Abstract:
:A new method is suggested here for topology prediction of helical transmembrane proteins. The method is based on the hypothesis that the localizations of the transmembrane segments and the topology are determined by the difference in the amino acid distributions in various structural parts of these proteins rather than by specific amino acid compositions of these parts. A hidden Markov model with special architecture was developed to search transmembrane topology corresponding to the maximum likelihood among all the possible topologies of a given protein. The prediction accuracy was tested on 158 proteins and was found to be higher than that found using prediction methods already available. The method successfully predicted all the transmembrane segments in 143 proteins out of the 158, and for 135 of these proteins both the membrane spanning regions and the topologies were predicted correctly. The observed level of accuracy is a strong argument in favor of our hypothesis.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Tusnády GE,Simon Idoi
10.1006/jmbi.1998.2107subject
Has Abstractpub_date
1998-10-23 00:00:00pages
489-506issue
2eissn
0022-2836issn
1089-8638pii
S0022-2836(98)92107-6journal_volume
283pub_type
杂志文章abstract::The human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein (NC) is an essential protein for retroviral replication. Among its numerous functions, NC is a nucleic acid (NA) chaperone protein that catalyzes NA rearrangements leading to the formation of thermodynamically more stable conformations. In vitro, NC ...
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.1995.0403
更新日期:1995-07-28 00:00:00
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journal_title:Journal of molecular biology
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doi:10.1016/j.jmb.2006.09.086
更新日期:2007-01-12 00:00:00
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journal_title:Journal of molecular biology
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doi:10.1016/j.jmb.2007.09.047
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journal_title:Journal of molecular biology
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abstract::By electron microscopy, we have observed immunocomplexes with both negative stain and in amorphous ice using monoclonal antibodies directed against one of the 24 subunits of scorpion haemocyanin. A copy of this subunit occurs at each of the corners of the square-shaped haemocyanin molecule. Three distinct orientations...
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
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