Multivalent Interactions by the Set8 Histone Methyltransferase With Its Nucleosome Substrate.

abstract：:In order to study the effect of internal dynamics on the accuracy of NMR structures in detail, we generated NOE distance data from a long molecular dynamics trajectory of BPTI. Cross-relaxation rates were calculated from the trajectory by analysis of the appropriate proton-proton vector autocorrelation functions. A cr...

journal_title：Journal of molecular biology

authors： Schneider TR,Brünger AT,Nilges M

更新日期：1999-01-15 00:00:00

abstract：:Staphylococcus aureus is an opportunistic human pathogen able to transfer virulence genes to other cells through the mobilization of S. aureus pathogenicity islands (SaPIs). SaPIs are derepressed and packaged into phage-like transducing particles by helper phages like 80α or φNM1. Phages 80α and φNM1 encode structural...

journal_title：Journal of molecular biology

authors： Hill RLL,Vlach J,Parker LK,Christie GE,Saad JS,Dokland T

更新日期：2017-05-19 00:00:00

abstract：:Analysis of the conformational differences between the oxy and deoxy forms of hemoglobin is complicated by shifting coordinate systems and correlated motions between different parts of the molecule. Methods independent of any frame of reference were used to study the differences in structure between the oxy and deoxy ...

journal_title：Journal of molecular biology

authors： Nichols WL,Zimm BH,Ten Eyck LF

更新日期：1997-07-25 00:00:00

abstract：:The tetracycline analog 9-(N, N-dimethylglycylamido)-6-demethyl-6-deoxy-tetracycline (9glyTc) belongs to a new group of tetracyclines called glycylcyclines. They are strong antibiotics showing reduced sensitivity against the major tetracycline resistance mechanisms. We have determined the crystal structure of 9glyTc i...

journal_title：Journal of molecular biology

authors： Orth P,Schnappinger D,Sum PE,Ellestad GA,Hillen W,Saenger W,Hinrichs W

更新日期：1999-01-15 00:00:00

abstract：:Transcription of the Bacillus subtilis iol divergon is negatively regulated by a repressor encoded by iolR, which belongs to the DeoR family of bacterial regulators. Gel retardation analysis involving the IolR protein synthesized in Escherichia coli revealed that IolR bound specifically and independently to each of th...

journal_title：Journal of molecular biology

authors： Yoshida KI,Shibayama T,Aoyama D,Fujita Y

更新日期：1999-01-22 00:00:00

abstract：:Incubation of intact reticulocytes with chloramphenicol at concentrations over 10(-3) M caused inhibition of protein synthesis and conversion of polyribosomes to single ribosomes. At 3 x 10(-3) M-chloramphenicol these effects were completely reversible, but reversibility decreased as the chloramphenicol concentration ...

journal_title：Journal of molecular biology

authors： Godchaux W 3rd,Herbert E

更新日期：1966-11-28 00:00:00

abstract：:Synthesis of asparaginyl-tRNA (Asn-tRNA(Asn)) in bacteria can be formed either by directly ligating Asn to tRNA(Asn) using an asparaginyl-tRNA synthetase (AsnRS) or by synthesizing Asn on the tRNA. In the latter two-step indirect pathway, a non-discriminating aspartyl-tRNA synthetase (ND-AspRS) attaches Asp to tRNA(As...

journal_title：Journal of molecular biology

authors： Nair N,Raff H,Islam MT,Feen M,Garofalo DM,Sheppard K

更新日期：2016-02-13 00:00:00

abstract：:Interactions within the decoding center of the 30 S ribosomal subunit have been investigated by constructing all 15 possible mutations at nucleotides C1402 and A1500 in helix 44 of 16 S rRNA. As expected, most of the mutations resulted in highly deleterious phenotypes, consistent with the high degree of conservation o...

journal_title：Journal of molecular biology

authors： Vila-Sanjurjo A,Dahlberg AE

更新日期：2001-05-04 00:00:00

abstract：:Eukaryotic ribosomal RNA genes contain rapidly evolving regions of unknown function termed expansion segments. We present the comparative analysis of the primary and secondary structure of two expansion segments from the large subunit rRNA gene of ten species of Drosophila and the tsetse fly species Glossina morsitans...

journal_title：Journal of molecular biology

authors： Ruiz Linares A,Hancock JM,Dover GA

更新日期：1991-06-05 00:00:00

abstract：:Foot-and-mouth disease virus (FMDV), a non-enveloped picornavirus, is sensitive to acidic conditions. At pH values below 7 the icosahedral virus capsid, formed from 60 copies of a protomer containing four polypeptides (VP1 to 4), dissociates into 12 pentamers, releasing the viral RNA. Evidence suggests that this acid ...

