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Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination.

Abstract：

:Genetic studies in Saccharomyces cerevisiae predict that the mismatch repair (MMR) factor MSH2-MSH3 binds and stabilizes branched recombination intermediates that form during single strand annealing and gene conversion. To test this model, we constructed a series of DNA substrates that are predicted to form during these recombination events. We show in an electrophoretic mobility shift assay that S. cerevisiae MSH2-MSH3 specifically binds branched DNA substrates containing 3' single-stranded DNA and that ATP stimulates its release from these substrates. Chemical footprinting analyses indicate that MSH2-MSH3 specifically binds at the double-strand/single-strand junction of branched substrates, alters its conformation and opens up the junction. Therefore, MSH2-MSH3 binding to its substrates creates a unique nucleoprotein structure that may signal downstream steps in repair that include interactions with MMR and nucleotide excision repair factors.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Surtees JA,Alani E

doi

10.1016/j.jmb.2006.05.032

subject

Has Abstract

pub_date

2006-07-14 00:00:00

pages

523-36

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(06)00614-0

journal_volume

360

pub_type

杂志文章

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