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Conservation of organization in the specificity polypeptides of two families of type I restriction enzymes.

Abstract：

:We have identified the recognition sequence for the Citrobacter freundii restriction endonuclease CfrA, a member of the A-family of type I R-M enzymes. This bipartite target sequence differs in both its components from those of other type I enzymes. We determined the nucleotide sequence of its specificity gene (hsdS) and a comparison of this with its relative EcoA identifies two extensive variable regions, an organization analogous to that found in the K-family of type I R-M enzymes. The specificity polypeptides of the A-family, unlike those of K, have an N-terminal conserved region, and this includes a sequence repeated within the central conserved region. A second repeat sequence, identified at the amino acid level, coincides with the only sequence similarity common to all type I S polypeptides. Sequences immediately downstream from the hsdS genes of EcoA, CfrA, EcoK, B and D are almost identical, consistent with an allelic chromosomal location.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kannan P,Cowan GM,Daniel AS,Gann AA,Murray NE

doi

10.1016/0022-2836(89)90001-6

subject

Has Abstract

pub_date

1989-10-05 00:00:00

pages

335-44

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(89)90001-6

journal_volume

209

pub_type

杂志文章

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