Granulysin crystal structure and a structure-derived lytic mechanism.

abstract：:The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints ...

journal_title：Journal of molecular biology

authors： Schultze P,Wörgötter E,Braun W,Wagner G,Vasák M,Kägi JH,Wüthrich K

更新日期：1988-09-05 00:00:00

abstract：:Ligand-induced cross-linking of the type I interferon (IFN) receptor subunits ifnar1 and ifnar2 induces a pleiotrophic cellular response. Several studies have suggested differential signal activation by flexible recruitment of the accessory receptor subunit ifnar1. We have characterized the roles of the four Ig-like s...

journal_title：Journal of molecular biology

authors： Lamken P,Gavutis M,Peters I,Van der Heyden J,Uzé G,Piehler J

更新日期：2005-07-15 00:00:00

abstract：:Alpha-1 antitrypsin (alpha(1)-AT) is a member of the serpin class of protease inhibitors, and folds to a metastable state rather than its thermodynamically most stable native state. Upon cleavage by a target protease, alpha(1)-AT undergoes a dramatic conformational change to a stable form, translocating the bound prot...

journal_title：Journal of molecular biology

authors： Tsutsui Y,Wintrode PL

更新日期：2007-08-03 00:00:00

abstract：:We have isolated and determined the complete nucleotide sequences of two genes, HrMA4a and HrMA2, which encode the same muscle actin protein of the tunicate Halocynthia roretzi. HrMA4a and HrMA2 contain three exons, and the genes have intron-exon splice junctions at the same positions. The 5' flanking region of HrMA4a...

journal_title：Journal of molecular biology

authors： Kusakabe T,Makabe KW,Satoh N

更新日期：1992-10-05 00:00:00

abstract：:The T7 RNA polymerase promoter has been proposed to contain two domains: the binding region upstream of position -5 is recognized through apparently traditional duplex contacts, while the catalytic domain downstream of position -5 is bound in a melted configuration. This model is tested by following polymerase binding...

journal_title：Journal of molecular biology

authors： Ujvári A,Martin CT

更新日期：1997-11-07 00:00:00

abstract：:The loop between the F and G beta strands (FG loop) of the bacteriophage MS2 coat protein subunit forms inter-subunit contacts around the 5-fold and 3-fold (quasi 6-fold) axes of the T=3 protein shell. In capsids, the loop is found in two very different conformations, one in B subunits, which form the 5-fold contact, ...

journal_title：Journal of molecular biology

authors： Stonehouse NJ,Valegård K,Golmohammadi R,van den Worm S,Walton C,Stockley PG,Liljas L

更新日期：1996-02-23 00:00:00

abstract：:Peptides that bind to major histocompatibility complex products (MHC) are known to exhibit certain sequence motifs which, though common, are neither necessary nor sufficient for binding: MHCs bind certain peptides that do not have the characteristic motifs and only about 30% of the peptides having the required motif, ...

journal_title：Journal of molecular biology

authors： Gulukota K,Sidney J,Sette A,DeLisi C

更新日期：1997-04-18 00:00:00

abstract：:gamma-Glutamyltranspeptidase (EC 2.3.2.2) from Escherichia coli K-12 has been purified and crystallized by means of vapor diffusion in hanging drops. Two kinds of crystals on cell dimensions were found for X-ray diffraction analysis, one from ammonium sulfate and the other from polyethylene glycol 6000 as precipitants...

journal_title：Journal of molecular biology

authors： Kumagai H,Nohara S,Suzuki H,Hashimoto W,Yamamoto K,Sakai H,Sakabe K,Fukuyama K,Sakabe N

更新日期：1993-12-20 00:00:00

abstract：:The tyrosyl-tRNA synthetases from Bacillus stearothermophilus (Bst-TyrTS) and Escherichia coli (Eco-TyrTS) are 56% identical in amino acid sequence. To map and characterize the set of interactions that makes Bst-TyrTS more stable than Eco-TyrTS, a family of nine hybrid proteins was constructed between the two enzymes....

journal_title：Journal of molecular biology

authors： Guez-Ivanier V,Hermann M,Baldwin D,Bedouelle H

更新日期：1993-11-05 00:00:00

abstract：:We report the results of an NMR study of unlabeled and uniformly (13)C,(15)N-labeled d(G(3)AG(2)T(3)G(3)AT) in 100 mM NaCl, conditions under which it forms a dimeric quadruplex containing several new topological features. The DNA oligomer chain in each symmetry-related monomer subunit undergoes three sharp turns to fo...

journal_title：Journal of molecular biology

authors： Zhang N,Gorin A,Majumdar A,Kettani A,Chernichenko N,Skripkin E,Patel DJ

更新日期：2001-08-31 00:00:00

abstract：:Apolipoprotein A-I (ApoA-I) is a protein implicated in the solubilization of lipids and cholesterol from cellular membranes. The study of ApoA-I in phospholipid (PL) monolayers brings relevant information about ApoA-I/PL interactions. We investigated the influence of PL charge and acyl chain organization on the intera...

