Role of electrostatic repulsion in the acidic molten globule of cytochrome c.

abstract：:The segment of the vaccinia DNA topoisomerase from residues 143 to 167 (VGLLTLKNKHIEISPDEIVIKFVGK) is conserved in other members of the eukaryotic type I topoisomerase family. In order to gauge the function of this region, we performed a mutational analysis in which 23 of 25 positions were substituted by alanine. Seve...

journal_title：Journal of molecular biology

authors： Petersen BO,Wittschieben J,Shuman S

更新日期：1996-10-25 00:00:00

abstract：:We have determined the nucleotide sequence of three positive single-stranded RNA coliphages and have used this information, together with the known sequences of the related phages Q beta and SP, to construct a secondary structure model for the two distal domains of Q beta RNA. The 3' terminal domain, which is about 10...

journal_title：Journal of molecular biology

authors： Beekwilder MJ,Nieuwenhuizen R,van Duin J

更新日期：1995-04-14 00:00:00

abstract：:KorA and KorB proteins of IncP1 plasmid RK2 are encoded in the central control region (ccr) of the plasmid and act as global regulators of plasmid genes for replication, transfer and stable inheritance. KorA represses seven promoters on RK2, by binding to a defined operator site, OA, which always occurs in promoter re...

journal_title：Journal of molecular biology

authors： Kostelidou K,Jones AC,Thomas CM

更新日期：1999-06-04 00:00:00

abstract：:Bulges are common structural motifs in RNA secondary structure and are thought to play important roles in RNA-protein and RNA-drug interactions. Adenosine bases are the most commonly occurring unpaired base in double helical RNA secondary structures. The solution conformation and dynamics of a 25-nucleotide RNA duplex...

journal_title：Journal of molecular biology

authors： Thiviyanathan V,Guliaev AB,Leontis NB,Gorenstein DG

更新日期：2000-07-28 00:00:00

abstract：:A hallmark of the catalytically essential Walker B motif of P-loop NTPases is the presence of an acidic residue (aspartate/glutamate) for efficient Mg2+ coordination. Although the Walker B motif has been identified in well-studied examples of P-loop NTPases, its identity is ambiguous in many families, for example, in ...

journal_title：Journal of molecular biology

authors： Kanade M,Chakraborty S,Shelke SS,Gayathri P

更新日期：2020-09-18 00:00:00

abstract：:A method using binding site "neighbor-effect" parameters (NEPs) is introduced to evaluate the effects of interaction between adjacent ligands on their binding to an infinite linear lattice. Binding site overlap is also taken into account. This enables the conditional probability approach of McGhee & von Hippel to be e...

journal_title：Journal of molecular biology

authors： Wolfe AR,Meehan T

更新日期：1992-02-20 00:00:00

abstract：:We report a new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s) (aaRS)/suppressor tRNA pairs. This new system affords higher yields of mutant proteins through the use of both constitutive and induci...

journal_title：Journal of molecular biology

authors： Young TS,Ahmad I,Yin JA,Schultz PG

更新日期：2010-01-15 00:00:00

abstract：:We describe in this work X-ray scattering and electron microscope studies of rat sciatic and optic nerves as a function of temperature. The scattering experiments were analyzed as described in the previous papers of this series: a variety of parameters were determined, some of which characterize the lattice disorder, ...

journal_title：Journal of molecular biology

authors： Mateu L,Luzzati V,Vonasek E,Mateu E,Villegas GM,Vargas R

更新日期：1995-01-13 00:00:00

abstract：:The pyrogenic toxin toxic shock syndrome toxin-1 from Staphylococcus aureus is a causative agent of the toxic shock syndrome disease. It belongs to a family of proteins known as superantigens that cross-link major histocompatibility class II molecules and T-cell receptors leading to the activation of a substantial num...

journal_title：Journal of molecular biology

authors： Papageorgiou AC,Brehm RD,Leonidas DD,Tranter HS,Acharya KR

更新日期：1996-07-26 00:00:00

abstract：:A putative RNase P RNA gene in camelpox virus, one of the orthopoxviruses, was cloned and transcribed in vitro. No RNase P activity could be detected in vitro from camelpox virus RNase P RNA alone, or by addition of the Escherichia coli RNase P protein subunit to reaction mixtures. Camelpox virus RNase P RNA reconstit...

journal_title：Journal of molecular biology

authors： Yang L,Wesolowski D,Li Y,Altman S

更新日期：2005-12-02 00:00:00

abstract：:RNA polymerase II (Pol II) and its general transcription factors assemble on the promoters of mRNA genes to form large macromolecular complexes that initiate transcription in a regulated manner. During early transcription, these complexes undergo dynamic rearrangement and disassembly as Pol II moves away from the star...

