The glucose-regulated protein grp94 is related to heat shock protein hsp90.

abstract：:The interaction sites of rabbit skeletal troponin I (TnI) with troponin C (TnC), troponin T (TnT), tropomyosin (Tm) and actin were mapped systematically using nine single cysteine residue TnI mutants with mutation sites at positions 6, 48, 64, 89, 104, 121, 133, 155 or 179 (TnI6, TnI48 etc.). Each mutant was labeled w...

journal_title：Journal of molecular biology

authors： Luo Y,Wu JL,Li B,Langsetmo K,Gergely J,Tao T

更新日期：2000-02-25 00:00:00

abstract：:Retinoic-acid mediated differentiation of F9 cells is accompanied by an increased transcription of the histone H1zero gene. This increase is an early response after addition of retinoic acid, suggesting a direct effect of the hormone on transcription of the gene. We show now that the promoter of histone H1zero contain...

journal_title：Journal of molecular biology

authors： Mader S,Thiele K,Breuer B,Alonso A

更新日期：1994-09-09 00:00:00

abstract：:Anthracycline antibiotics daunomycin and adriamycin are among the most widely used in cancer chemotherapy and DNA is believed to be the primary target of their biological action. The crystal structure of a morpholino derivative of adriamycin bound to the DNA hexamer d(CGTACG) has been determined at 1.5 A resolution. T...

journal_title：Journal of molecular biology

authors： Cirilli M,Bachechi F,Ughetto G,Colonna FP,Capobianco ML

更新日期：1993-04-05 00:00:00

abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

journal_title：Journal of molecular biology

authors： Wei Z,Xue Y,Xu H,Gong W

更新日期：2006-05-26 00:00:00

abstract：:The substrate-binding H-site of human glutathione transferase (GST) M2-2 was subjected to iterative saturation mutagenesis in order to obtain an efficient enzyme with the novel epoxide substrate indene 1,2-oxide. Residues 10, 116, and 210 were targeted, and the activities with the alternative substrates, benzyl isothi...

journal_title：Journal of molecular biology

authors： Norrgård MA,Mannervik B

更新日期：2011-09-09 00:00:00

abstract：:Mutational experiments show how changes in the hydrophobic cores of proteins affect their stabilities. Here, we estimate these effects computationally, using four-body likelihood potentials obtained by simplicial neighborhood analysis of protein packing (SNAPP). In this procedure, the volume of a known protein structu...

journal_title：Journal of molecular biology

authors： Carter CW Jr,LeFebvre BC,Cammer SA,Tropsha A,Edgell MH

更新日期：2001-08-24 00:00:00

abstract：:Crystal structures of the enzyme creatine amidinohydrolase (creatinase, EC 3.5.3.3) with two different inhibitors, the reaction product sarcosine and the substrate creatine, bound have been analyzed by X-ray diffraction methods. With the inhibitor carbamoyl sarcosine, two different crystal forms at different pH values...

journal_title：Journal of molecular biology

authors： Coll M,Knof SH,Ohga Y,Messerschmidt A,Huber R,Moellering H,Rüssmann L,Schumacher G

更新日期：1990-07-20 00:00:00

abstract：:In order to predict the structure of alpha-helical coiled-coil proteins from their sequences, it is necessary to know how the side-chains pack in the interface between the alpha-helical strands. Since in alpha-fibrous proteins leucine is the most common residue at both the a and d positions of the heptad repeat, which...

journal_title：Journal of molecular biology

authors： Offer G,Sessions R

更新日期：1995-06-23 00:00:00

abstract：:The hydrolytic endoribonuclease RNase E, which is widely distributed in bacteria and plants, plays key roles in mRNA degradation and RNA processing in Escherichia coli. The enzymatic activity of RNase E is contained within the conserved amino-terminal half of the 118 kDa protein, and the carboxy-terminal half organize...

journal_title：Journal of molecular biology

authors： Callaghan AJ,Aurikko JP,Ilag LL,Günter Grossmann J,Chandran V,Kühnel K,Poljak L,Carpousis AJ,Robinson CV,Symmons MF,Luisi BF

更新日期：2004-07-23 00:00:00

abstract：:The flagellar basal body of Salmonella typhimurium consists of four rings surrounding a rod. The rod, which is believed to transmit motor rotation to the filament, is not well characterized in terms of its structure and composition. FlgG is known to lie within the distal portion of the rod, in the region where it is s...

