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Two-dimensional crystallization of the light-harvesting complex from Rhodospirillum rubrum.

Abstract：

:Homogeneous detergent-solubilized B873 light-harvesting complexes from a carotenoid-less mutant of the purple non-sulfur bacterium, Rhodospirillum rubrum G9, were reassembled spontaneously into two-dimensional (2D) hexagonal arrays during extensive and controlled dialysis. As the complexes contain only 1 to 2 mol phospholipid per mol alpha beta dimer, the arrays formed by a self assembly process are primary due to protein-protein interactions. The hexagonal lattices were analyzed by negative stain electron microscopy and digital image processing. They exhibited a unit cell size of 12.3 nm, in close agreement with the particle diameter of the active photo-unit in native chromatophore membranes. The unit cell contains a central 5 nm stain-filled depression, embraced by a ring with an outer diameter of 10 nm.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Ghosh R,Hoenger A,Hardmeyer A,Mihailescu D,Bachofen R,Engel A,Rosenbusch JP

doi

10.1006/jmbi.1993.1298

subject

Has Abstract

pub_date

1993-05-20 00:00:00

pages

501-4

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(83)71298-2

journal_volume

231

pub_type

杂志文章

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