The protein synthesis inhibitors, oxazolidinones and chloramphenicol, cause extensive translational inaccuracy in vivo.

Abstract:

:The oxazolidinone family is a new class of synthetic antibiotics that bind to the bacterial 50S ribosomal subunit. Two members of the family, linezolid and XA043, were examined for their effects on translational fidelity using a lacZ reporter gene in vivo. Both promoted highly significant frameshifting and nonsense suppression. Chloramphenicol, a peptidyl transferase inhibitor, affected translational fidelity in a similar fashion. Neither the oxazolidinones nor chloramphenicol stimulated misincorporation of amino acid residues at position 461 in the lacZ gene. In contrast, the aminoglycosides gentamicin and paromomycin, which interact with the decoding region of the 30S subunit, caused significant misincorporation but only modest increases in frameshifting or stop codon readthrough of the lacZ gene. We conclude that effects on translational fidelity may play a significant role in the mechanism of action of the oxazolidinones.

journal_name

J Mol Biol

authors

Thompson J,O'Connor M,Mills JA,Dahlberg AE

doi

10.1016/s0022-2836(02)00784-2

keywords:

subject

Has Abstract

pub_date

2002-09-13 00:00:00

pages

273-9

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022283602007842

journal_volume

322

pub_type

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