journal_title：Journal of molecular biology

authors： van Vlijmen HW,Curry S,Schaefer M,Karplus M

更新日期：1998-01-16 00:00:00

abstract：:Homotypic death domain (DD)-DD interactions are important in the assembly of oligomeric signaling complexes such as the PIDDosome that acts as a platform for activation of caspase-2-dependent apoptotic signaling. The structure of the PIDDosome core complex exhibits an asymmetric three-layered arrangement containing fi...

journal_title：Journal of molecular biology

authors： Nematollahi LA,Garza-Garcia A,Bechara C,Esposito D,Morgner N,Robinson CV,Driscoll PC

更新日期：2015-02-27 00:00:00

abstract：:The two-chain form of active cathepsin D, a glycosylated, lysosomal aspartic proteinase, has been isolated from human liver. Isoelectric focusing revealed two major species of enzyme that differed by approximately 0.2 pI unit. Crystals suitable for X-ray diffraction analysis were prepared from acidic solutions using p...

journal_title：Journal of molecular biology

authors： Gulnik S,Baldwin ET,Tarasova N,Erickson J

更新日期：1992-09-05 00:00:00

abstract：:Phototaxis in Halobacterium salinarium is the result of an interplay of sensory rhodopsin excitation and adaptation to the stimulus background. Adaptation to orange light, received by sensory rhodopsin I was probed by measuring the behavioral response of cells to a step-like decrease in intensity. Cells were able to a...

journal_title：Journal of molecular biology

authors： Marwan W,Bibikov SI,Montrone M,Oesterhelt D

更新日期：1995-03-03 00:00:00

abstract：:The molecular mechanism by which the microtubule-associated protein (MAP) tau regulates the formation of microtubules (MTs) is poorly understood. The activity of tau is controlled via phosphorylation at specific Ser/Thr sites. Of those phosphorylation sites, 17 precede a proline, making them potential recognition site...

journal_title：Journal of molecular biology

authors： Kutter S,Eichner T,Deaconescu AM,Kern D

更新日期：2016-05-08 00:00:00

abstract：:Catalytic antibodies 6D9 and 9C10, which were induced by immunization with a haptenic transition-state analog (TSA), catalyze the hydrolysis of a nonbioactive chloramphenicol monoester derivative to generate a bioactive chloramphenicol. These antibodies stabilize the transition state to catalyze the hydrolysis reactio...

journal_title：Journal of molecular biology

authors： Oda M,Ito N,Tsumuraya T,Suzuki K,Sakakura M,Fujii I

更新日期：2007-05-25 00:00:00

abstract：:XPB is a superfamily 2 helicase with a 3'-5' polarity. In eukaryotes, XPB is an integral subunit of the transcription factor TFIIH, which plays a dual role in DNA opening at RNA polymerase II promoters and in establishing the repair bubble around a DNA lesion in nucleotide excision repair. Eukaryotic XPB has only very...

journal_title：Journal of molecular biology

authors： Richards JD,Cubeddu L,Roberts J,Liu H,White MF

更新日期：2008-02-22 00:00:00

abstract：:The vitamin D-binding protein, Gc, was purified from human serum and crystallized using the hanging-drop method. The best crystals were grown from 28% polyethylene glycol 400 in 50 mM-sodium acetate at pH 4.8. These crystals diffract to 3.4 A and the observed diffraction is consistent with orthorhombic space groups P4...

journal_title：Journal of molecular biology

authors： Vogelaar NJ,Lindberg U,Schutt CE

更新日期：1991-08-05 00:00:00

abstract：:Cytosolic 5'-nucleotidase III (cN-III) is responsible for selective degradation of pyrimidine 5'-monoribonucleotides during maturation of reticulocytes to erythrocytes. The lack of this enzymatic activity due to genetic aberrations or lead poisoning results in a mild to moderate nonspherocytic hemolytic anemia. In aff...

journal_title：Journal of molecular biology

authors： Grobosky CL,Lopez JB,Rennie S,Skopelitis DJ,Wiest AT,Bingman CA,Bitto E

更新日期：2012-11-02 00:00:00

abstract：:Sequestration of newly replicated origins is one of the mechanisms required to limit initiation of Escherichia coli chromosome replication to once per generation. Origin sequestration lasts for a considerably longer period of time than the sequestration of other newly replicated regions of the chromosome. The reason f...

journal_title：Journal of molecular biology

authors： Bach T,Skarstad K

更新日期：2005-07-01 00:00:00

abstract：:RepA protein, encoded in the Pseudomonas pPS10 replicon, is a stable dimer in solution (dRepA), acting as a self-repressor of repA transcription through binding to an inverted repeat operator. However, RepA monomers (mRepA) are required to initiate plasmid replication upon binding to four directly repeated DNA sequenc...

