Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing.

Abstract:

:The humoral immune system in higher vertebrates is unique in its ability to generate highly diverse antibody responses against most pathogens as well as against certain malignancies. Several technologies have been developed to exploit this vast source of potentially therapeutic antibodies, including hybridoma technology, phage display and yeast display. Here, we present a novel, high-throughput technology (the Symplex Technology) for rapid direct cloning and identification of human antigen-specific high-affinity antibodies from single antibody-producing cells of immune individuals. The utility of the technology was demonstrated by isolation of diverse sets of unique high-affinity antibodies against tetanus toxoid and influenza virus from immunized volunteers. Hence, the Symplex Technology is a new method for the rapid isolation of high-affinity antibodies directly from humans.

journal_name

J Mol Biol

authors

Meijer PJ,Andersen PS,Haahr Hansen M,Steinaa L,Jensen A,Lantto J,Oleksiewicz MB,Tengbjerg K,Poulsen TR,Coljee VW,Bregenholt S,Haurum JS,Nielsen LS

doi

10.1016/j.jmb.2006.02.040

keywords:

subject

Has Abstract

pub_date

2006-05-05 00:00:00

pages

764-72

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(06)00225-7

journal_volume

358

pub_type

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