W,Hare DR,Reid BR

更新日期：1988-06-20 00:00:00

abstract：:The ability of homologous chromosomes (or selected chromosomal loci) to pair specifically in the apparent absence of DNA breakage and recombination represents a prominent feature of eukaryotic biology. The mechanism of homology recognition at the basis of such recombination-independent pairing has remained elusive. A ...

journal_title：Journal of molecular biology

authors： Mazur AK,Nguyen TS,Gladyshev E

更新日期：2020-02-07 00:00:00

abstract：:Gene duplication is a common evolutionary process that leads to the expansion and functional diversification of protein subfamilies. The evolutionary events that cause paralogous proteins to bind different protein ligands (functionally diverged interfaces) are investigated and compared to paralogous proteins that bind...

journal_title：Journal of molecular biology

authors： Aiello D,Caffrey DR

更新日期：2012-10-19 00:00:00

abstract：:Interaction of the leader RNA with the unphosphorylated P-protein has been proposed to play a key role in the transcription-replication transition of Chandipura virus, a model rhabdovirus. Electrophoretic mobility shift assay with the leader RNA and the unphosphorylated P-protein demonstrated existence of two distinct...

journal_title：Journal of molecular biology

authors： Basak S,Polley S,Basu M,Chattopadhyay D,Roy S

更新日期：2004-06-18 00:00:00

abstract：:Pancreatic amyloid is found in more than 95 % of type II diabetes patients. Pancreatic amyloid is formed by the aggregation of islet amyloid polypeptide (hIAPP or amylin), which is a 37-residue peptide. Because pancreatic amyloid is cytotoxic, it is believed that its formation is directly associated with the developme...

journal_title：Journal of molecular biology

authors： Tenidis K,Waldner M,Bernhagen J,Fischle W,Bergmann M,Weber M,Merkle ML,Voelter W,Brunner H,Kapurniotu A

更新日期：2000-01-28 00:00:00

abstract：:Protein tyrosine phosphatase PTPN13, also known as PTP-BL in mice, represents a large multi-domain non-transmembrane scaffolding protein that contains five consecutive PDZ domains. Here, we report the solution structures of the extended murine PTPN13 PDZ3 domain in its apo form and in complex with its physiological li...

journal_title：Journal of molecular biology

authors： Kock G,Dicks M,Yip KT,Kohl B,Pütz S,Heumann R,Erdmann KS,Stoll R

更新日期：2018-10-19 00:00:00

abstract：:Opsins are widespread photoreceptor proteins involved in a diversity of light-driven processes in all major taxa that use the apocarotenoid retinal as a light-absorbing prosthetic group. Proteins from the opsin family are also found in filamentous fungi, but no function has been attributed to them. The fungus Fusarium...

journal_title：Journal of molecular biology

authors： Estrada AF,Avalos J

更新日期：2009-03-20 00:00:00

abstract：:Crystals of NADPH:FMN oxidoreductase from Vibrio harveyi have been obtained and characterized by X-ray diffraction. This enzyme plays a role in the generation of light in luminescent bacteria by providing reduced FMN to luciferase. Large, high quality crystals were grown using polyethylene glycol 6000 at pH 7.0. They ...

journal_title：Journal of molecular biology

authors： Tanner J,Lei B,Liu M,Tu SC,Krause KL

更新日期：1994-08-12 00:00:00

abstract：:Hsc20 is a 20 kDa J-protein that regulates the ATPase activity and peptide-binding specificity of Hsc66, an hsp70-class molecular chaperone. We report herein the crystal structure of Hsc20 from Escherichia coli determined to a resolution of 1.8 A using a combination of single isomorphous replacement (SIR) and multi-wa...

journal_title：Journal of molecular biology

authors： Cupp-Vickery JR,Vickery LE

更新日期：2000-12-15 00:00:00

abstract：:We have developed a computer model for the simulation of simultaneous SELEX against multiple targets. The model assumes equilibrium behavior for the formation of binary ligand:target complexes, and that there is no ligand:ligand or target:target interaction. Target concentrations, ligand concentrations, and affinity d...

journal_title：Journal of molecular biology

authors： Vant-Hull B,Payano-Baez A,Davis RH,Gold L

更新日期：1998-05-08 00:00:00

abstract：:Interactions among Bcl-2 family proteins are important for regulating apoptosis. Prosurvival members of the family interact with proapoptotic BH3 (Bcl-2-homology-3)-only members, inhibiting execution of cell death through the mitochondrial pathway. Structurally, this interaction is mediated by binding of the alpha-hel...

journal_title：Journal of molecular biology

authors： Dutta S,Gullá S,Chen TS,Fire E,Grant RA,Keating AE

更新日期：2010-05-21 00:00:00

abstract：:The Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) functions in splicing group I introns by promoting the formation of the catalytically active structure of the intron's catalytic core. Previous studies suggested a model in which the protein binds first to the intron's P4-P6 domain, and then ...

