Plasticity within the obligatory folding nucleus of an immunoglobulin-like domain.

abstract：:Using HU chemical nucleases to probe HU-DNA interactions, we report here for the first time site-specific binding of HU to naked DNA. An unique feature of this interaction is the absolute requirement for negative DNA supercoiling for detectable levels of site-specific DNA binding. The HU binding site is the Mu spacer ...

journal_title：Journal of molecular biology

authors： Kobryn K,Lavoie BD,Chaconas G

更新日期：1999-06-18 00:00:00

abstract：:The maturation of proteases is governed by prosequences. During the biogenesis of the highly oligomeric eukaryotic 20 S proteasome five different prosequence-containing subunits have to be integrated and processed either by autocatalysis or by neighbouring subunits. To analyse the functional impact of proteasomal pros...

journal_title：Journal of molecular biology

authors： Schmidt M,Zantopf D,Kraft R,Kostka S,Preissner R,Kloetzel PM

更新日期：1999-04-23 00:00:00

abstract：:The seeds of jack fruit (Artocarpus integrifolia) contain two tetrameric lectins, jacalin and artocarpin. Jacalin was the first lectin found to exhibit the beta-prism I fold, which is characteristic of the Moraceae plant lectin family. Jacalin contains two polypeptide chains produced by a post-translational proteolysi...

journal_title：Journal of molecular biology

authors： Pratap JV,Jeyaprakash AA,Rani PG,Sekar K,Surolia A,Vijayan M

更新日期：2002-03-22 00:00:00

abstract：:The humoral immune system in higher vertebrates is unique in its ability to generate highly diverse antibody responses against most pathogens as well as against certain malignancies. Several technologies have been developed to exploit this vast source of potentially therapeutic antibodies, including hybridoma technolo...

journal_title：Journal of molecular biology

authors： Meijer PJ,Andersen PS,Haahr Hansen M,Steinaa L,Jensen A,Lantto J,Oleksiewicz MB,Tengbjerg K,Poulsen TR,Coljee VW,Bregenholt S,Haurum JS,Nielsen LS

更新日期：2006-05-05 00:00:00

abstract：:Archaebacterial respiratory chains are poorly understood at the molecular level. We have cloned and sequenced a cluster of five new genes from the archaebacterium Sulfolobus acidocaldarius, four of them coding for redox proteins: a Rieske iron-sulphur protein, a cytochrome b, a subunit II of cytochrome oxidase and a b...

journal_title：Journal of molecular biology

authors： Castresana J,Lübben M,Saraste M

更新日期：1995-07-07 00:00:00

abstract：:By binding to the DNA site OE at position -60.5 in the gal operon, the GalR protein activates transcription from the P2 promoter located on the opposite face of DNA (position -5) and represses transcription from the P1 promoter located on the same face (position +1). GalR increases RNA polymerase binding at P2 and inh...

journal_title：Journal of molecular biology

authors： Choy HE,Hanger RR,Aki T,Mahoney M,Murakami K,Ishihama A,Adhya S

更新日期：1997-09-26 00:00:00

abstract：:The evolution of protein interactions cannot be deciphered without a detailed analysis of interaction interfaces and binding modes. We performed a large-scale study of protein homooligomers in terms of their symmetry, interface sizes, and conservation of binding modes. We also focused specifically on the evolution of ...

journal_title：Journal of molecular biology

authors： Dayhoff JE,Shoemaker BA,Bryant SH,Panchenko AR

更新日期：2010-01-29 00:00:00

abstract：:Escherichia coli PriA is a primosome assembly protein with 3' to 5' helicase activity whose apparent function is to promote resumption of DNA synthesis following replication-fork arrest. Here, we describe how initiation of helicase activity on DNA forks is influenced by both fork structure and by single-strand DNA-bin...

journal_title：Journal of molecular biology

authors： Jones JM,Nakai H

更新日期：2001-10-05 00:00:00

abstract：:Nebulin, a large protein (600 to 800 kDa) located in the thin filament of striated vertebrate muscle, is assumed to bind and stabilise F-actin. Complete sequence determination of human nebulin has only recently been accomplished showing a uniform modular structure along the whole length of the molecule. Up to 97% of t...

journal_title：Journal of molecular biology

authors： Pfuhl M,Winder SJ,Castiglione Morelli MA,Labeit S,Pastore A

更新日期：1996-03-29 00:00:00

abstract：:This study examined the genomic organisation of the coding region of the glutathione S-transferase 3 (Ov-GST-3) from the human parasitic nematode Onchocerca volvulus; alternative splicing leads to three different transcripts (Ov-GST-3/1; Ov-GST-3/2 and Ov-GST-3/3). Since the expression of Ov-GST-3 is inducible by oxid...

