Role of electrostatic repulsion in the acidic molten globule of cytochrome c.


:The molten globule has been assumed to be a major intermediate state of protein folding. To extend our understanding of protein folding it is important to elucidate the thermodynamic mechanism of conformational stability of the molten globule. To clarify the role of electrostatic charge repulsion in the stability of the acidic molten globule state, we prepared a series of acetylated horse ferricytochrome c species with various degrees of charge repulsion. On the basis of circular dichroism measurement, we show that the stability of the acidic molten globule is determined by a balance of electrostatic repulsions between positive residues, which favor the extended conformation, and the opposing forces, which stabilize the molten globule. These results provide a clear example of charge repulsions producing unfolding of the compact protein structure, and suggest that the reversibly denatured conformation of ferricytochrome c under physiological conditions (i.e. neutral pH, ambient temperature and no denaturant) is the molten globule.


J Mol Biol


Goto Y,Nishikiori S




Has Abstract


1991-12-05 00:00:00














  • Crystallization of porcine liver ribonuclease inhibitor a member of the family of proteins containing leucine-rich repeats.

    abstract::Single crystals of the RNase inhibitor from porcine liver have been obtained from 30 to 34% saturated ammonium sulphate solutions at pH 6.0 to 7.2, containing 20 mM dithiothreitol, at room temperature over a period of two to three weeks. Because the inhibitor contains 30 1/2-cystinyl residues, all of which occur in th...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Neumann U,Hofsteenge J,Arkema AH,Dijkstra BW

    更新日期:1993-05-20 00:00:00

  • S4-16 S ribosomal RNA complex. Binding constant measurements and specific recognition of a 460-nucleotide region.

    abstract::The region of the Escherichia coli 16 S ribosomal RNA recognized by the ribosomal protein S4 has been defined by assaying a set of 13 16 S rRNA fragments for S4 binding. The fragments were prepared by transcription in vitro, and binding constants were measured in three ways: retention of labeled RNA fragments on nitro...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Vartikar JV,Draper DE

    更新日期:1989-09-20 00:00:00

  • Catabolite activator protein-induced DNA bending in transcription initiation.

    abstract::We describe experiments that enable us to track the presence and direction of the DNA bend induced by Escherichia coli catabolite activator protein (CAP) through the intermediate stages of transcription initiation at the lac promoter. Transcriptional complexes examined were formed on superhelical templates to enhance ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Zinkel SS,Crothers DM

    更新日期:1991-05-20 00:00:00

  • A pi-helix switch selective for porphyrin deprotonation and product release in human ferrochelatase.

    abstract::Ferrochelatase (protoheme ferrolyase, EC is the terminal enzyme in heme biosynthesis and catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme IX (heme). Due to the many critical roles of heme, synthesis of heme is required by the vast majority of organisms. Despite significant inv...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Medlock AE,Dailey TA,Ross TA,Dailey HA,Lanzilotta WN

    更新日期:2007-11-02 00:00:00

  • Integral Membrane Enzymes in Eicosanoid Metabolism: Structures, Mechanisms and Inhibitor Design.

    abstract::Eicosanoids are potent lipid mediators involved in central physiological processes such as hemostasis, renal function and parturition. When formed in excess, eicosanoids become critical players in a range of pathological conditions, in particular pain, fever, arthritis, asthma, cardiovascular disease and cancer. Eicos...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章,评审


    authors: Thulasingam M,Haeggström JZ

    更新日期:2020-08-21 00:00:00

  • Crystallographic study of one turn of G/C-rich B-DNA.

    abstract::The DNA decamer d(CCAGGCCTGG) has been studied by X-ray crystallography. At a nominal resolution of 1.6 A, the structure was refined to R = 16.9% using stereochemical restraints. The oligodeoxyribonucleotide forms a straight B-DNA double helix with crystallographic dyad symmetry and ten base-pairs per turn. In the cry...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Heinemann U,Alings C

    更新日期:1989-11-20 00:00:00

  • Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution.