journal_title：Journal of molecular biology

authors： Homma M,Kutsukake K,Hasebe M,Iino T,Macnab RM

更新日期：1990-01-20 00:00:00

abstract：:Post-translational modification of cellular proteins by ubiquitin regulates numerous cellular processes, including innate and adaptive immune responses. Ubiquitin-mediated control over these processes can be reversed by cellular deubiquitinating enzymes (DUBs), which remove ubiquitin from cellular targets and depolyme...

journal_title：Journal of molecular biology

authors： Bailey-Elkin BA,Knaap RCM,Kikkert M,Mark BL

更新日期：2017-11-10 00:00:00

abstract：:The backbone dynamics of the EF-hand Ca(2+)-binding protein, calbindin D9k, has been investigated in the apo, (Cd2+)1 and (Ca2+)2 states by measuring the rate constants for amide proton exchange with solvent. 15N-1H correlation spectroscopy was utilized to follow direct 1H-->2H exchange of the slowly exchanging amide ...

journal_title：Journal of molecular biology

authors： Skelton NJ,Kördel J,Akke M,Chazin WJ

更新日期：1992-10-20 00:00:00

abstract：:The histone-like nucleoid structuring (H-NS) protein is a global modulator of gene expression in Gram-negative bacteria. VicH, the H-NS protein of Vibrio cholerae, regulates the expression of certain major virulence determinants implicated in the pathogenesis of cholera. We present here the 2.5A crystal structure of t...

journal_title：Journal of molecular biology

authors： Cerdan R,Bloch V,Yang Y,Bertin P,Dumas C,Rimsky S,Kochoyan M,Arold ST

更新日期：2003-11-21 00:00:00

abstract：:Severe thermal stress induces massive intracellular protein aggregation. The concerted action of Hsp70 (DnaK, DnaJ, GrpE) and Hsp100 (ClpB) chaperones results in solubilization of aggregates followed by reactivation of proteins. It was shown that the Hsp70 chaperone system works at the initial step of the disaggregati...

journal_title：Journal of molecular biology

authors： Lewandowska A,Matuszewska M,Liberek K

更新日期：2007-08-17 00:00:00

abstract：:Integration host factor (IHF) of Escherichia coli is a histone-like protein that is involved both in site-specific recombination and in regulating the expression of a number of phage and bacterial genes. We have shown previously that transcription of the ilvGMEDA operon in E. coli is greatly reduced in IHF mutants. We...

journal_title：Journal of molecular biology

authors： Tsui P,Freundlich M

更新日期：1988-10-05 00:00:00

abstract：:RNA polymerase III transcription of genes with external promoters only (e.g. U6 snRNA) or containing in addition an internal B box (selenocysteine tRNA(Sec)) is stimulated by upstream elements; a distal sequence element (DSE) for U6 or an activator element in the tRNA(Sec) gene. In contrast to the composite structure ...

journal_title：Journal of molecular biology

authors： Myslinski E,Schuster C,Krol A,Carbon P

更新日期：1993-11-20 00:00:00

abstract：:Bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) is an important intracellular signaling molecule that affects diverse physiological processes in bacteria. The intracellular levels of c-di-GMP are controlled by proteins acting as diguanylate cyclase (DGC) and phosphodiesterase (PDE) enzymes that synthesiz...

journal_title：Journal of molecular biology

authors： Pallegar P,Canuti M,Langille E,Peña-Castillo L,Lang AS

更新日期：2020-08-07 00:00:00

abstract：:The crystal structure of abrin-a, a type II ribosome-inactivating protein from the seeds of Abrus precatorius, has been determined from a novel crystalline form by the molecular replacement method using the coordinates of ricin. The structure has been refined at 2.14 A to a R-factor of 18.9%. The root-mean-square devi...

journal_title：Journal of molecular biology

authors： Tahirov TH,Lu TH,Liaw YC,Chen YL,Lin JY

更新日期：1995-07-14 00:00:00

abstract：:Small RNAs were selected from a highly degenerate library on the basis of their ability to bind tightly to Escherichia coli phenylalanyl-tRNA synthetase (FRS). The 63 nucleotide library consisted of the acceptor stem and portions of the D and T stems of E. coli tRNA(Phe) flanking a 32 nucleotide randomized region. Bec...

journal_title：Journal of molecular biology

authors： Peterson ET,Pan T,Coleman J,Uhlenbeck OC

更新日期：1994-09-23 00:00:00

abstract：:The extracellular matrix is a dynamic environment that constantly undergoes remodelling and degradation during vital physiological processes such as angiogenesis, wound healing, and development. Unbalanced extracellular matrix breakdown is associated with many diseases such as arthritis, cancer and fibrosis. Interstit...

