Regulation of Bcl-2 gene expression by BCR-ABL is mediated by Ras.

abstract：:Comparisons of the structures of glutamate dehydrogenase (GluDH) and leucine dehydrogenase (LeuDH) have suggested that two substitutions, deep within the amino acid binding pockets of these homologous enzymes, from hydrophilic residues to hydrophobic ones are critical components of their differential substrate specifi...

journal_title：Journal of molecular biology

authors： Stillman TJ,Migueis AM,Wang XG,Baker PJ,Britton KL,Engel PC,Rice DW

更新日期：1999-01-15 00:00:00

abstract：:Low molecular weight protein tyrosine phosphatase (LMW-PTP) dimerizes in the phosphate-bound state in solution with a dissociation constant of K(d)=1.5(+/-0.1)mM and an off-rate on the order of 10(4)s(-1). 1H and 15N NMR chemical shifts identify the dimer interface, which is in excellent agreement with that observed i...

journal_title：Journal of molecular biology

authors： Akerud T,Thulin E,Van Etten RL,Akke M

更新日期：2002-09-06 00:00:00

abstract：:Genetic studies in Saccharomyces cerevisiae predict that the mismatch repair (MMR) factor MSH2-MSH3 binds and stabilizes branched recombination intermediates that form during single strand annealing and gene conversion. To test this model, we constructed a series of DNA substrates that are predicted to form during the...

journal_title：Journal of molecular biology

authors： Surtees JA,Alani E

更新日期：2006-07-14 00:00:00

abstract：:Filamentous bacteriophage (Inovirus) is a simple and well-characterized model system. The phage particle, or virion, is about 60 angstroms in diameter and several thousand angstrom units long. The virions are assembled at the bacterial membrane as they extrude out of the host without killing it, an example of specific...

journal_title：Journal of molecular biology

authors： Marvin DA,Welsh LC,Symmons MF,Scott WR,Straus SK

更新日期：2006-01-13 00:00:00

abstract：:Transcription of the ribosomal RNA operons (rrn) in Escherichia coli is subject to an antitermination mechanism whereby RNA polymerase is modified to a termination-resistant form during transit through the rrn leader region. This antitermination mechanism is unable to overcome the T1-T2 terminator cluster located at t...

journal_title：Journal of molecular biology

authors： Ghosh B,Grzadzielska E,Bhattacharya P,Peralta E,DeVito J,Das A

更新日期：1991-11-05 00:00:00

abstract：:A complex pseudoknot structure surrounds the first ribosome initiation site in the Escherichia coli alpha mRNA and mediates its regulation by ribosomal protein S4. A 112 nt RNA fragment containing this pseudoknot exists in two conformations that are resolvable by gel electrophoresis below room temperature. Between 30 ...

journal_title：Journal of molecular biology

authors： Gluick TC,Gerstner RB,Draper DE

更新日期：1997-07-18 00:00:00

abstract：:A map of the 30 S ribosomal subunit is presented giving the positions of 15 of its 21 proteins. The components located in the map are S1, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S14, S15, S18 and S20. ...

journal_title：Journal of molecular biology

authors： Ramakrishnan V,Capel M,Kjeldgaard M,Engelman DM,Moore PB

更新日期：1984-04-05 00:00:00

abstract：:The NtrC-family PhhR protein of Pseudomonas putida is involved in the control of the metabolism of aromatic amino acids, and it is a dual regulatory protein. When PhhR acts as an activator, it stimulates transcription from its cognate promoters with RNA polymerase/sigma(70) rather than with sigma(54), as is the case f...

journal_title：Journal of molecular biology

authors： Herrera MC,Krell T,Zhang X,Ramos JL

更新日期：2009-12-04 00:00:00

abstract：:Vascular smooth muscle cell hypertrophy and proliferation may participate in the pathophysiology of cardiovascular disease. The analysis of changes in gene expression in vascular smooth muscle cells is crucial to the understanding of the molecular biology of cardiovascular disease. An effective method for analysis of ...

journal_title：Journal of molecular biology

authors： Kettenhofen R,Meyer zu Brickwedde MK,KO Y,Vetter H,Sachinidis A

更新日期：1998-06-05 00:00:00

abstract：:The primary obstacle to de novo protein structure prediction is conformational sampling: the native state generally has lower free energy than nonnative structures but is exceedingly difficult to locate. Structure predictions with atomic level accuracy have been made for small proteins using the Rosetta structure pred...