    abstract::To identify structural features in a G-protein-coupled receptor (GPCR) crucial for biosynthesis, stability in the membrane and stability in detergent micelles, we developed an evolutionary approach using expression in the inner membrane of Escherichia coli. From the analysis of 800,000 sequences of the rat neurotensin...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Schlinkmann KM,Hillenbrand M,Rittner A,Künz M,Strohner R,Plückthun A

    更新日期:2012-09-21 00:00:00

  • The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex.

    abstract::Cre recombinase uses two pairs of sequential cleavage and religation reactions to exchange homologous DNA strands between 34 base-pair (bp) LoxP recognition sequences. In the oligomeric recombination complex, a switch between "cleaving" and "non-cleaving" subunit conformations regulates the number, order, and regio-sp...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Martin SS,Pulido E,Chu VC,Lechner TS,Baldwin EP

    更新日期:2002-05-24 00:00:00

  • Flexible stoichiometry and asymmetry of the PIDDosome core complex by heteronuclear NMR spectroscopy and mass spectrometry.

    abstract::Homotypic death domain (DD)-DD interactions are important in the assembly of oligomeric signaling complexes such as the PIDDosome that acts as a platform for activation of caspase-2-dependent apoptotic signaling. The structure of the PIDDosome core complex exhibits an asymmetric three-layered arrangement containing fi...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Nematollahi LA,Garza-Garcia A,Bechara C,Esposito D,Morgner N,Robinson CV,Driscoll PC

    更新日期:2015-02-27 00:00:00

  • The rad50 signature motif: essential to ATP binding and biological function.

    abstract::The repair of double-strand breaks in DNA is an essential process in all organisms, and requires the coordinated activities of evolutionarily conserved protein assemblies. One of the most critical of these is the Mre11/Rad50 (M/R) complex, which is present in all three biological kingdoms, but is not well-understood a...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Moncalian G,Lengsfeld B,Bhaskara V,Hopfner KP,Karcher A,Alden E,Tainer JA,Paull TT

    更新日期:2004-01-23 00:00:00

  • Crystal structure of the N-terminal dimerisation domain of VicH, the H-NS-like protein of Vibrio cholerae.

    abstract::The histone-like nucleoid structuring (H-NS) protein is a global modulator of gene expression in Gram-negative bacteria. VicH, the H-NS protein of Vibrio cholerae, regulates the expression of certain major virulence determinants implicated in the pathogenesis of cholera. We present here the 2.5A crystal structure of t...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Cerdan R,Bloch V,Yang Y,Bertin P,Dumas C,Rimsky S,Kochoyan M,Arold ST

    更新日期:2003-11-21 00:00:00

  • Protein oligomerization through domain swapping: role of inter-molecular interactions and protein concentration.

    abstract::Domain swapping has been shown to be an important mechanism controlling multiprotein assembly and has been suggested recently as a possible mechanism underlying protein aggregation. Understanding oligomerization via domain swapping is therefore of theoretical and practical importance. By using a symmetrized structure-...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Yang S,Levine H,Onuchic JN

    更新日期:2005-09-09 00:00:00

  • Ring closure probabilities for DNA fragments by Monte Carlo simulation.

    abstract::The rate of ligation of DNA molecules into circular forms depends on the ring closure probability, commonly called the j-factor, which is a sensitive measure of the extent to which thermal fluctuations contribute to bending and twisting of DNA molecules in solution. We present a theoretical treatment of the cyclizatio...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Levene SD,Crothers DM

    更新日期:1986-05-05 00:00:00

  • Fluorescence energy transfer distance measurements using site-directed single cysteine mutants. The membrane insertion of colicin A.

    abstract::The ion-channel-forming C-terminal fragment of colicin A binds to negatively charged lipid vesicles and provides an example of insertion of a soluble protein into a lipid bilayer. The soluble structure is known from X-ray crystallography and consists of a ten-helix bundle containing a hydrophobic helical hairpin. In t...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Lakey JH,Baty D,Pattus F

    更新日期:1991-04-05 00:00:00

  • Novel alleles of the Escherichia coli dnaA gene.

