A pentameric form of human serum amyloid P component. Crystallization, X-ray diffraction and neutron scattering studies.

abstract：:We report a new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s) (aaRS)/suppressor tRNA pairs. This new system affords higher yields of mutant proteins through the use of both constitutive and induci...

journal_title：Journal of molecular biology

authors： Young TS,Ahmad I,Yin JA,Schultz PG

更新日期：2010-01-15 00:00:00

abstract：:Histone deacetylases (HDACs) modify core histones and participate in large regulatory complexes that both suppress and enhance transcription. Recent studies indicate that some HDACs can act on non-histone proteins as well. Interest in these enzymes is growing because HDAC inhibitors appear to be promising therapeutic ...

journal_title：Journal of molecular biology

authors： Gregoretti IV,Lee YM,Goodson HV

更新日期：2004-04-16 00:00:00

abstract：:The pyrogenic toxin toxic shock syndrome toxin-1 from Staphylococcus aureus is a causative agent of the toxic shock syndrome disease. It belongs to a family of proteins known as superantigens that cross-link major histocompatibility class II molecules and T-cell receptors leading to the activation of a substantial num...

journal_title：Journal of molecular biology

authors： Papageorgiou AC,Brehm RD,Leonidas DD,Tranter HS,Acharya KR

更新日期：1996-07-26 00:00:00

abstract：:Neurofibromatosis type I (NF1) and Legius syndrome are rare inherited disorders that share diagnostic symptoms including dermal abnormalities like axillary and inguinal freckling and café au lait spots. In addition, patients suffering from NF1 have a demanding risk for the development of severe tumors of the periphera...

journal_title：Journal of molecular biology

authors： Führer S,Tollinger M,Dunzendorfer-Matt T

更新日期：2019-09-06 00:00:00

abstract：:Pex19p, a primarily cytosolic protein, is essential for the biogenesis of numerous peroxisomal membrane proteins (PMPs); however, its precise function is unclear. Pex19p might function as a PMP-specific chaperone, a cycling PMP-receptor protein, a PMP membrane insertion factor, or an association/dissociation factor of...

journal_title：Journal of molecular biology

authors： Fransen M,Vastiau I,Brees C,Brys V,Mannaerts GP,Van Veldhoven PP

更新日期：2005-03-11 00:00:00

abstract：:The core shell of hepatitis B virus is a potent immune stimulator, giving a strong neutralizing immune response to foreign epitopes inserted at the immunodominant region, located at the tips of spikes on the exterior of the shell. Here, we analyze structures of core shells with a model epitope inserted at two alternat...

journal_title：Journal of molecular biology

authors： Roseman AM,Borschukova O,Berriman JA,Wynne SA,Pumpens P,Crowther RA

更新日期：2012-10-12 00:00:00

abstract：:The asymmetric unit membrane (AUM) forms numerous plaques covering the apical surface of mammalian urinary bladder epithelium. These plaques contain four major integral membrane proteins called uroplakins Ia, Ib, II and III, which form particles arranged in a well-ordered hexagonal lattice with p6 symmetry and a latti...

journal_title：Journal of molecular biology

authors： Walz T,Häner M,Wu XR,Henn C,Engel A,Sun TT,Aebi U

更新日期：1995-05-19 00:00:00

abstract：:Membrane proteins are unique, in that they can function properly only when they are bound to cellular membranes in a distinct manner. Therefore, positioning of membrane proteins with respect to the membrane is required in addition to the three-dimensional structures in order to understand their detailed molecular mech...

journal_title：Journal of molecular biology

authors： Tatulian SA,Qin S,Pande AH,He X

更新日期：2005-09-02 00:00:00

abstract：:Escherichia coli DsbA belongs to the thioredoxin family and catalyzes the formation of disulfide bonds during the folding of proteins in the bacterial periplasm. It active site (C30-P31-H32-C33) consists of a disulfide bridge that is transferred to newly translocated proteins. The work reported here refers to the DsbA...

journal_title：Journal of molecular biology

authors： Ondo-Mbele E,Vivès C,Koné A,Serre L

更新日期：2005-04-01 00:00:00

abstract：:The segment of the vaccinia DNA topoisomerase from residues 143 to 167 (VGLLTLKNKHIEISPDEIVIKFVGK) is conserved in other members of the eukaryotic type I topoisomerase family. In order to gauge the function of this region, we performed a mutational analysis in which 23 of 25 positions were substituted by alanine. Seve...