journal_title：Journal of molecular biology

authors： Kim DE,Blum B,Bradley P,Baker D

更新日期：2009-10-16 00:00:00

abstract：:In order to utilize 19F nuclear magnetic resonance (NMR) to probe the solution structure of Escherichia coli tRNAVal labeled by incorporation of 5-fluorouracil, we have assigned its 19F spectrum. We describe here assignments made by examining the spectra of a series of tRNAVal mutants with nucleotide substitutions for...

journal_title：Journal of molecular biology

authors： Chu WC,Feiz V,Derrick WB,Horowitz J

更新日期：1992-10-20 00:00:00

abstract：:Phenylalanine hydroxylase from rat liver has been crystallized from polyethylene glycol 4500 with sodium formate. The crystals are tetragonal rods and belong to space group P4(1)22 or P4(3)22 with unit cell dimensions a = b = 57.6 A and c = 304.1 A. They diffract to at least 2.4 A resolution and have one molecule per ...

journal_title：Journal of molecular biology

authors： Celikel R,Davis MD,Dai XP,Kaufman S,Xuong NH

更新日期：1991-04-05 00:00:00

abstract：:The 3' terminus of the (-) RNA strand in the replicative forms of several (+)-stranded RNA viruses possesses an unpaired guanosine with unknown function. This unpaired guanosine is also found at the 3' terminus of the (-) strand in the double-stranded form of two cucumoviral satellite RNAs. Using a cucumber mosaic vir...

journal_title：Journal of molecular biology

authors： Wu G,Kaper JM

更新日期：1994-05-20 00:00:00

abstract：:The importance of including different energy contributions in calculations of electrostatic energies in proteins is examined by calculating the intrinsic pKa values of the acidic groups of bovine pancreatic trypsin inhibitor. It appears that such calculations provide a powerful and revealing test; the relevant solvati...

journal_title：Journal of molecular biology

authors： Russell ST,Warshel A

更新日期：1985-09-20 00:00:00

abstract：:The cyclin-dependent kinase Cdk1 and the related kinase Ime2 act in concert to trigger progression of the meiotic cell cycle in the yeast Saccharomyces cerevisiae. These kinases share several functions and substrates during meiosis, but their regulation seems to be clearly different. In contrast to Cdk1, no cyclin see...

journal_title：Journal of molecular biology

authors： Sari F,Heinrich M,Meyer W,Braus GH,Irniger S

更新日期：2008-04-18 00:00:00

abstract：:Using a human non-immune phage library comprising more than 10(9) functional human antibody specificities in Fab format, we have been able to select a set of eight monoclonal Fabs targeted against diverse epitopes of the ectodomain of gp41 from HIV-1. The antigens used for panning the antibodies comprised two soluble,...

journal_title：Journal of molecular biology

authors： Louis JM,Bewley CA,Gustchina E,Aniana A,Clore GM

更新日期：2005-11-11 00:00:00

abstract：:The enthalpy of hydration of polar and non-polar groups upon protein unfolding has been estimated for four globular proteins in the temperature range 5 to 125 degrees C, using structural information on the groups in these proteins exposed to water in the native and unfolded states and volume-corrected calorimetric inf...

journal_title：Journal of molecular biology

authors： Makhatadze GI,Privalov PL

更新日期：1993-07-20 00:00:00

abstract：:We describe a hidden Markov model, HMMSTR, for general protein sequence based on the I-sites library of sequence-structure motifs. Unlike the linear hidden Markov models used to model individual protein families, HMMSTR has a highly branched topology and captures recurrent local features of protein sequences and struc...

journal_title：Journal of molecular biology

authors： Bystroff C,Thorsson V,Baker D

更新日期：2000-08-04 00:00:00

abstract：:Mitochondrial function depends on the correct synthesis, transport, and assembly of proteins and cofactors of the electron transport chain. The initial idea that the respiratory chain protein complexes (RCCs) were independent structures in the inner mitochondrial membrane evolved after the identification of higher qua...

journal_title：Journal of molecular biology

authors： Cogliati S,Lorenzi I,Rigoni G,Caicci F,Soriano ME

更新日期：2018-12-07 00:00:00

abstract：:The Escherichia coli dnaA gene is required for replication of the bacterial chromosome. To identify residues critical for its replication activity, a method to select novel mutations was developed that relied on lytic growth of lambda from an inserted pSC101 replication origin. Replication from the lambda origin was i...

journal_title：Journal of molecular biology

authors： Sutton MD,Kaguni JM

更新日期：1997-09-05 00:00:00

abstract：:It has been suggested that modified nucleosides of the xm5(s2)U(m)34-type restrict the wobble capacity of the base, and that their function is to prevent misreading in the third position of the codon in mixed codon family boxes that encode two different amino acids. In this study in Escherichia coli, the misreading in...

