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A group of repetitive human DNA families that is characterized by extrachromosomal oligomers and restriction-fragment length polymorphism.

Abstract：

:We have recently reported a novel human repetitive DNA (Sau3A family) that exists both in the chromosomes and in the extrachromosomal fraction. Several more clones that hybridized with the Sau3A family were isolated from the extrachromosomal fraction of HeLa cells. Use of these clones as probes has revealed that at least four different types of oligomeric forms of DNA are present in the extrachromosomal fraction. The oligomers consist of one, two, five or 12 subunits of basic 170 base-pair unit DNA, or superimposed forms of two of them. Nucleotide sequencing of these clones indicated that the clones have 70 to 90% sequence homology with human alphoid satellite DNA. These DNA sequences are present also in the chromosomes, as tandemly repeated DNA sequences, and exhibit a considerable degree of restriction-fragment length polymorphism. These results, taken together with the previous findings on Sau3A family DNA, suggest that there is a group of recombination-prone repetitive DNA families in human chromosomes.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kiyama R,Matsui H,Okumura K,Oishi M

doi

10.1016/0022-2836(87)90342-1

subject

Has Abstract

pub_date

1987-02-20 00:00:00

pages

591-7

issue

4

eissn

0022-2836

issn

1089-8638

pii

0022-2836(87)90342-1

journal_volume

193

pub_type

杂志文章

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