In vitro analysis of the dominant negative effect of p53 mutants.

abstract：:Nudaurelia capensis omega virus is a T=4, icosahedral virus with a bipartite, positive-sense RNA genome. Expression of the coat protein gene in a baculovirus system was previously shown to result in the formation of procapsids when purified at pH 7.6. Procapsids are round, porous particles (480 A diameter) and have T=...

journal_title：Journal of molecular biology

authors： Tang J,Lee KK,Bothner B,Baker TS,Yeager M,Johnson JE

更新日期：2009-09-25 00:00:00

abstract：:The formation of membraneless organelles (MLOs) by phase separation has emerged as a new way of organizing the cytoplasm and nucleoplasm of cells. Examples of MLOs forming via phase separation are nucleoli in the nucleus and stress granules in the cytoplasm. The main components of these MLOs are macromolecules such as...

journal_title：Journal of molecular biology

authors： Alberti S,Carra S

更新日期：2018-11-02 00:00:00

abstract：:Physarum polycephalum hemagglutinin I (HA1) is a 104-residue protein that is secreted to extracellular space. The crystal structure of HA1 has a β-sandwich fold found among lectin structures, such as legume lectins and galectins. Interestingly, the β-sandwich of HA1 lacks a jelly roll motif and is essentially composed...

journal_title：Journal of molecular biology

authors： Kouno T,Watanabe N,Sakai N,Nakamura T,Nabeshima Y,Morita M,Mizuguchi M,Aizawa T,Demura M,Imanaka T,Tanaka I,Kawano K

更新日期：2011-01-14 00:00:00

abstract：:The thermal melting of a dicyclic 29-residue peptide, having helix-stabilizing side-chain to side-chain covalent links at each terminal, has been studied by circular dichroism spectropolarimetry (CD) and differential scanning calorimetry (DSC). The CD spectra for this dicyclic peptide indicate that it is monomeric, al...

journal_title：Journal of molecular biology

authors： Taylor JW,Greenfield NJ,Wu B,Privalov PL

更新日期：1999-08-27 00:00:00

abstract：:Analysis of the conformational differences between the oxy and deoxy forms of hemoglobin is complicated by shifting coordinate systems and correlated motions between different parts of the molecule. Methods independent of any frame of reference were used to study the differences in structure between the oxy and deoxy ...

journal_title：Journal of molecular biology

authors： Nichols WL,Zimm BH,Ten Eyck LF

更新日期：1997-07-25 00:00:00

abstract：:We have probed the surface accessibility of residues alpha187 to alpha199 of the Torpedo acetylcholine receptor with monoclonal antibody 383C, which binds uniquely to these residues. However, 383C binds to only one of the two alpha subunits in the membrane-bound receptor, neither of the two subunits in carbamylcholine...

journal_title：Journal of molecular biology

authors： Fairclough RH,Twaddle GM,Gudipati E,Lin MY,Richman DP

更新日期：1998-09-18 00:00:00

abstract：:Cyanobacteria are shown to be unique in containing membrane-bound manganese superoxide dismutases (MnSOD). They are homodimeric type 2 membrane proteins that protect this phototrophic organism against oxidative stress. We have determined, for the first time, the 2.0A resolution structure of the catalytic portion of th...

journal_title：Journal of molecular biology

authors： Atzenhofer W,Regelsberger G,Jacob U,Peschek G,Furtmüller P,Huber R,Obinger C

更新日期：2002-08-16 00:00:00

abstract：:The structure and backbone dynamics of a double labelled (15N,13C) monomeric, 23.7 kD phosphoglycerate mutase (PGAM) from Schizosaccharomyces pombe have been investigated in solution using NMR spectroscopy. A set of 3125 NOE-derived distance restraints, 148 restraints representing inferred hydrogen bonds and 149 value...

journal_title：Journal of molecular biology

authors： Uhrínová S,Uhrín D,Nairn J,Price NC,Fothergill-Gilmore LA,Barlow PN

更新日期：2001-02-16 00:00:00

abstract：:We have isolated cytoplasmic ribosomes from Euglena gracilis and characterized the RNA components of these particles. We show here that instead of the four rRNAs (17-19 S, 25-28 S, 5.8 S and 5 S) found in typical eukaryotic ribosomes, Euglena cytoplasmic ribosomes contain 16 RNA components. Three of these Euglena rRNA...

