Abstract:
:Sequestration of newly replicated origins is one of the mechanisms required to limit initiation of Escherichia coli chromosome replication to once per generation. Origin sequestration lasts for a considerably longer period of time than the sequestration of other newly replicated regions of the chromosome. The reason for this may be the high number of GATC sites present in the origin. Alternatively, other sequence elements in the origin region may be important for its prolonged sequestration. To distinguish between these possibilities we constructed a DNA fragment containing ten GATC sites distributed with the same spacing as the ten GATC sites in the left half of oriC, but with random sequence between the GATC sites, and inserted it at a non-sequestered chromosome location. Sequestration of this GATC-cluster lasted as long as that of oriC, or even longer. The result shows that the presence of ten GATC sites, distributed as in oriC, is sufficient to cause full sequestration, and that other sequence elements most likely do not contribute to sequestration.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Bach T,Skarstad Kdoi
10.1016/j.jmb.2005.04.055keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
7-11issue
1eissn
0022-2836issn
1089-8638pii
S0022-2836(05)00488-2journal_volume
350pub_type
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