Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1.

abstract：:Cytokinesis of animal cells requires the assembly of a contractile ring, which promotes daughter cell splitting. Anillin is a conserved scaffold protein involved in organizing the structural components of the contractile ring including filamentous actin (F-actin), myosin, and septins and in forming the subsequent midb...

journal_title：Journal of molecular biology

authors： Jananji S,Risi C,Lindamulage IKS,Picard LP,Van Sciver R,Laflamme G,Albaghjati A,Hickson GRX,Kwok BH,Galkin VE

更新日期：2017-03-10 00:00:00

abstract：:We have examined the chromatin organization of a cluster of transfer RNA genes at the cytogenic locus 90BC on the right arm of the third Drosophila melanogaster chromosome. We find that the internal promoter sequences are preferentially exposed to micrococcal nuclease and DNase I in chromatin. Moreover, these exposed ...

journal_title：Journal of molecular biology

authors： DeLotto R,Schedl P

更新日期：1984-11-15 00:00:00

abstract：:Interleukin (IL)-23 is a recently identified member of the IL-12 family of heterodimeric cytokines that modulate subpopulations of T helper cells, and both IL-12 and IL-23 are attractive targets for therapy of autoimmune diseases. IL-23 is a binary complex of a four-helix bundle cytokine (p19) and a soluble class I cy...

journal_title：Journal of molecular biology

authors： Lupardus PJ,Garcia KC

更新日期：2008-10-17 00:00:00

abstract：:The X-ray crystal structure of the sulfide derivative of ferric Lucina pectinata hemoglobin component I (HbI) has been determined at 1.9 A resolution (R-factor 0.186). The heme pocket structural organization of HbI is in keeping with its ligand binding properties. The fast sulfide association rate constant can be rela...

journal_title：Journal of molecular biology

authors： Rizzi M,Wittenberg JB,Coda A,Ascenzi P,Bolognesi M

更新日期：1996-04-26 00:00:00

abstract：:We have determined the nucleotide sequence of three positive single-stranded RNA coliphages and have used this information, together with the known sequences of the related phages Q beta and SP, to construct a secondary structure model for the two distal domains of Q beta RNA. The 3' terminal domain, which is about 10...

journal_title：Journal of molecular biology

authors： Beekwilder MJ,Nieuwenhuizen R,van Duin J

更新日期：1995-04-14 00:00:00

abstract：:The endA gene encoding the membrane nuclease of Streptococcus pneumoniae, which is necessary for DNA uptake in genetic transformation, was cloned in a streptococcal vector. This was accomplished by insertional mutagenesis of the gene, cloning of the mutant allele, and substitution of the wild-type allele by chromosoma...

journal_title：Journal of molecular biology

authors： Puyet A,Greenberg B,Lacks SA

更新日期：1990-06-20 00:00:00

abstract：:We have developed a procedure to predict the peptide binding specificity of an SH3 domain from its sequence. The procedure utilizes information extracted from position-specific contacts derived from six SH3/peptide or SH3/protein complexes of known structure. The framework of SH3/peptide contacts defined on the struct...

journal_title：Journal of molecular biology

authors： Brannetti B,Via A,Cestra G,Cesareni G,Helmer-Citterich M

更新日期：2000-04-28 00:00:00

abstract：:DNA gyrase negatively supercoils DNA in a reaction coupled to the binding and hydrolysis of ATP. Limited supercoiling can be achieved in the presence of the non-hydrolysable ATP analogue, 5'-adenylyl beta,gamma-imidodiphosphate (ADPNP). In order to negatively supercoil DNA, gyrase must wrap a length of DNA around itse...

journal_title：Journal of molecular biology

authors： Heddle JG,Mitelheiser S,Maxwell A,Thomson NH

更新日期：2004-03-26 00:00:00

abstract：:BCR-ABL is a chimaeric oncogene generated by translocation of sequences from the c-ABL protein-tyrosine kinase gene on chromosome 9 into the BCR gene on chromosome 22. Alternative chimeric proteins, p210(BCR-ABL) and p190(BCR-ABL), are produced that are characteristic of chronic myelogenous leukemia and acute lymphobl...

journal_title：Journal of molecular biology

authors： Sánchez-García I,Martín-Zanca D

更新日期：1997-03-28 00:00:00

abstract：:We report here the structure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence specificities to BamHI and BglII. BstYI, a thermophilic endonuclease, recognizes and cleaves the degenerate hexanucleotide sequence 5'-RGATCY-3' (where R=A or G and Y=C or T), cleaving DNA after the 5'-R...

journal_title：Journal of molecular biology

authors： Townson SA,Samuelson JC,Vanamee ES,Edwards TA,Escalante CR,Xu SY,Aggarwal AK

