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Control of ColE1 plasmid replication. Intermediates in the binding of RNA I and RNA II.

Abstract：

:Replication of plasmid ColE1 is regulated by a plasmid-specified small RNA (RNA I). RNA I binds to the precursor (RNA II) of the primer for DNA synthesis and inhibits primer formation. The process of binding of RNA I to RNA II that results in formation of a stably bound complex consists of a series of reactions forming complexes differing in the stability. Formation of a very unstable early intermediate that was previously inferred from the inhibition of stable binding caused by a second RNA I species was firmly established by more extensive studies. This complex is converted to a more stable yet reversible complex that was identified by its RNase sensitivity, which was altered from that of the earlier complex or from that of free RNA I or RNA II. In these complexes, most loops of RNA II interact with their complementary loops of RNA I. The kinetic and structural analyses of the binding process predict formation of a complex interacting at a single pair of complementary loops that precedes formation of these complexes. Thus the process of binding of RNA I to RNA II is seen to consist of a sequence of reactions producing a series of progressively more stable intermediates leading to the final product.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Tomizawa J

doi

10.1016/0022-2836(90)90230-j

subject

Has Abstract

pub_date

1990-04-20 00:00:00

pages

683-94

issue

4

eissn

0022-2836

issn

1089-8638

pii

0022-2836(90)90230-J

journal_volume

212

pub_type

杂志文章

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