journal_title：Journal of molecular biology

authors： Ly E,Powell AE,Goodrich JA,Kugel JF

更新日期：2020-06-26 00:00:00

abstract：:The Runt domain is the DNA binding domain of the core binding factor (CBF) Runx subunits. The CBFs are transcription factors that play critical roles in hematopoiesis, bone, and neuron development in mammals. A common non-DNA binding CBFbeta subunit heterodimerizes with the Runt domain of the Runx proteins and alloste...

journal_title：Journal of molecular biology

authors： Li Z,Lukasik SM,Liu Y,Grembecka J,Bielnicka I,Bushweller JH,Speck NA

更新日期：2006-12-15 00:00:00

abstract：:Oligonucleotides can bind to double-stranded DNA in a sequence-specific manner to form triple helices. Uniformly modified, pyrimidine-rich oligodeoxyribonuclotides containing internucleosidic N3'-P5' phosphoramidate linkages are known to form very stable triplexes with their DNA target. Psoralen-conjugated triple heli...

journal_title：Journal of molecular biology

authors： Faria M,Wood CD,White MR,Hélène C,Giovannangeli C

更新日期：2001-02-09 00:00:00

abstract：:The hairpin ribozyme belongs to the family of small catalytic RNAs that cleave RNA substrates in a reversible reaction that generates 2',3'-cyclic phosphate and 5'-hydroxyl termini. The hairpin catalytic motif was discovered in the negative strand of the tobacco ringspot virus satellite RNA, where hairpin ribozyme-med...

journal_title：Journal of molecular biology

authors： Fedor MJ

更新日期：2000-03-24 00:00:00

abstract：:Bacterial microcompartments (MCPs) are the simplest organelles known. They function to enhance metabolic pathways by confining several related enzymes inside an all-protein envelope called the shell. In this study, we investigated the factors that govern MCP assembly by performing scanning mutagenesis on the surface r...

journal_title：Journal of molecular biology

authors： Sinha S,Cheng S,Sung YW,McNamara DE,Sawaya MR,Yeates TO,Bobik TA

更新日期：2014-06-12 00:00:00

abstract：:We have examined the equilibrium and kinetic folding properties of two structurally homologous dihydrofolate reductases, Escherichia coli DHFR (EcDHFR) and murine DHFR (MuDHFR), as a function of temperature and ligand concentration. Conformational heterogeneity in native DHFR is well documented, and the results demons...

journal_title：Journal of molecular biology

authors： Clark AC,Frieden C

更新日期：1999-01-29 00:00:00

abstract：:RNA virus behavior can be influenced by interactions among viral genomes and their expression products within the mutant spectra of replicating viral quasispecies. Here, we report the extent of interference of specific capsid and polymerase mutants of foot-and-mouth disease virus (FMDV) on replication of wild-type (wt...

journal_title：Journal of molecular biology

authors： Perales C,Mateo R,Mateu MG,Domingo E

更新日期：2007-06-15 00:00:00

abstract：:We have determined the molecular organization of an ecdysterone-responsive puff site in Drosophila melanogaster. The 71E puff site contains a tightly linked cluster of at least seven genes within a neighborhood of 10 X 10(3) base-pairs. All the genes are expressed in a tissue-specific manner in either the larval or th...

journal_title：Journal of molecular biology

authors： Restifo LL,Guild GM

更新日期：1986-04-20 00:00:00

abstract：:Methyltransferases (MTases) from the Erm family catalyze S-adenosyl-L-methionine-dependent modification of a specific adenine residue in bacterial 23S rRNA, thereby conferring resistance to clinically important macrolide, lincosamide and streptogramin B antibiotics. Despite the available structural data and functional...

journal_title：Journal of molecular biology

authors： Maravić G,Feder M,Pongor S,Flögel M,Bujnicki JM

更新日期：2003-09-05 00:00:00

abstract：:Filamentous phage libraries, displaying 6, 12 or 20 amino acid residue peptides at the N terminus of coat protein pIII were used to define and localize the epitopes of 15 monoclonal antibodies raised against human proenkephalin, a neuropeptide precursor. Eight monoclonal antibodies (PE14 to PE19, PE23 and PE25), which...

journal_title：Journal of molecular biology

authors： Böttger V,Böttger A,Lane EB,Spruce BA

更新日期：1995-04-14 00:00:00

abstract：:As part of an overall effort to map the energetic landscape of the base excision repair pathway, we report the first thermodynamic characterization of repair enzyme binding to lesion-containing duplexes. Isothermal titration calorimetry (ITC) in conjunction with spectroscopic measurements and protease protection assay...

