Abstract:
:The oxazolidinone family is a new class of synthetic antibiotics that bind to the bacterial 50S ribosomal subunit. Two members of the family, linezolid and XA043, were examined for their effects on translational fidelity using a lacZ reporter gene in vivo. Both promoted highly significant frameshifting and nonsense suppression. Chloramphenicol, a peptidyl transferase inhibitor, affected translational fidelity in a similar fashion. Neither the oxazolidinones nor chloramphenicol stimulated misincorporation of amino acid residues at position 461 in the lacZ gene. In contrast, the aminoglycosides gentamicin and paromomycin, which interact with the decoding region of the 30S subunit, caused significant misincorporation but only modest increases in frameshifting or stop codon readthrough of the lacZ gene. We conclude that effects on translational fidelity may play a significant role in the mechanism of action of the oxazolidinones.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Thompson J,O'Connor M,Mills JA,Dahlberg AEdoi
10.1016/s0022-2836(02)00784-2keywords:
subject
Has Abstractpub_date
2002-09-13 00:00:00pages
273-9issue
2eissn
0022-2836issn
1089-8638pii
S0022283602007842journal_volume
322pub_type
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