journal_title：Journal of molecular biology

authors： Petersen BO,Wittschieben J,Shuman S

更新日期：1996-10-25 00:00:00

abstract：:The control of DNA replication initiation in the plasmid ColE1 is mediated by RNAI, a 108 nucleotide plasmid-encoded RNA that is entirely complementary to the 5'-terminal region of the replication primer RNA. RNAI acts in trans to inhibit primer maturation. Previously, we constructed a plasmid in which the ColE1 RNAI ...

journal_title：Journal of molecular biology

authors： Dooley TP,Tamm J,Polisky B

更新日期：1985-11-05 00:00:00

abstract：:The flagellar basal body of Salmonella typhimurium consists of four rings surrounding a rod. The rod, which is believed to transmit motor rotation to the filament, is not well characterized in terms of its structure and composition. FlgG is known to lie within the distal portion of the rod, in the region where it is s...

journal_title：Journal of molecular biology

authors： Homma M,Kutsukake K,Hasebe M,Iino T,Macnab RM

更新日期：1990-01-20 00:00:00

abstract：:Here, we show that in the chicken genome, the domain of alpha-globin genes is preceded by a CpG island of which the downstream part ( approximately 0.65 kbp) is heavily methylated in lymphoid cells; it is either non-methylated or undermethylated in erythroid cells. Recombinant plasmids were constructed with the corres...

journal_title：Journal of molecular biology

authors： Razin SV,Ioudinkova ES,Scherrer K

更新日期：2000-06-16 00:00:00

abstract：:We produced human leukotriene B(4) (LTB(4)) receptor BLT1 as a recombinant protein in Escherichia coli. This detergent-solubilized receptor displays two states with regard to its affinity for LTB(4): (i) a low-affinity state (K(a)=7.8x10(8)M(-1)) that involves a receptor homodimer (BLT1.LTB(4))(2); we report evidence ...

journal_title：Journal of molecular biology

authors： Banères JL,Parello J

更新日期：2003-06-13 00:00:00

abstract：:The developmental pathways for a variety of eukaryotic and prokaryotic double-stranded DNA viruses include packaging of viral DNA into a preformed procapsid structure, catalyzed by terminase enzymes and fueled by ATP hydrolysis. In most instances, a capsid expansion process accompanies DNA packaging, which significant...

journal_title：Journal of molecular biology

authors： Yang Q,Maluf NK,Catalano CE

更新日期：2008-11-28 00:00:00

abstract：:The number of intrinsically disordered proteins known to be involved in cell-signaling and regulation is growing rapidly. To test for a generalized involvement of intrinsic disorder in signaling and cancer, we applied a neural network predictor of natural disordered regions (PONDR VL-XT) to four protein datasets: huma...

journal_title：Journal of molecular biology

authors： Iakoucheva LM,Brown CJ,Lawson JD,Obradović Z,Dunker AK

更新日期：2002-10-25 00:00:00

abstract：:Although LEDGF/p75 is believed to act as a cellular cofactor of lentiviral integration by tethering integrase (IN) to chromatin, there is no good in vitro model to analyze this functionality. We designed an AlphaScreen assay to study how LEDGF/p75 modulates the interaction of human immunodeficiency virus type 1 IN wit...

journal_title：Journal of molecular biology

authors： McNeely M,Hendrix J,Busschots K,Boons E,Deleersnijder A,Gerard M,Christ F,Debyser Z

更新日期：2011-07-29 00:00:00

abstract：:Mutants of an archaeon Halobacterium halobium, resistant to the universal inhibitor of translation, pactamycin, were isolated. Pactamycin resistance correlated with the presence of mutations in the 16 S rRNA gene of H. halobium single rRNA operon. Three types of mutations were found in pactamycin resistant cells, A694...

journal_title：Journal of molecular biology

authors： Mankin AS

更新日期：1997-11-21 00:00:00

abstract：:The catalytic activity and substrate channeling of the pyridoxal 5'-phosphate-dependent tryptophan synthase alpha(2)beta(2) complex is regulated by allosteric interactions that modulate the switching of the enzyme between open, low activity and closed, high activity states during the catalytic cycle. The highly conser...

journal_title：Journal of molecular biology

authors： Kulik V,Weyand M,Seidel R,Niks D,Arac D,Dunn MF,Schlichting I

更新日期：2002-12-06 00:00:00

abstract：:Endospore formation in Bacillus subtilis is orchestrated by five developmental sigma factors and further modulated by several auxiliary transcription factors. One of these, SpoVT, regulates forespore-specific sigma(G)-dependent genes and plays a key role in the final stages of spore formation. We have determined the c...

