Abstract：
:The molten globule has been assumed to be a major intermediate state of protein folding. To extend our understanding of protein folding it is important to elucidate the thermodynamic mechanism of conformational stability of the molten globule. To clarify the role of electrostatic charge repulsion in the stability of the acidic molten globule state, we prepared a series of acetylated horse ferricytochrome c species with various degrees of charge repulsion. On the basis of circular dichroism measurement, we show that the stability of the acidic molten globule is determined by a balance of electrostatic repulsions between positive residues, which favor the extended conformation, and the opposing forces, which stabilize the molten globule. These results provide a clear example of charge repulsions producing unfolding of the compact protein structure, and suggest that the reversibly denatured conformation of ferricytochrome c under physiological conditions (i.e. neutral pH, ambient temperature and no denaturant) is the molten globule.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Goto Y,Nishikiori Sdoi
10.1016/0022-2836(91)90504-ysubject
Has Abstractpub_date
1991-12-05 00:00:00pages
679-86issue
3eissn
0022-2836issn
1089-8638pii
0022-2836(91)90504-Yjournal_volume
222pub_type
杂志文章abstract：:Analysis of 64 lambda phage proteins revealed the presence of four strong variants of the conserved DNA binding fold of repressors. Three of them have been known from previous studies but the Nu 1 gene product is a new member of the family of proteins that may bind strongly to DNA in the repressor-like fashion. It is ...
journal_title：Journal of molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1006/jmbi.1995.0269
更新日期：1995-05-19 00:00:00
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journal_title：Journal of molecular biology
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更新日期：1994-08-05 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：2004-04-02 00:00:00
abstract：:The gal P2 promoter can be inactivated by point mutations located in the -10 hexamer sequence or immediately upstream from it. Mutations at either site reduce expression in vivo and prevent the formation, in vitro, of tight complexes with RNA polymerase that give a strong footprint and can initiate transcription. Howe...
journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1016/0022-2836(87)90194-x
更新日期：1987-06-05 00:00:00
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更新日期：2006-02-24 00:00:00
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doi：10.1006/jmbi.1999.2590
更新日期：1999-03-19 00:00:00
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更新日期：2005-09-09 00:00:00
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pub_type： 杂志文章
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更新日期：1983-05-25 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章,评审
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更新日期：2019-04-19 00:00:00
abstract：:Nudaurelia capensis omega virus is a T=4, icosahedral virus with a bipartite, positive-sense RNA genome. Expression of the coat protein gene in a baculovirus system was previously shown to result in the formation of procapsids when purified at pH 7.6. Procapsids are round, porous particles (480 A diameter) and have T=...
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1998-11-27 00:00:00
abstract：:Members of the MarR family of winged helix transcriptional regulators have been shown to regulate multidrug and oxidative stress response, pathogenesis, and catabolism of aromatic compounds. Many respond to anionic lipophilic compounds in their capacity to bind DNA, and the co-crystal structure of MarR bound to salicy...
journal_title：Journal of molecular biology
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journal_title：Journal of molecular biology
pub_type： 杂志文章
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更新日期：1999-03-05 00:00:00
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journal_title：Journal of molecular biology
pub_type： 杂志文章
doi：10.1006/jmbi.1998.1500
更新日期：1998-02-13 00:00:00