journal_title：Journal of molecular biology

authors： Chièze L,Bolanos-Garcia VM,Pinot M,Desbat B,Renault A,Beaufils S,Vié V

更新日期：2011-07-01 00:00:00

abstract：:Contact-dependent growth inhibition (CDI) is a widespread mechanism of inter-bacterial competition mediated by the CdiB/CdiA family of two-partner secretion proteins. CdiA effectors carry diverse C-terminal toxin domains (CdiA-CT), which are delivered into neighboring target cells to inhibit growth. CDI(+) bacteria al...

journal_title：Journal of molecular biology

authors： Morse RP,Willett JL,Johnson PM,Zheng J,Credali A,Iniguez A,Nowick JS,Hayes CS,Goulding CW

更新日期：2015-11-20 00:00:00

abstract：:Examination of the first crystal structures of proteins from a halophilic organism suggests that an abundance of acidic residues distributed over the protein surface is a key determinant of adaptation to high-salt conditions. Although one extant theory suggests that acidic residues are favored because of their superio...

journal_title：Journal of molecular biology

authors： Elcock AH,McCammon JA

更新日期：1998-07-24 00:00:00

abstract：:In order to utilize 19F nuclear magnetic resonance (NMR) to probe the solution structure of Escherichia coli tRNAVal labeled by incorporation of 5-fluorouracil, we have assigned its 19F spectrum. We describe here assignments made by examining the spectra of a series of tRNAVal mutants with nucleotide substitutions for...

journal_title：Journal of molecular biology

authors： Chu WC,Feiz V,Derrick WB,Horowitz J

更新日期：1992-10-20 00:00:00

abstract：:Structural analysis of delta131delta, a fragment model of the denatured state of staphylococcal nuclease, has been extended by obtaining long-range distance restraints between chain segments by paramagnetic relaxation enhancement. Fourteen unique PROXYL spin labels were introduced at sites that are solvent-exposed in ...

journal_title：Journal of molecular biology

authors： Gillespie JR,Shortle D

更新日期：1997-04-25 00:00:00

abstract：:Members of the APOBEC (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like) protein family catalyze DNA cytosine deamination and underpin a variety of immune defenses. For instance, several family members, including APOBEC3B (A3B), elicit strong retrotransposon and retrovirus restriction activities. Howev...

journal_title：Journal of molecular biology

authors： Lackey L,Demorest ZL,Land AM,Hultquist JF,Brown WL,Harris RS

更新日期：2012-06-22 00:00:00

abstract：:A family of Structural Maintenance of Chromosome (SMC) complexes is essential for key cellular processes ensuring proper cohesion, condensation and replication. They share a common SMC-kleisin architecture allowing them to embrace DNA. In SMC5/6, the NSE1 and NSE3 KITE and NSE4 kleisin subunits form a stable subcomple...

journal_title：Journal of molecular biology

authors： Adamus M,Lelkes E,Potesil D,Ganji SR,Kolesar P,Zabrady K,Zdrahal Z,Palecek JJ

更新日期：2020-06-12 00:00:00

abstract：:NMR spectroscopy and simulated annealing calculations have been used to determine the three-dimensional structure of NaD1, a novel antifungal and insecticidal protein isolated from the flowers of Nicotiana alata. NaD1 is a basic, cysteine-rich protein of 47 residues and is the first example of a plant defensin from fl...

journal_title：Journal of molecular biology

authors： Lay FT,Schirra HJ,Scanlon MJ,Anderson MA,Craik DJ

更新日期：2003-01-03 00:00:00

abstract：:The portal proteins of tailed bacteriophage and Herpesvirus capsids form dodecameric rings that occupy one capsid vertex and are incorporated during the assembly of capsid precursors called procapsids or proheads. Portals are essential and serve as the pore for DNA transit and the site of tail attachment; however, bac...

journal_title：Journal of molecular biology

authors： Maurer JB,Oh B,Moyer CL,Duda RL

更新日期：2020-03-27 00:00:00

abstract：:The ligation of a decadeoxynucleotide containing the EcoRI recognition site forms a series of multimers which appear to be curved based on observed anomalous gel migration in polyacrylamide gels. The degree of DNA curvature present in the recognition sequence, based upon the observed migration anomaly, can be altered ...

journal_title：Journal of molecular biology

authors： Diekmann S,McLaughlin LW

更新日期：1988-08-20 00:00:00

abstract：:Galanthus nivalis agglutinin (GNA, a 50 kDa tetramer) is a mannose-specific lectin of the Amaryllidaceae family of bulb lectins. Crystal structures of GNA complexed with methyl-alpha-D-mannose (MeMan) and mannose-alpha 1,3-D-mannose-alpha-OMe (MeMan-2) have been determined and analyzed in terms of internal structural ...