journal_title：Journal of molecular biology

authors： Iyer S,Visse R,Nagase H,Acharya KR

更新日期：2006-09-08 00:00:00

abstract：:The role of electrostatic interactions in the stability and the folding of the N-terminal domain of the ribosomal protein L9 (NTL9) was investigated by determining the effects of varying the pH conditions. Urea denaturations and thermal unfolding experiments were used to measure the free energy of folding, DeltaG degr...

journal_title：Journal of molecular biology

authors： Luisi DL,Raleigh DP

更新日期：2000-06-16 00:00:00

abstract：:Our crystal structure of granulysin suggests a mechanism for lysis of bacterial membranes by granulysin, a 74-residue basic protein from human cytolytic T lymphocyte and natural killer cells. We determined the initial crystal structure of selenomethionyl granulysin by MAD phasing at 2A resolution. We present the struc...

journal_title：Journal of molecular biology

authors： Anderson DH,Sawaya MR,Cascio D,Ernst W,Modlin R,Krensky A,Eisenberg D

更新日期：2003-01-10 00:00:00

abstract：:P-type ATPases are involved in the active transport of ions across biological membranes. The KdpFABC complex (P-type ATPase) of Escherichia coli is a high-affinity K+ uptake system that operates only when the cell experiences osmotic stress or K+ limitation. Here, we present the solution structure of the nucleotide bi...

journal_title：Journal of molecular biology

authors： Haupt M,Bramkamp M,Coles M,Altendorf K,Kessler H

更新日期：2004-10-01 00:00:00

abstract：:The rate of ligation of DNA molecules into circular forms depends on the ring closure probability, commonly called the j-factor, which is a sensitive measure of the extent to which thermal fluctuations contribute to bending and twisting of DNA molecules in solution. We present a theoretical treatment of the cyclizatio...

journal_title：Journal of molecular biology

authors： Levene SD,Crothers DM

更新日期：1986-05-05 00:00:00

abstract：:We used site-specific recombination catalyzed by the bacteriophage lambda Int system to probe DNA structure and metabolism in vivo. In vitro, the complexity of catenated products was linearly proportional to substrate supercoil density. A system was developed that gave efficient, controlled Int recombination in Escher...

journal_title：Journal of molecular biology

authors： Bliska JB,Cozzarelli NR

更新日期：1987-03-20 00:00:00

abstract：:Replication of plasmid ColE1 is regulated by a plasmid-specified small RNA (RNA I). RNA I binds to the precursor (RNA II) of the primer for DNA synthesis and inhibits primer formation. The process of binding of RNA I to RNA II that results in formation of a stably bound complex consists of a series of reactions formin...

journal_title：Journal of molecular biology

authors： Tomizawa J

更新日期：1990-04-20 00:00:00

abstract：:Cyanobacteria are shown to be unique in containing membrane-bound manganese superoxide dismutases (MnSOD). They are homodimeric type 2 membrane proteins that protect this phototrophic organism against oxidative stress. We have determined, for the first time, the 2.0A resolution structure of the catalytic portion of th...

journal_title：Journal of molecular biology

authors： Atzenhofer W,Regelsberger G,Jacob U,Peschek G,Furtmüller P,Huber R,Obinger C

更新日期：2002-08-16 00:00:00

abstract：:The sarcin/ricin domain in 23 S/28 S rRNA is crucial for ribosome function, since it constitutes at least part of the binding site for the elongation factors and hence is essential for binding aminoacyl-tRNA and for translocation. The domain is also the site of action of ricin and sarcin and analysis of the effect of ...

journal_title：Journal of molecular biology

authors： Macbeth MR,Wool IG

更新日期：1999-01-15 00:00:00

abstract：:Silk fibroin with the alanyl carboxyl carbon enriched with 13C was obtained by giving a diet containing 13C-enriched alanine to the larvae of Bombyx mori and Antheraea pernyi at the fifth instar. Sericin-free fibroin fibers were prepared from cocoons, and gut was made from the liquid silk in the gland. The final 13C c...

journal_title：Journal of molecular biology

authors： Fujiwara T,Kobayashi Y,Kyogoku Y,Kataoka K

更新日期：1986-01-05 00:00:00

abstract：:Human pseudouridine (Ψ) synthase Pus1 (hPus1) modifies specific uridine residues in several non-coding RNAs: tRNA, U2 spliceosomal RNA, and steroid receptor activator RNA. We report three structures of the catalytic core domain of hPus1 from two crystal forms, at 1.8Å resolution. The structures are the first of a mamm...

journal_title：Journal of molecular biology

authors： Czudnochowski N,Wang AL,Finer-Moore J,Stroud RM

更新日期：2013-10-23 00:00:00