journal_title：Journal of molecular biology

authors： Hagervall TG,Pomerantz SC,McCloskey JA

更新日期：1998-11-20 00:00:00

abstract：:We have studied the conditions needed for the formation of stable ternary complexes by Escherichia coli RNA polymerase using a procedure in which elongation by the majority of active enzyme molecules is halted at a specific template base. Stable complexes of this sort, containing RNA chains as short as 15 nucleotides,...

journal_title：Journal of molecular biology

authors： Levin JR,Krummel B,Chamberlin MJ

更新日期：1987-07-05 00:00:00

abstract：:A number of beta-sandwich immunoglobulin-like domains have been shown to fold using a set of structurally equivalent residues that form a folding nucleus deep within the core of the protein. Formation of this nucleus is sufficient to establish the complex Greek key topology of the native state. These nucleating residu...

journal_title：Journal of molecular biology

authors： Lappalainen I,Hurley MG,Clarke J

更新日期：2008-01-11 00:00:00

abstract：:Successful immunotherapy of cocaine addiction and overdoses requires cocaine-binding antibodies with specific properties, such as high affinity and selectivity for cocaine. We have determined the affinities of two cocaine-binding murine monoclonal antibodies (mAb: clones 3P1A6 and MM0240PA) for cocaine and its metabol...

journal_title：Journal of molecular biology

authors： Paula S,Tabet MR,Keenan SM,Welsh WJ,Ball WJ Jr

更新日期：2003-01-17 00:00:00

abstract：:Iron-sulfur (Fe/S)-cluster-containing proteins constitute one of the largest protein classes, with varied functions that include electron transport, regulation of gene expression, substrate binding and activation, and radical generation. Consequently, the biosynthetic machinery for Fe/S clusters is evolutionarily cons...

journal_title：Journal of molecular biology

authors： Wachnowsky C,Wesley NA,Fidai I,Cowan JA

更新日期：2017-03-24 00:00:00

abstract：:Azaleucine is a naturally occurring amino acid antibiotic that can be incorporated into proteins by mimicking leucine. In spite of its highly toxic character, the contrast between the hydrophobic side-chain of leucine and the basic side-chain of azaleucine suggested a mechanism for rescuing certain mutants. We constru...

journal_title：Journal of molecular biology

authors： Lemeignan B,Sonigo P,Marlière P

更新日期：1993-05-20 00:00:00

abstract：:Eosinophil cationic protein (ECP; RNase 3) is a human ribonuclease found only in eosinophil leukocytes that belongs to the RNase A superfamily. This enzyme is bactericidal, helminthotoxic and cytotoxic to mammalian cells and tissues. The protein has been cloned, heterologously overexpressed, purified and crystallized....

journal_title：Journal of molecular biology

authors： Mallorquí-Fernández G,Pous J,Peracaula R,Aymamí J,Maeda T,Tada H,Yamada H,Seno M,de Llorens R,Gomis-Rüth FX,Coll M

更新日期：2000-07-28 00:00:00

abstract：:Catalytic antibodies 6D9 and 9C10, which were induced by immunization with a haptenic transition-state analog (TSA), catalyze the hydrolysis of a nonbioactive chloramphenicol monoester derivative to generate a bioactive chloramphenicol. These antibodies stabilize the transition state to catalyze the hydrolysis reactio...

journal_title：Journal of molecular biology

authors： Oda M,Ito N,Tsumuraya T,Suzuki K,Sakakura M,Fujii I

更新日期：2007-05-25 00:00:00

abstract：:The X-ray structure of the GCN4-bZIP protein bound to DNA containing the ATF/CREB recognition sequence has been refined at 2.2 A. The water-mediated interactions between the basic domain and DNA are revealed, and combined with a more accurate description of the direct contacts, further clarify how binding specificity ...

journal_title：Journal of molecular biology

authors： Keller W,König P,Richmond TJ

更新日期：1995-12-08 00:00:00

abstract：:DDX3X is a conserved DEAD-box RNA helicase involved in translation initiation and other processes of RNA metabolism. Mutations in human DDX3X and deregulation of its expression are linked to tumorigenesis and intellectual disability. The protein is also targeted by diverse viruses. Previous studies demonstrated helica...

journal_title：Journal of molecular biology

authors： Sharma D,Putnam AA,Jankowsky E

更新日期：2017-11-24 00:00:00