journal_title：Journal of molecular biology

authors： Caprara MG,Myers CA,Lambowitz AM

更新日期：2001-04-27 00:00:00

abstract：:We have previously isolated sphericase (Sph), an extracellular mesophilic serine protease produced by Bacillus sphaericus. The Sph amino acid sequence is highly homologous to two cold-adapted subtilisins from Antarctic bacilli S39 and S41 (76% and 74% identity, respectively). Sph is calcium-dependent, 310 amino acid r...

journal_title：Journal of molecular biology

authors： Almog O,González A,Klein D,Greenblatt HM,Braun S,Shoham G

更新日期：2003-10-03 00:00:00

abstract：:We describe a hybrid statistical mechanical and dynamical approach for modelling the formation of closed, open and elongating complexes of RNA polymerase, the interactions of these polymerases to produce transcriptional interference, and the regulation of these processes by a DNA-binding and DNA-looping regulatory pro...

journal_title：Journal of molecular biology

authors： Dodd IB,Shearwin KE,Sneppen K

更新日期：2007-06-22 00:00:00

abstract：:Human RNA polymerase II recognizes a strong transcriptional pause signal in the initially transcribed region of HIV-1. We report the use of a limited-step transcription assay to dissect the mechanism underlying recognition of and escape from this HIV-1 pause. Our results suggest that the primary determinant of transcr...

journal_title：Journal of molecular biology

authors： Palangat M,Landick R

更新日期：2001-08-10 00:00:00

abstract：:Two major methods are currently being used to characterize transient intermediates during protein folding at the level of individual residues. Nuclear magnetic resonance (n.m.r.) measurements on the protection of peptide NH hydrogens against exchange with solvent during refolding can provide information about secondar...

journal_title：Journal of molecular biology

authors： Matouschek A,Serrano L,Meiering EM,Bycroft M,Fersht AR

更新日期：1992-04-05 00:00:00

abstract：:Crystals of the mouse major urinary protein (MUP) and rat alpha-2u globulin (AMG) have been grown from solutions of polyethylene glycol 3350 and CdCl2, respectively. The crystals differ both in their morphologies and space groups but have very similar unit cell sizes. AMG crystallized in P2(1) (a = 56.6 A, b = 103.8 A...

journal_title：Journal of molecular biology

authors： Böcskei Z,Findlay JB,North AC,Phillips SE,Somers WS,Wright CE,Lionetti C,Tirindelli R,Cavaggioni A

更新日期：1991-04-20 00:00:00

abstract：:The basic leucine zipper domain of the yeast transcription factor GCN4 consists of a C-terminal leucine zipper and an N-terminal basic DNA-binding region that achieves a stable structure only after association with DNA. Backbone dynamics of a peptide encompassing the basic and leucine zipper bZip domain (residues 226-...

journal_title：Journal of molecular biology

authors： Bracken C,Carr PA,Cavanagh J,Palmer AG 3rd

更新日期：1999-02-05 00:00:00

abstract：:Here we report a deoxyribozyme with a unique structure that contains a two-tiered guanine quadruplex interlinked to a Watson-Crick duplex. Through in vitro selection, sequence mutation, and methylation interference, we show the presence of both the two-tiered guanine-quadruplex and two helical regions contained in the...

journal_title：Journal of molecular biology

authors： McManus SA,Li Y

更新日期：2008-01-25 00:00:00

abstract：:A hydrogen exchange (HX) functional labeling method was used to study allosterically active segments in human hemoglobin (Hb) at the alpha-chain N terminus and the beta-chain C terminus. Allosterically important interactions that contact these segments were removed one or more at a time by mutation (Hbs Cowtown, Bunbu...

journal_title：Journal of molecular biology

authors： Englander JJ,Louie G,McKinnie RE,Englander SW

更新日期：1998-12-18 00:00:00

abstract：:Prion diseases are neurodegenerative disorders caused by the misfolding of the cellular prion protein (PrPC). Gerstmann-Sträussler-Scheinker syndrome is an inherited prion disease with one early-onset allele (HRdup) containing an eight-amino-acid insertion; this LGGLGGYV insert is positioned after valine 129 (human Pr...

journal_title：Journal of molecular biology

authors： Fu ZL,Holmes PC,Westaway D,Sykes BD

更新日期：2019-06-28 00:00:00

abstract：:The minor structural protein (p80), found in about one copy per virion in turnip crinkle virus (TCV), is shown by amino acid analysis and peptide mapping to be a covalent dimer of the major coat protein (p40). The covalent linkage occurs near the N termini of the crosslinked chains. These data suggest that TGV and rel...

journal_title：Journal of molecular biology

authors： Stockley PG,Kirsh AL,Chow EP,Smart JE,Harrison SC

更新日期：1986-10-20 00:00:00

abstract：:We developed a novel genetic method for finding functional regions of a protein by the analysis of chimeras formed between homologous proteins. Sets of chimeric genes were made by intramolecular homologous recombination in a linearized plasmid DNA carrying both recA genes of Escherichia coli and Pseudomonas aeruginosa...

journal_title：Journal of molecular biology

authors： Ogawa T,Shinohara A,Ogawa H,Tomizawa J

更新日期：1992-08-05 00:00:00