journal_title：Journal of molecular biology

authors： Kampkötter A,Volkmann TE,de Castro SH,Leiers B,Klotz LO,Johnson TE,Link CD,Henkle-Dührsen K

更新日期：2003-01-03 00:00:00

abstract：:The bacterial flagellar motor is a rotary motor in the cell envelope of bacteria that couples ion flow across the cytoplasmic membrane to torque generation by independent stators anchored to the cell wall. The recent observation of stepwise rotation of a Na(+)-driven chimeric motor in Escherichia coli promises to reve...

journal_title：Journal of molecular biology

authors： Inoue Y,Lo CJ,Fukuoka H,Takahashi H,Sowa Y,Pilizota T,Wadhams GH,Homma M,Berry RM,Ishijima A

更新日期：2008-03-07 00:00:00

abstract：:We demonstrate here that the Saccharomyces cerevisiae Mlh1-Pms1 heterodimer required for DNA mismatch repair and other cellular processes is a DNA binding protein. Binding was evaluated using a variety of single and double-stranded DNA molecules. Mlh1-Pms1 bound short substrates with low affinity and showed a slight p...

journal_title：Journal of molecular biology

authors： Hall MC,Wang H,Erie DA,Kunkel TA

更新日期：2001-09-28 00:00:00

abstract：:Small RNAs were selected from a highly degenerate library on the basis of their ability to bind tightly to Escherichia coli phenylalanyl-tRNA synthetase (FRS). The 63 nucleotide library consisted of the acceptor stem and portions of the D and T stems of E. coli tRNA(Phe) flanking a 32 nucleotide randomized region. Bec...

journal_title：Journal of molecular biology

authors： Peterson ET,Pan T,Coleman J,Uhlenbeck OC

更新日期：1994-09-23 00:00:00

abstract：:The nuclear receptor pregnane X receptor (PXR) acts as a sensor for a broad variety of natural and synthetic lipophilic compounds, such as bile acids and rifampicin, and regulates the expression of proteins that are involved in the metabolism and transport of these compounds. PXR binds as a heterodimer with the retino...

journal_title：Journal of molecular biology

authors： Frank C,Makkonen H,Dunlop TW,Matilainen M,Väisänen S,Carlberg C

更新日期：2005-02-18 00:00:00

abstract：:Shikimate kinase (SK) and other enzymes in the shikimate pathway are potential targets for developing non-toxic antimicrobial agents, herbicides, and anti-parasite drugs, because the pathway is essential in the above species but is absent from mammals. The crystal structure of Mycobacterium tuberculosis SK (MtSK) in c...

journal_title：Journal of molecular biology

authors： Gu Y,Reshetnikova L,Li Y,Wu Y,Yan H,Singh S,Ji X

更新日期：2002-06-07 00:00:00

abstract：:Crystals of NADPH:FMN oxidoreductase from Vibrio harveyi have been obtained and characterized by X-ray diffraction. This enzyme plays a role in the generation of light in luminescent bacteria by providing reduced FMN to luciferase. Large, high quality crystals were grown using polyethylene glycol 6000 at pH 7.0. They ...

journal_title：Journal of molecular biology

authors： Tanner J,Lei B,Liu M,Tu SC,Krause KL

更新日期：1994-08-12 00:00:00

abstract：:Homotypic death domain (DD)-DD interactions are important in the assembly of oligomeric signaling complexes such as the PIDDosome that acts as a platform for activation of caspase-2-dependent apoptotic signaling. The structure of the PIDDosome core complex exhibits an asymmetric three-layered arrangement containing fi...

journal_title：Journal of molecular biology

authors： Nematollahi LA,Garza-Garcia A,Bechara C,Esposito D,Morgner N,Robinson CV,Driscoll PC

更新日期：2015-02-27 00:00:00

abstract：:Aquaporin-1 (AQP1) is an abundant protein in human erythrocyte membranes which functions as a specific and constitutively active water conducting pore. Solubilized and isolated as tetramer, it forms well-ordered two-dimensional (2D) crystals when reconstituted in the presence of lipids. Several high resolution project...

journal_title：Journal of molecular biology

authors： Walz T,Tittmann P,Fuchs KH,Müller DJ,Smith BL,Agre P,Gross H,Engel A

更新日期：1996-12-20 00:00:00

abstract：:Missense mutations of the p53 tumour suppressor gene induce the formation of proteins with an altered affinity for DNA. These mutant proteins have either a wild-type or a mutant conformation. It has been established that, on association with wild-type protein, molecules with mutant conformation can drive the wild-type...

journal_title：Journal of molecular biology

authors： Chène P

更新日期：1998-08-14 00:00:00

abstract：:The hepatitis delta virus (HDV) ribozyme is a self-cleaving RNA that resides in the HDV genome and regulates its replication. The native fold of the ribozyme is complex, having two pseudoknots. Earlier work implicated four non-native pairings in slowing pseudoknot formation: Alt 1, Alt 2, Alt 3, and Alt P1. The goal o...