更新日期：2004-05-07 00:00:00

abstract：:The essential cell division genes ftsQ, ftsA and ftsZ map in a cluster of cell envelope genes located at two minutes on the Escherichia coli genetic map and appear to constitute an atypical operon. These closely clustered genes are all transcribed in the same direction and yet each of these genes was independently clo...

journal_title：Journal of molecular biology

authors： Yi QM,Rockenbach S,Ward JE Jr,Lutkenhaus J

更新日期：1985-08-05 00:00:00

abstract：:The temperature dependence of processing of precursor tRNA(Gly) (ptRNA(Gly)) variants carrying a single 2'-OCH(3) or locked nucleic acid (LNA) modification at G+1 by Escherichia coli endoribonuclease P RNA was studied at rate-limiting chemistry. We show, for the first time, that these ribose modifications at nucleotid...

journal_title：Journal of molecular biology

authors： Cuzic S,Heidemann KA,Wöhnert J,Hartmann RK

更新日期：2008-05-23 00:00:00

abstract：:The ability of homologous chromosomes (or selected chromosomal loci) to pair specifically in the apparent absence of DNA breakage and recombination represents a prominent feature of eukaryotic biology. The mechanism of homology recognition at the basis of such recombination-independent pairing has remained elusive. A ...

journal_title：Journal of molecular biology

authors： Mazur AK,Nguyen TS,Gladyshev E

更新日期：2020-02-07 00:00:00

abstract：:A novel interrupted gene motif has been reported in which a 50-nucleotide insertion into bacteriophage T4 gene 60 appears to be present in the message at the time of translation, yet it is not translated. We present here a dynamic model for how translation may be occurring in the neighborhood of the interruption. The ...

journal_title：Journal of molecular biology

authors： Burke-Agüero DH,Hearst JE

更新日期：1990-05-20 00:00:00

abstract：:A new affinity chromatographic procedure for the separation of transcriptionally active nucleosomes has been used to study the changes that take place in chromatin structure along the c-fos and c-myc genes when RNA synthesis is inhibited. Mercury-affinity chromatography separates the sulfhydryl-reactive nucleosomes of...

journal_title：Journal of molecular biology

authors： Chen TA,Sterner R,Cozzolino A,Allfrey VG

更新日期：1990-04-05 00:00:00

abstract：:During protein biosynthesis in bacteria, one of the earliest events that a nascent polypeptide chain goes through is the co-translational enzymatic processing. The event includes two enzymatic pathways: deformylation of the N-terminal methionine by the enzyme peptide deformylase (PDF), followed by methionine excision ...

journal_title：Journal of molecular biology

authors： Bhakta S,Akbar S,Sengupta J

更新日期：2019-03-29 00:00:00

abstract：:In bacteriophages of the cystovirus family, the polymerase complex (PX) encodes a 75-kDa RNA-directed RNA polymerase (P2) that transcribes the double-stranded RNA genome. Also a constituent of the PX is the essential protein P7 that, in addition to accelerating PX assembly and facilitating genome packaging, plays a re...

journal_title：Journal of molecular biology

authors： Alphonse S,Arnold JJ,Bhattacharya S,Wang H,Kloss B,Cameron CE,Ghose R

更新日期：2014-07-15 00:00:00

abstract：:Cross-links in tendon collagen are essential for the biomechanical strength of healthy tissue. The nature and position of these cross-links has long been a subject for conjecture. We have approached this problem in a non-destructive manner, by studying neutron diffraction from collagen fibrils that have been specifica...

journal_title：Journal of molecular biology

authors： Wess TJ,Miller A,Bradshaw JP

更新日期：1990-05-05 00:00:00

abstract：:The U6 RNA internal stem-loop (U6 ISL) is a highly conserved domain of the spliceosome that is important for pre-mRNA splicing. The U6 ISL contains an internal loop that is in equilibrium between two conformations controlled by the protonation state of an adenine (pK(a)=6.5). Lower pH favors formation of a protonated ...

journal_title：Journal of molecular biology

authors： Venditti V,Clos L 2nd,Niccolai N,Butcher SE

更新日期：2009-09-04 00:00:00

abstract：:Dipicolinic acid (DPA) is a small polar molecule that accumulates to high concentrations in bacterial endospores, and is thought to play a role in spore heat resistance, or the maintenance of heat resistance. Previous work has shown that mutations in the spoVF locus of Bacillus subtilis prevent the formation of DPA, a...