    abstract::The Escherichia coli dnaA gene is required for replication of the bacterial chromosome. To identify residues critical for its replication activity, a method to select novel mutations was developed that relied on lytic growth of lambda from an inserted pSC101 replication origin. Replication from the lambda origin was i...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Sutton MD,Kaguni JM

    更新日期:1997-09-05 00:00:00

  • Crystal structure of the agrin-responsive immunoglobulin-like domains 1 and 2 of the receptor tyrosine kinase MuSK.

    abstract::Muscle-specific kinase (MuSK) is a receptor tyrosine kinase expressed exclusively in skeletal muscle, where it is required for formation of the neuromuscular junction. MuSK is activated by agrin, a neuron-derived heparan sulfate proteoglycan. Here, we report the crystal structure of the agrin-responsive first and seco...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Stiegler AL,Burden SJ,Hubbard SR

    更新日期:2006-12-01 00:00:00

  • Analysis of high-resolution electron diffraction patterns from purple membrane labelled with heavy-atoms.

    abstract::Progress in the structure determination of bacteriorhodopsin, the protein component of purple membrane from Halobacterium halobium has been limited by the lack of three-dimensional phase information between 6 and 3 A resolution. By analogy with X-ray methods, it is possible that heavy-atom labelling of the membrane cr...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Ceska TA,Henderson R

    更新日期:1990-06-05 00:00:00

  • Structure, dynamics and thermodynamics of the human centrin 2/hSfi1 complex.

    abstract::Centrin, an EF-hand calcium-binding protein, has been shown to be involved in the duplication of centrosomes, and Sfi1 (Suppressor of fermentation-induced loss of stress resistance protein 1) is one of its centrosomal targets. There are three isoforms of human centrin, but here we only considered centrin 2 (HsCen2). T...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Martinez-Sanz J,Kateb F,Assairi L,Blouquit Y,Bodenhausen G,Abergel D,Mouawad L,Craescu CT

    更新日期:2010-01-08 00:00:00

  • Effects of surrounding sequence on the suppression of nonsense codons.

    abstract::Using a lacI-Z fusion system, we have determined the efficiency of suppression of nonsense codons in the I gene of Escherichia coli by assaying beta-galactosidase activity. We examined the efficiency of four amber suppressors acting on 42 different amber (UAG) codons at known positions in the I gene, and the efficienc...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Miller JH,Albertini AM

    更新日期:1983-02-15 00:00:00

  • Monomer and dimer of Chandipura virus unphosphorylated P-protein binds leader RNA differently: implications for viral RNA synthesis.

    abstract::Interaction of the leader RNA with the unphosphorylated P-protein has been proposed to play a key role in the transcription-replication transition of Chandipura virus, a model rhabdovirus. Electrophoretic mobility shift assay with the leader RNA and the unphosphorylated P-protein demonstrated existence of two distinct...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Basak S,Polley S,Basu M,Chattopadhyay D,Roy S

    更新日期:2004-06-18 00:00:00

  • Photocrosslinking of benzophenone-labeled single cysteine troponin I mutants to other thin filament proteins.

    abstract::The interaction sites of rabbit skeletal troponin I (TnI) with troponin C (TnC), troponin T (TnT), tropomyosin (Tm) and actin were mapped systematically using nine single cysteine residue TnI mutants with mutation sites at positions 6, 48, 64, 89, 104, 121, 133, 155 or 179 (TnI6, TnI48 etc.). Each mutant was labeled w...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Luo Y,Wu JL,Li B,Langsetmo K,Gergely J,Tao T

    更新日期:2000-02-25 00:00:00

  • Crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVAII) hydrolyzing cyclodextrins and pullulan at 2.6 A resolution.

    abstract::The crystal structure of Thermoactinomyces vulgaris R-47 alpha-Amylase II (TVAII) has been determined by multiple isomorphous replacement at 2.6 A resolution. TVAII was crystallized in an orthorhombic system with the space group P212121 and the cell dimensions a=118.5 A, b=119.5 A, c=114.5 A. There are two molecules i...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kamitori S,Kondo S,Okuyama K,Yokota T,Shimura Y,Tonozuka T,Sakano Y