journal_title：Journal of molecular biology

authors： Hester G,Wright CS

更新日期：1996-10-04 00:00:00

abstract：:The TonB protein is required for several outer membrane transport processes in bacteria. A short 33-residue peptide segment of TonB has been studied by 1H and 13C nuclear magnetic resonance spectroscopy. The sequence of this peptide segment contains multiple Glu-Pro and Lys-Pro dipeptide repeats that maintain rigid, e...

journal_title：Journal of molecular biology

authors： Brewer S,Tolley M,Trayer IP,Barr GC,Dorman CJ,Hannavy K,Higgins CF,Evans JS,Levine BA,Wormald MR

更新日期：1990-12-20 00:00:00

abstract：:The BTB domain is a widely distributed protein-protein interaction motif that is often found at the N-terminus of zinc finger transcription factors. Previous crystal structures of BTB domains have revealed tightly interwound homodimers, with the N-terminus from one chain forming a two-stranded anti-parallel beta-sheet...

journal_title：Journal of molecular biology

authors： Stogios PJ,Cuesta-Seijo JA,Chen L,Pomroy NC,Privé GG

更新日期：2010-07-30 00:00:00

abstract：:An effective strategy for a plasmid to be maintained in a growing population of bacteria is to prevent the proliferation of newly formed daughter cells that lack the plasmid. In this study, we determined that the promiscuous, incompatibility group P plasmid RK2 encodes such a system. To induce large numbers of RK2-fre...

journal_title：Journal of molecular biology

authors： Jovanovic OS,Ayres EK,Figurski DH

更新日期：1994-03-18 00:00:00

abstract：:We investigated the role of a partially folded intermediate that transiently accumulates during lysozyme folding. Previous studies had shown that the partially folded intermediate is located on a slow-folding pathway and that an additional fast direct pathway from the unfolded state to the native state exists. Kinetic...

journal_title：Journal of molecular biology

authors： Wildegger G,Kiefhaber T

更新日期：1997-07-11 00:00:00

abstract：:Rho-dependent transcription termination is a well-conserved process in bacteria. The Psu and YaeO proteins are the two established inhibitors of the ATP-dependent RNA helicase Rho protein of Escherichia coli. Here, we show a detailed sequence and phylogenetic analysis demonstrating that Vibrio cholerae YaeO (VcYaeO) i...

journal_title：Journal of molecular biology

authors： Pal K,Yadav M,Jain S,Ghosh B,Sen R,Sen U

更新日期：2019-12-06 00:00:00

abstract：:One to four alanines were inserted by site-directed mutagenesis at three different locations within the alpha-helix comprising residues 39 to 50 in bacteriophage T4 lysozyme. All insertion mutants were correctly folded and catalytically active although the insertions led to a thermal destabilization by 1.1 to 4.2 kcal...

journal_title：Journal of molecular biology

authors： Heinz DW,Baase WA,Zhang XJ,Blaber M,Dahlquist FW,Matthews BW

更新日期：1994-02-25 00:00:00

abstract：:Mitochondrial DNA (mtDNA) sequence variation can influence the penetrance of complex diseases and climatic adaptation. While studies in geographically defined human populations suggest that mtDNA mutations become fixed when they have conferred metabolic capabilities optimally suited for a specific environment, it has ...

journal_title：Journal of molecular biology

authors： Dingley SD,Polyak E,Ostrovsky J,Srinivasan S,Lee I,Rosenfeld AB,Tsukikawa M,Xiao R,Selak MA,Coon JJ,Hebert AS,Grimsrud PA,Kwon YJ,Pagliarini DJ,Gai X,Schurr TG,Hüttemann M,Nakamaru-Ogiso E,Falk MJ

更新日期：2014-05-29 00:00:00

abstract：:Human alpha-galactosidase A (alpha-D-galactoside galactohydrolase; EC 3.2.1.22), the glycosylated lysosomal enzyme deficient in Fabry disease, has been crystallized as a complex with the inhibitor N-6-aminohexanoyl-alpha-D-galactopyranosylamine. The "hanging drop" method of vapor diffusion was used to grow crystals fr...

journal_title：Journal of molecular biology

authors： Murali R,Ioannou YA,Desnick RJ,Burnett RM

更新日期：1994-06-17 00:00:00

abstract：:Crystalline complexes of tyrosyl-tRNA synthetase from Bacillus stearothermophilus were prepared with adenosine, AMP, ATP and PPi, all in the presence of tyrosinol, which binds strongly to the tyrosine binding site but cannot be adenylated by ATP. The hydrolysis of ATP in the presence of crystalline tyrosyl-tRNA synthe...

journal_title：Journal of molecular biology

authors： Monteilhet C,Blow DM,Brick P

更新日期：1984-03-15 00:00:00