journal_title：Journal of molecular biology

authors： Asen I,Djuranovic S,Lupas AN,Zeth K

更新日期：2009-03-06 00:00:00

abstract：:Eukaryotic aminoacyl-tRNA synthetases are usually organized into high-molecular-weight complexes, the structure and function of which are poorly understood. We have previously described a yeast complex containing two aminoacyl-tRNA synthetases, methionyl-tRNA synthetase and glutamyl-tRNA synthetase, and one noncatalyt...

journal_title：Journal of molecular biology

authors： Karanasios E,Simader H,Panayotou G,Suck D,Simos G

更新日期：2007-12-07 00:00:00

abstract：:A key feature of transcriptional attenuation in some amino acid biosynthetic operons is a transcriptional pause that occurs immediately after synthesis of the first leader transcript secondary structure. Both RNA secondary structure and downstream DNA sequence are important for pausing at these sites; however, the pre...

journal_title：Journal of molecular biology

authors： Chan CL,Landick R

更新日期：1993-09-05 00:00:00

abstract：:A 26 Å resolution map of the structure of human low-density lipoprotein (LDL) was obtained from electron cryomicroscopy and single-particle image reconstruction. The structure showed a discoidal-shaped LDL particle with high-density regions mainly distributed at the edge of the particle and low-density regions at the ...

journal_title：Journal of molecular biology

authors： Liu Y,Atkinson D

更新日期：2011-01-07 00:00:00

abstract：:The G.U wobble base-pair in the acceptor helix of Escherichia coli tRNAAlais critical for aminoacylation by the alanine synthetase. Previous work by several groups probed the mechanism of enzyme recognition of G.U by a structure-function analysis of mutant tRNAs using either a cell assay (amber suppressor tRNA) or a t...

journal_title：Journal of molecular biology

authors： McClain WH,Jou YY,Bhattacharya S,Gabriel K,Schneider J

更新日期：1999-07-09 00:00:00

abstract：:Erythropoietin (Epo) is the humoral regulator of red blood-cell production. Low oxygen tension increases the Epo levels by enhancing transcription, through the hypoxia-inducible factor (HIF)-1, a transcriptional modulator in oxygen-regulated gene expression. In the present work, a cooperative interaction between hypox...

journal_title：Journal of molecular biology

authors： Sánchez-Elsner T,Ramírez JR,Sanz-Rodriguez F,Varela E,Bernabéu C,Botella LM

更新日期：2004-02-06 00:00:00

abstract：:The mom gene of bacteriophage Mu, which codes for a DNA modification function, is regulated in a complex manner at both transcriptional and translational levels. The phage-encoded C protein functions as an activator of mom transcription. The mom promoter has features of an activator-dependent weak promoter, and the C ...

journal_title：Journal of molecular biology

authors： Ramesh V,Nagaraja V

更新日期：1996-07-05 00:00:00

abstract：:Single crystals of ribonuclease I from Escherichia coli have been obtained by the vapor diffusion method. The crystals belong to the tetragonal space group P4(1)2(1)2 or its enantiomer P4(3)2(1)2 and have cell parameters a = b = 119.01 A and c = 34.40 A. There is one 27,000 dalton monomer in the asymmetric unit. The c...

journal_title：Journal of molecular biology

authors： Lim LW,Mathur S,Cannistraro VJ,Kennell D

更新日期：1993-11-20 00:00:00

abstract：:We have devised a molecular model building computer program (DEZYMER) which builds new ligand binding sites into a protein of known three-dimensional structure. It alters only the sequence and the side-chain structure of the protein, leaving the protein backbone fold intact by definition. The program searches for a co...

journal_title：Journal of molecular biology

authors： Hellinga HW,Richards FM

更新日期：1991-12-05 00:00:00

abstract：:The gene archb encoding for the cell-bound chitobiase from the Antarctic Gram-positive bacterium Arthrobacter sp. TAD20 was cloned and expressed in Escherichia coli in a soluble form. The mature chitobiase ArChb possesses four functionally independent domains: a catalytic domain stabilized by Ca(2+), a galactose-bindi...

journal_title：Journal of molecular biology

authors： Lonhienne T,Zoidakis J,Vorgias CE,Feller G,Gerday C,Bouriotis V

更新日期：2001-07-06 00:00:00

abstract：:The single mutation L30 K in the Hu-Yap65 WW domain increased the stability of the complex with the peptide GTPPPPYTVG (K(d)=40(+/-5) microM). Here we report the refined solution structure of this complex by NMR spectroscopy and further derived structure-activity relationships by using ligand peptide libraries with tr...

journal_title：Journal of molecular biology

authors： Pires JR,Taha-Nejad F,Toepert F,Ast T,Hoffmüller U,Schneider-Mergener J,Kühne R,Macias MJ,Oschkinat H

更新日期：2001-12-14 00:00:00