    更新日期:1999-04-16 00:00:00

  • Small-angle X-ray scattering and single-molecule FRET spectroscopy produce highly divergent views of the low-denaturant unfolded state.

    abstract::The results of more than a dozen single-molecule Förster resonance energy transfer (smFRET) experiments suggest that chemically unfolded polypeptides invariably collapse from an expanded random coil to more compact dimensions as the denaturant concentration is reduced. In sharp contrast, small-angle X-ray scattering (...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Yoo TY,Meisburger SP,Hinshaw J,Pollack L,Haran G,Sosnick TR,Plaxco K

    更新日期:2012-05-04 00:00:00

  • Modulation of substrate specificity of the DnaK chaperone by alteration of a hydrophobic arch.

    abstract::Hsp70 chaperones assist protein folding by reversible interaction with extended hydrophobic segments of substrate polypeptides. We investigated the contribution of three structural elements of the substrate- binding cavity of the Escherichia coli homologue, DnaK, to substrate specificity by investigating mutant DnaK p...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Rüdiger S,Mayer MP,Schneider-Mergener J,Bukau B

    更新日期:2000-12-01 00:00:00

  • Human T cell cyclophilin18 binds to thiol-specific antioxidant protein Aop1 and stimulates its activity.

    abstract::Cyclophilins (CyPs) define a family of proteins binding to the immunosuppressive drug cyclosporin A (CsA). They are evolutionary highly conserved proteins being present in both pro- and eukaryotes and in different subcellular locations. CyPs possess enzymatic activity, namely peptidyl-prolyl cis-trans isomerase (PPIas...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Jäschke A,Mi H,Tropschug M

    更新日期:1998-04-10 00:00:00

  • Regulation of aerobic-to-anaerobic transitions by the FNR cycle in Escherichia coli.

    abstract::The FNR (fumarate nitrate reduction) protein plays a central role in the global oxygen response of a variety of bacteria. In Escherichia coli, FNR is the master transcriptional regulator of the transition between aerobic and anaerobic growth. Regulation of FNR is achieved by cycling the molecule between three states i...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Tolla DA,Savageau MA

    更新日期:2010-04-09 00:00:00

  • A model for the mechanism of polymerase translocation.

    abstract::A general mechanism for polymerase translocation is elaborated. The central feature of this mechanism is that a rapid translocational equilibrium is established after each cycle of nucleoside monophosphate incorporation such that the polymerase distributes itself by diffusional sliding between all accessible positions...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Guajardo R,Sousa R

    更新日期:1997-01-10 00:00:00

  • Inhibition of mammary carcinoma cell growth by RXR is mediated by the receptor's oligomeric switch.

    abstract::Ligands that activate the nuclear receptor retinoid X receptor (RXR) display potent anticarcinogenic activities, but the mechanisms by which these compounds inhibit carcinoma cell growth are poorly understood. While RXR can regulate gene expression due to its intrinsic ligand-activated transcription function, this rec...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Yasmin R,Kannan-Thulasiraman P,Kagechika H,Dawson MI,Noy N

    更新日期:2010-04-16 00:00:00

  • Partitioning protein structures into domains: why is it so difficult?

    abstract::This analysis takes an in-depth look into the difficulties encountered by automatic methods for domain decomposition from three-dimensional structure. The analysis involves a multi-faceted set of criteria including the integrity of secondary structure elements, the tendency toward fragmentation of domains, domain boun...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Holland TA,Veretnik S,Shindyalov IN,Bourne PE

    更新日期:2006-08-18 00:00:00

  • The changing face of glucagon fibrillation: structural polymorphism and conformational imprinting.

    abstract::We have established a time-resolved fluorescence assay to study fibrillation of the 29 residue peptide hormone glucagon under a variety of different conditions in a high-throughput format. Fibrils formed at pH 2.5 differ in fibrillation kinetics, morphology, thioflavin T staining and FTIR/CD spectra depending on salts...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Pedersen JS,Dikov D,Flink JL,Hjuler HA,Christiansen G,Otzen DE

    更新日期:2006-01-20 00:00:00