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Atomic force microscopy of cholera toxin B-oligomers bound to bilayers of biologically relevant lipids.

Abstract：

:Cholera toxin B-oligomer was imaged by atomic force microscopy (AFM) on biologically relevant model membranes, such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine and egg yolk phosphatidylcholine at room temperature in solution at a resolution in the range of 1 to 2 nm. In addition, two-dimensional arrays were grown directly on these model membranes without any special treatment, and were also imaged by AFM. These results demonstrate the ability of AFM for imaging membrane proteins at high resolution without the need of chemical cross-linking, either within the membrane or to the substratum.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Mou J,Yang J,Shao Z

doi

10.1006/jmbi.1995.0238

subject

Has Abstract

pub_date

1995-05-05 00:00:00

pages

507-12

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(85)70238-0

journal_volume

248

pub_type

杂志文章

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