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Structure Determination of the Nuclear Pore Complex with Three-Dimensional Cryo electron Microscopy.

Abstract：

:Determining the structure of the nuclear pore complex (NPC) imposes an enormous challenge due to its size, intricate composition and membrane-embedded nature. In vertebrates, about 1000 protein building blocks assemble into a 110-MDa complex that fuses the inner and outer membranes of a cell's nucleus. Here, we review the recent progress in understanding the in situ architecture of the NPC with a specific focus on approaches using three-dimensional cryo electron microscopy. We discuss technological benefits and limitations and give an outlook toward obtaining a high-resolution structure of the NPC.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

von Appen A,Beck M

doi

10.1016/j.jmb.2016.01.004

subject

Has Abstract

pub_date

2016-05-22 00:00:00

pages

2001-10

issue

10 Pt A

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(16)00023-1

journal_volume

428

pub_type

杂志文章,评审

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