Abstract:
:The LH1 light harvesting complex has been purified from a mutant of the photosynthetic bacterium Rhodobacter sphaeroides which synthesizes LH1 as the sole pigment protein. Crystallization trials using polyethylene glycol as the precipitant in the presence of the detergent n-octyl glucoside have resulted in the formation of needle like crystals which diffract beyond 3.5 A and which are relatively resistant to radiation damage. X-ray photographs have established that the crystals belong to the tetragonal system and are probably in space group P4(2)2(1)2. Estimates of the crystal density indicate that the asymmetric unit of the crystals contains two oligomers each with an alpha 6 beta 6 stoichiometry.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Nunn RS,Artymiuk PJ,Baker PJ,Rice DW,Hunter CNdoi
10.1016/0022-2836(92)90331-dkeywords:
subject
Has Abstractpub_date
1992-12-20 00:00:00pages
1259-62issue
4eissn
0022-2836issn
1089-8638pii
0022-2836(92)90331-Djournal_volume
228pub_type
杂志文章,收录出版abstract::The anticancer drug daunomycin has been co-crystallized with the hexanucleotide duplex sequences d(TGTACA) and d(TGATCA) and single crystal X-ray diffraction studies of these two complexes have been carried out. Structure solution of the d(TGTACA) and d(TGATCA) complexes to 1.6 and 1.7 Angstrom resolution, respectivel...
journal_title:Journal of molecular biology
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abstract::The glycolytic enzyme phosphoglycerate kinase (PGK) catalyzes phosphoryl transfer between 1,3-bis-phosphoglycerate and ADP to form 3-phosphoglycerate and ATP. During catalysis, a major hinge bending motion occurs which brings the N and C-terminal enzyme domains and their bound substrates together and in-line for phosp...
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journal_title:Journal of molecular biology
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abstract::Alpha-actinin is the major F-actin crosslinking protein in both muscle and non-muscle cells. We report the crystal structure of the actin binding domain of human muscle alpha-actinin-3, which is formed by two consecutive calponin homology domains arranged in a "closed" conformation. Structural studies and available bi...
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abstract::Network propagation is a powerful tool for genetic analysis which is widely used to identify genes and genetic modules that underlie a process of interest. Here we provide a graphical, web-based platform (http://anat.cs.tau.ac.il/WebPropagate/) in which researchers can easily apply variants of this method to data sets...
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
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更新日期:2007-05-04 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:2000-07-14 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:2008-06-13 00:00:00
abstract::Transcription complexes containing Escherichia coli RNA polymerase terminate RNA synthesis in response to intrinsic (rho-independent) terminators encoded in the DNA template. This type of termination involves the formation of a hairpin structure in the nascent RNA, leading to release of the RNA and polymerase from the...
journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:1994-11-18 00:00:00
abstract::Phosphorylation of short linear peptide motifs is a widespread process for the dynamic regulation of protein-protein interactions. However, the global impact of phosphorylation events on the protein-protein interactome is rarely addressed. The disordered C-terminal tail of ribosomal S6 kinase 1 (RSK1) binds to PDZ dom...
journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/0022-2836(92)90979-t
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/j.jmb.2010.06.034
更新日期:2010-08-20 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:2004-03-26 00:00:00
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journal_title:Journal of molecular biology
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abstract::The bacteriophage T3 and T7 RNA polymerases (RNAP) are closely related, yet exhibit high specificity for their own promoter sequences. In this work the primary determinant of T7 versus T3 promoter specificity has been localized to a single amino acid residue at position 748 in the T7 RNAP. Substitution of this residue...
journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:2004-04-23 00:00:00
abstract::Due to their ability to degrade RNA, selected members of the bovine pancreatic ribonuclease A (RNase A) superfamily are potent cytotoxins. These cytotoxic ribonucleases enter the cytosol of target cells, where they degrade cellular RNA and cause cell death. The cytotoxic activity of most RNases, however, is abolished ...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/j.jmb.2006.03.007
更新日期:2006-05-19 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.1996.0065
更新日期:1996-02-16 00:00:00
abstract::Allosteric regulatory processes are implicated at all levels of biological function. Recent advances in our understanding of the diverse and functionally significant class of intrinsically disordered proteins have identified a multitude of ways in which disordered proteins function within the confines of the allosteri...
journal_title:Journal of molecular biology
pub_type: 杂志文章,评审
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更新日期:2018-08-03 00:00:00
abstract::The aim of the present study was to develop the protein fragment complementation assay (PCA) for the intracellular selection of specific binding molecules from the fully synthetic HuCAL antibody library. Here, we describe the first successful selections of specific antibodies by PCA, and we discuss the opportunities a...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/j.jmb.2005.12.043
更新日期:2006-03-24 00:00:00
abstract::Isolated nucleoli from exponentially growing microplasmodia of Physarum polycephalum were digested with micrococcal nuclease or DNAase I, or were photoreacted with trimethyl psoralen. In the coding region for the precursor of the ribosomal RNA, micrococcal nuclease and DNAase I digestions show predominantly a smear, a...
journal_title:Journal of molecular biology
pub_type: 杂志文章
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abstract::Based on structural analyses and on the behavior of mutants, we suggest that the polymerase domain of HIV-1 reverse transcriptase (RT) plays a critical role in holding and appropriately positioning the template-primer both at the polymerase active site and at the RNase H active site. For RT to successfully copy the vi...
journal_title:Journal of molecular biology
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doi:10.1006/jmbi.1998.1624
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abstract::The human Y RNAs, small RNAs with an unknown function, are complexed with at least three proteins: the 60,000 M(r) Ro protein (Ro60), the 52,000 M(r) Ro protein (Ro52) and the La protein (La). In this study we examined the intermolecular interactions between the components of these so-called Ro ribonucleoprotein (Ro R...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/0022-2836(92)90890-v
更新日期:1992-09-20 00:00:00
abstract::phi 6 is an enveloped dsRNA bacterial virus. Its segmented genome resides inside the virion associated polymerase complex which is formed by four proteins (P1, P2, P4 and P7) encoded by the viral L segment. Complete and incomplete polymerase complex particles can be produced using cDNA copies of this largest genome se...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.1995.0317
更新日期:1995-06-09 00:00:00