journal_title：Journal of molecular biology

authors： Minetti CA,Remeta DP,Zharkov DO,Plum GE,Johnson F,Grollman AP,Breslauer KJ

更新日期：2003-05-16 00:00:00

abstract：:The eukaryotic 26S proteasome is a large multisubunit complex that degrades the majority of proteins in the cell under normal conditions. The 26S proteasome can be divided into two subcomplexes: the 19S regulatory particle and the 20S core particle. Most substrates are first covalently modified by ubiquitin, which the...

journal_title：Journal of molecular biology

authors： Budenholzer L,Cheng CL,Li Y,Hochstrasser M

更新日期：2017-11-10 00:00:00

abstract：:Fatty acid biosynthesis is essential for the survival of Mycobacterium tuberculosis and acetyl-coenzyme A (acetyl-CoA) is an essential precursor in this pathway. We have determined the 3-D crystal structure of M. tuberculosis citrate lyase beta-subunit (CitE), which as annotated should cleave protein bound citryl-CoA ...

journal_title：Journal of molecular biology

authors： Goulding CW,Bowers PM,Segelke B,Lekin T,Kim CY,Terwilliger TC,Eisenberg D

更新日期：2007-01-12 00:00:00

abstract：:Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) by favoring the activation of a water molecule coordinated to the active-site metal ion. Cys11, His185, Glu222 and Asp233 are the other metal ligands. Wild...

journal_title：Journal of molecular biology

authors： Wang J,Duewel HS,Stuckey JA,Woodard RW,Gatti DL

更新日期：2002-11-22 00:00:00

abstract：:Crystalline complexes of tyrosyl-tRNA synthetase from Bacillus stearothermophilus were prepared with adenosine, AMP, ATP and PPi, all in the presence of tyrosinol, which binds strongly to the tyrosine binding site but cannot be adenylated by ATP. The hydrolysis of ATP in the presence of crystalline tyrosyl-tRNA synthe...

journal_title：Journal of molecular biology

authors： Monteilhet C,Blow DM,Brick P

更新日期：1984-03-15 00:00:00

abstract：:The binding sites for histones and high mobility group proteins (HMG) 14 and 17 have been located on DNA in the nucleosomal cores and H1/H5-containing nucleosomes. The nucleosomes were specifically associated with two molecules of the non-histone proteins HMG 14 and/or HMG 17 when followed by DNA-protein crosslinking ...

journal_title：Journal of molecular biology

authors： Shick VV,Belyavsky AV,Mirzabekov AD

更新日期：1985-09-20 00:00:00

abstract：:The RhaS and RhaR regulatory proteins are encoded in the Escherichia coli L-rhamnose gene cluster. We used complementation analysis and DNA mobility shift assays to show that RhaR is not the direct activator of the L-rhamnose catabolic operon, rhaBAD. An in-frame deletion of rhaS (rhaS-rhaR+) eliminated expression fro...

journal_title：Journal of molecular biology

authors： Egan SM,Schleif RF

更新日期：1993-11-05 00:00:00

abstract：:Adenylate and guanylate cyclases, having different but related substrates, are a paradigm for the study of substrate discrimination. A prokaryotic adenylate cyclase gene, phylogenetically related to eukaryotic counterparts, was screened for mutants remodelling the enzyme's specificity. In a first step, a mutant was se...

journal_title：Journal of molecular biology

authors： Beuve A,Danchin A

更新日期：1992-06-20 00:00:00

abstract：:The two cardiac myosin heavy chain isoforms, alpha and beta, differ functionally, alpha Myosin exhibits higher actin-activated ATPase than does beta myosin, and hearts expressing alpha myosin exhibit increased contractility relative to hearts expressing beta myosin. To understand the molecular basis for this functiona...

journal_title：Journal of molecular biology

authors： McNally EM,Kraft R,Bravo-Zehnder M,Taylor DA,Leinwand LA

更新日期：1989-12-05 00:00:00

abstract：:Chagasin is a protein produced by Trypanosoma cruzi, the parasite that causes Chagas' disease. This small protein belongs to a recently defined family of cysteine protease inhibitors. Although resembling well-known inhibitors like the cystatins in size (110 amino acid residues) and function (they all inhibit papain-li...

journal_title：Journal of molecular biology

authors： Ljunggren A,Redzynia I,Alvarez-Fernandez M,Abrahamson M,Mort JS,Krupa JC,Jaskolski M,Bujacz G

更新日期：2007-08-03 00:00:00