Abstract:
:Membranes have a potent ability to promote secondary structure formation in a wide range of membrane-active peptides, believed to be due to a reduction through hydrogen bonding of the energetic cost of partitioning peptide bonds. This process is of fundamental importance for understanding the mechanism of action of toxins and antimicrobial peptides and the stability of membrane proteins. A classic example of membrane-induced folding is the bee-venom peptide melittin that is largely unstructured when free in solution, but strongly adopts an amphipathic alpha-helical conformation when partitioned into membranes. We have determined the energetics of melittin helix formation through measurements of the partitioning free energies and the helicities of native melittin and of a diastereomeric analog with four d-amino acids (d4,l-melittin). Because D4,l-melittin has little secondary structure in either the free or bound forms, it serves as a model for the experimentally inaccessible unfolded bound form of native melittin. The partitioning of native melittin into large unilamellar phosphocholine vesicles is 5.0(+/-0.7) kcal mol-1 more favorable than the partitioning of d4,l-melittin (1 cal=4.186 J). Differences in the circular dichroism spectra of the two forms of melittin indicate that bound native melittin is more helical than bound d4, l-melittin by about 12 residues. These findings disclose that the free energy reduction per residue accompanying the folding of melittin in membrane interfaces is about 0.4 kcal mol-1, consistent with the hypothesis that hydrogen bonding reduces the high cost of partitioning peptide bonds. A value of 0.6 kcal mol-1 per residue has been observed for beta-sheet formation by a hexapeptide model system. These two values provide a useful rule of thumb for estimating the energetic consequences of membrane-induced secondary structure formation.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Ladokhin AS,White SHdoi
10.1006/jmbi.1998.2346subject
Has Abstractpub_date
1999-01-29 00:00:00pages
1363-9issue
4eissn
0022-2836issn
1089-8638pii
S0022-2836(98)92346-4journal_volume
285pub_type
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