journal_title：Journal of molecular biology

authors： Díaz-López T,Dávila-Fajardo C,Blaesing F,Lillo MP,Giraldo R

更新日期：2006-12-15 00:00:00

abstract：:Molten globules are partially folded states of proteins which are generally believed to mimic structures formed during the folding process. In order to determine the minimal requirements for the formation of a molten globule state, we have prepared a set of peptide models of the molten globule state of human alpha-lac...

journal_title：Journal of molecular biology

authors： Demarest SJ,Boice JA,Fairman R,Raleigh DP

更新日期：1999-11-19 00:00:00

abstract：:Exocyclic DNA adducts are formed from metabolites of chemical carcinogens and have also been detected as endogenous lesions in human DNA. The exocyclic adduct 3,N(4)-etheno-2'-deoxycytidine (epsilon dC), positioned opposite deoxyguanosine in the B-form duplex of the dodecanucleotide d(CGCGAATTepsilonCGCG), has been cr...

journal_title：Journal of molecular biology

authors： Freisinger E,Fernandes A,Grollman AP,Kisker C

更新日期：2003-06-13 00:00:00

abstract：:The active site of influenza virus neuraminidase (NA) is formed by 11 universally conserved residues. A guanidino group incorporated into two unrelated NA inhibitors was previously reported to occupy different negatively charged sites in the NA active site, A new inhibitor containing two guanidino groups was synthesiz...

journal_title：Journal of molecular biology

authors： Sudbeck EA,Jedrzejas MJ,Singh S,Brouillette WJ,Air GM,Laver WG,Babu YS,Bantia S,Chand P,Chu N,Montgomery JA,Walsh DA,Luo M

更新日期：1997-04-04 00:00:00

abstract：:We describe a selection procedure for construction of very high affinity variants of human growth hormone (hGH) for binding to the extra cellular domain of its receptor (called the hGHbp). Five different libraries of mutated hGH genes (each containing approximately 2 x 10(5) protein variants) were created by randomly ...

journal_title：Journal of molecular biology

authors： Lowman HB,Wells JA

更新日期：1993-12-05 00:00:00

abstract：:C-tailed membrane proteins insert into the bilayer post-translationally because the hydrophobic anchor segment leaves the ribosome at the end of translation. Nevertheless, we find here evidence that the targeting of SciP to the membrane of Escherichia coli occurs co-translationally since signal elements in the N-termi...

journal_title：Journal of molecular biology

authors： Pross E,Soussoula L,Seitl I,Lupo D,Kuhn A

更新日期：2016-10-09 00:00:00

abstract：:Homogeneous detergent-solubilized B873 light-harvesting complexes from a carotenoid-less mutant of the purple non-sulfur bacterium, Rhodospirillum rubrum G9, were reassembled spontaneously into two-dimensional (2D) hexagonal arrays during extensive and controlled dialysis. As the complexes contain only 1 to 2 mol phos...

journal_title：Journal of molecular biology

authors： Ghosh R,Hoenger A,Hardmeyer A,Mihailescu D,Bachofen R,Engel A,Rosenbusch JP

更新日期：1993-05-20 00:00:00

abstract：:We report studies to determine which bacteriophage genes are required for assembly of phage HK97 proheads and what roles they play. We identify the gene encoding the major capsid protein of phage HK97 and report its DNA sequence, together with the DNA sequences of the two genes immediately upstream from it. When the c...

journal_title：Journal of molecular biology

authors： Duda RL,Martincic K,Hendrix RW

更新日期：1995-04-07 00:00:00

abstract：:A large survey of reliably known structures supports the hypothesis that protein loops can behave like elastic strings, and has enabled a parameter describing the entropically generated tensions to be determined. Such tensions may be of major importance in the folding process; here they are discussed mainly in the con...

journal_title：Journal of molecular biology

authors： Thomas DJ

更新日期：1990-11-20 00:00:00

abstract：:The interactions between the complement receptor type 2 (CR2) and the C3 complement fragments C3d, C3dg, and iC3b are essential for the initiation of a normal immune response. A crystal-derived structure of the two N-terminal short consensus repeat (SCR1-2) domains of CR2 in complex with C3d has previously been elucid...

journal_title：Journal of molecular biology

authors： Shaw CD,Storek MJ,Young KA,Kovacs JM,Thurman JM,Holers VM,Hannan JP

更新日期：2010-12-10 00:00:00

abstract：:We have identified the recognition sequence for the Citrobacter freundii restriction endonuclease CfrA, a member of the A-family of type I R-M enzymes. This bipartite target sequence differs in both its components from those of other type I enzymes. We determined the nucleotide sequence of its specificity gene (hsdS) ...

journal_title：Journal of molecular biology

authors： Kannan P,Cowan GM,Daniel AS,Gann AA,Murray NE

更新日期：1989-10-05 00:00:00