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Integration host factor binds specifically to sites in the ilvGMEDA operon in Escherichia coli.

Abstract：

:Integration host factor (IHF) of Escherichia coli is a histone-like protein that is involved both in site-specific recombination and in regulating the expression of a number of phage and bacterial genes. We have shown previously that transcription of the ilvGMEDA operon in E. coli is greatly reduced in IHF mutants. We report here that IHF specifically protects two sites within the ilvGMEDA promoter-regulatory region against DNase I digestion. These sites are located upstream from the promoter and in the leader region just prior to the sequence that specifies the attenuator. The footprinting experiments and gel retardation assays show that these sites have strong affinity for IHF. These data and results with ilvGMEDA-lac promoter fusions suggest a direct role for IHF in expression of the ilvGMEDA operon.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Tsui P,Freundlich M

doi

10.1016/0022-2836(88)90212-4

subject

Has Abstract

pub_date

1988-10-05 00:00:00

pages

817-20

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(88)90212-4

journal_volume

203

pub_type

杂志文章

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