journal_title：Journal of molecular biology

authors： Brown TS,Chadalavada DM,Bevilacqua PC

更新日期：2004-08-13 00:00:00

abstract：:The nature of the nucleation-collapse mechanism in protein folding is probed using 27-mer and 36-mer lattice models. Three different forms for the interaction potentials are used. Three of the four 27-mer sequences have maximally compact and identical native state while the other has a non-compact native conformation....

journal_title：Journal of molecular biology

authors： Klimov DK,Thirumalai D

更新日期：1998-09-18 00:00:00

abstract：:The four approximately 75-residue domains (repeats) that constitute the annexin core structure all possess an identical five-alpha-helix bundle topology, but the physico-chemical properties of the isolated domains are different. Domain IV of the annexins has previously been expressed only as inclusion bodies, resistan...

journal_title：Journal of molecular biology

authors： Aukrust I,Evensen L,Hollås H,Berven F,Atkinson RA,Travé G,Flatmark T,Vedeler A

更新日期：2006-10-20 00:00:00

abstract：:Many gram-negative bacteria that are important human pathogens possess type III secretion systems as part of their required virulence factor repertoire. During the establishment of infection, these pathogens coordinately assemble greater than 20 different proteins into a macromolecular structure that spans the bacteri...

journal_title：Journal of molecular biology

authors： Zhang L,Wang Y,Picking WL,Picking WD,De Guzman RN

更新日期：2006-06-02 00:00:00

abstract：:Centrin, an EF-hand calcium-binding protein, has been shown to be involved in the duplication of centrosomes, and Sfi1 (Suppressor of fermentation-induced loss of stress resistance protein 1) is one of its centrosomal targets. There are three isoforms of human centrin, but here we only considered centrin 2 (HsCen2). T...

journal_title：Journal of molecular biology

authors： Martinez-Sanz J,Kateb F,Assairi L,Blouquit Y,Bodenhausen G,Abergel D,Mouawad L,Craescu CT

更新日期：2010-01-08 00:00:00

abstract：:The nucleotide sequence of 6.2 kb (1 kb = 10(3) base-pairs) of DNA that encompasses the earliest replicating portion of the amplified dihydrofolate reductase domains of CHOC 400 cells has been determined. Origin region DNA contains two AluI family repeats, a novel repetitive element (termed ORR-1), a TGGGT-rich region...

journal_title：Journal of molecular biology

authors： Caddle MS,Lussier RH,Heintz NH

更新日期：1990-01-05 00:00:00

abstract：:Repeat proteins are formed from units of 20-40 aa that stack together into quasi one-dimensional non-globular structures. This modular repetitive construction means that, unlike globular proteins, a repeat protein's equilibrium folding and thus thermodynamic stability can be analysed using linear Ising models. Typical...

journal_title：Journal of molecular biology

authors： Millership C,Phillips JJ,Main ER

更新日期：2016-05-08 00:00:00

abstract：:Porins, such as Escherichia coli OmpF, provide the only reported example of a voltage-gated channel where the three-dimensional structure is known to high resolution. Mutations that affect voltage-gating are clustered around the eyelet region, which is a mid-channel constriction caused by a polypeptide loop (L3) foldi...

journal_title：Journal of molecular biology

authors： Bainbridge G,Mobasheri H,Armstrong GA,Lea EJ,Lakey JH

更新日期：1998-01-16 00:00:00

abstract：:The amino acid sequence of the rod portion of talin contains strong periodic patterns with a long period of 32 to 34 residues superimposed on short periods of 7 and 7/2 residues. The rod includes 50 to 60 copies of an irregular repeated motif approximately 34 residues long. The motif itself consists of three sections:...

journal_title：Journal of molecular biology

authors： McLachlan AD,Stewart M,Hynes RO,Rees DJ

更新日期：1994-01-28 00:00:00

abstract：:We have performed thin-section electron microscopy on muscle fibers fixed in different mechanically monitored states, in order to identify structural changes in myosin crossbridges associated with force production and maintenance. Tension and stiffness of fibers from glycerinated Lethocerus flight muscle were monitore...

journal_title：Journal of molecular biology

authors： Reedy MC,Reedy MK,Tregear RT

更新日期：1988-11-20 00:00:00

abstract：:A key feature of transcriptional attenuation in some amino acid biosynthetic operons is a transcriptional pause that occurs immediately after synthesis of the first leader transcript secondary structure. Both RNA secondary structure and downstream DNA sequence are important for pausing at these sites; however, the pre...

journal_title：Journal of molecular biology

authors： Chan CL,Landick R

更新日期：1993-09-05 00:00:00