journal_title：Journal of molecular biology

authors： Daniel RA,Errington J

更新日期：1993-07-20 00:00:00

abstract：:The multi-domain serine protease C2 provides the catalytic activity for the C3 and C5- convertases of the classical and lectin pathways of complement activation. Formation of these convertases requires the Mg(2+)-dependent binding of C2 to C4b, and the subsequent cleavage of C2 by C1s or MASP2, respectively. The C-ter...

journal_title：Journal of molecular biology

authors： Krishnan V,Xu Y,Macon K,Volanakis JE,Narayana SV

更新日期：2007-03-16 00:00:00

abstract：:In eukaryotes, cellular levels of adenosine monophosphate (AMP) signal the metabolic state of the cell. AMP concentrations increase significantly upon metabolic stress, such as glucose deprivation in yeast. Here, we show that several DEAD-box RNA helicases are sensitive to AMP, which is not produced during ATP hydroly...

journal_title：Journal of molecular biology

authors： Putnam AA,Jankowsky E

更新日期：2013-10-23 00:00:00

abstract：:Understanding the interaction of Arf and Hdm2 has recently become a central issue in cancer biology. In response to hyperproliferative signals, p14(Arf) stabilizes p53 by binding to Hdm2 and inhibits the ubiquitination and subsequent proteosome-dependent degradation of p53. The medical importance of the Arf-Hdm2-p53 r...

journal_title：Journal of molecular biology

authors： Bothner B,Lewis WS,DiGiammarino EL,Weber JD,Bothner SJ,Kriwacki RW

更新日期：2001-11-23 00:00:00

abstract：:The nuclear localized, multi-subunit COP9 complex (or COP9 signalosome) is a key developmental modulator involved in repression of photomorphogenesis. In an effort to further define the molecular actions of the COP9 complex, a yeast two hybrid interactive screen was undertaken to identify proteins that could directly ...

journal_title：Journal of molecular biology

authors： Kwok SF,Staub JM,Deng XW

更新日期：1999-01-08 00:00:00

abstract：:Specific cysteine residues at possible methyl acceptor sites of the Ada protein of Escherichia coli were converted to other amino acids by site-directed mutagenesis of the cloned ada gene of E. coli. Ada protein with the cysteine residue at 321 replaced by alanine was capable of accepting the methyl group from the met...

journal_title：Journal of molecular biology

authors： Takano K,Nakabeppu Y,Sekiguchi M

更新日期：1988-05-20 00:00:00

abstract：:Antimycin A (antimycin), one of the first known and most potent inhibitors of the mitochondrial respiratory chain, binds to the quinone reduction site of the cytochrome bc1 complex. Structure-activity relationship studies have shown that the N-formylamino-salicyl-amide group is responsible for most of the binding spec...

journal_title：Journal of molecular biology

authors： Huang LS,Cobessi D,Tung EY,Berry EA

更新日期：2005-08-19 00:00:00

abstract：:Amber suppressors previously isolated from the yeast Saccharomyces cerevisiae and belonging to the same phenotypic class (Liebman et al., 1976) were assigned to nine different linkage groups named SUP52 through SUP60. One of these suppressors, SUP52, had been shown to cause the insertion of leucine and had been geneti...

journal_title：Journal of molecular biology

authors： Liebman SW,Srodulski Z,Reed CR,Stewart JW,Sherman F,Brennan G

更新日期：1984-09-15 00:00:00

abstract：:Two crystal habits, one rod shaped and the other square prismatic, of the Fab fragment of a monoclonal anti-phenylalanine hydroxylase antibody have been grown using the method of vapour phase diffusion against polyethylene glycol 6000. The square prisms diffract to better than 2.8 A, belong to the space group P1 and h...

journal_title：Journal of molecular biology

authors： Guddat LW,Cowan SW,Machin KJ,Isaacs NW,Cotton RG

更新日期：1985-11-20 00:00:00

abstract：:The inherent heterogeneity of many protein assemblies complicates characterization of their structure and dynamics, as most biophysical techniques require homogeneous preparations of isolated components. For this reason, quantitative studies of the molecular chaperone αB-crystallin, which populates a range of intercon...

journal_title：Journal of molecular biology

authors： Baldwin AJ,Lioe H,Robinson CV,Kay LE,Benesch JL

更新日期：2011-10-21 00:00:00

abstract：:Archaebacterial respiratory chains are poorly understood at the molecular level. We have cloned and sequenced a cluster of five new genes from the archaebacterium Sulfolobus acidocaldarius, four of them coding for redox proteins: a Rieske iron-sulphur protein, a cytochrome b, a subunit II of cytochrome oxidase and a b...

journal_title：Journal of molecular biology

authors： Castresana J,Lübben M,Saraste M

更新日期：1995-07-07 00:00:00