journal_title：Journal of molecular biology

authors： Schnare MN,Gray MW

更新日期：1990-09-05 00:00:00

abstract：:Staphylococcal enterotoxins and toxic shock syndrome toxin-1 are known as superantigens due to their ability to activate a large number of T-cells by crosslinking the major histocompatibility complex class II molecules with the T-cell receptor. Although superantigens seem to act by a common mechanism, they vary in man...

journal_title：Journal of molecular biology

authors： Schad EM,Papageorgiou AC,Svensson LA,Acharya KR

更新日期：1997-06-06 00:00:00

abstract：:The structural fluctuations specific to self-assembled biological systems have been investigated further with ultrasonic techniques by using two strains of tobacco mosaic virus (TMV), as well as the helical aggregate of the common strain protein and subassemblies of it. We confirmed our earlier conclusion that protein...

journal_title：Journal of molecular biology

authors： Michels B,Dormoy Y,Cerf R,Schulz JA,Witz J

更新日期：1985-01-05 00:00:00

abstract：:The crystal structure of human sex hormone-binding globulin (SHBG) has revealed how 5alpha-dihydrotestosterone intercalates between the two seven-stranded beta-sheets of its amino-terminal laminin G-like domain. However, a region of disorder (residues 130 to 135 of SHBG) was identified together with a zinc-binding sit...

journal_title：Journal of molecular biology

authors： Grishkovskaya I,Avvakumov GV,Hammond GL,Muller YA

更新日期：2002-05-03 00:00:00

abstract：:The structure of replicating simian virus 40 (SV40) minichromosomes was studied by DNA crosslinking with trimethyl-psoralen. The procedure was used both in vitro with extracted SV40 minichromosomes as well as in vivo with SV40-infected cells. Both procedures gave essentially the same results. Mature SV40 minichromosom...

journal_title：Journal of molecular biology

authors： Sogo JM,Stahl H,Koller T,Knippers R

更新日期：1986-05-05 00:00:00

abstract：:The crystal structures of the nucleotide-empty (A(E)), 5'-adenylyl-beta,gamma-imidodiphosphate (A(PNP))-bound, and ADP (A(DP))-bound forms of the catalytic A subunit of the energy producer A(1)A(O) ATP synthase from Pyrococcus horikoshii OT3 have been solved at 2.47 A and 2.4 A resolutions. The structures provide nove...

journal_title：Journal of molecular biology

authors： Kumar A,Manimekalai MS,Balakrishna AM,Jeyakanthan J,Grüber G

更新日期：2010-02-19 00:00:00

abstract：:A number of beta-sandwich immunoglobulin-like domains have been shown to fold using a set of structurally equivalent residues that form a folding nucleus deep within the core of the protein. Formation of this nucleus is sufficient to establish the complex Greek key topology of the native state. These nucleating residu...

journal_title：Journal of molecular biology

authors： Lappalainen I,Hurley MG,Clarke J

更新日期：2008-01-11 00:00:00

abstract：:The projected structure of the vanadate-induced crystalline aggregates of Ca2+-ATPase molecules in isolated sarcoplasmic reticulum membranes has been determined. The molecules form tubular crystals with an oblique surface lattice having cell dimensions a = 65.9 A, b = 114.4 A and gamma = 77.9 degrees. The space group ...

journal_title：Journal of molecular biology

authors： Taylor K,Dux L,Martonosi A

更新日期：1984-03-25 00:00:00

abstract：:The recombination directionality factor, Xis, is a DNA bending protein that determines the outcome of integrase-mediated site-specific recombination by redesign of higher-order protein-DNA architectures. Although the attachment site DNA of mycobacteriophage Pukovnik is likely to contain four sites for Xis binding, Xis...

journal_title：Journal of molecular biology

authors： Singh S,Plaks JG,Homa NJ,Amrich CG,Héroux A,Hatfull GF,VanDemark AP

更新日期：2014-01-23 00:00:00

abstract：:The ability to form selective cell-cell adhesions is an essential property of metazoan cells. Members of the cadherin superfamily are important regulators of this process in both vertebrates and invertebrates. With the advent of genome sequencing projects, determination of the full repertoire of cadherins available to...

journal_title：Journal of molecular biology

authors： Hill E,Broadbent ID,Chothia C,Pettitt J

更新日期：2001-02-02 00:00:00

abstract：:The three-dimensional structures of engineered variants of Bacillus lentus subtilisin having increased enzymatic activity, K27R/N87S/V104Y/N123S/T274A (RSYSA) and N76D/N87S/S103A/V104I (DSAI), were determined by X-ray crystallography. In addition to identifying changes in atomic position we report a method that identi...

journal_title：Journal of molecular biology

authors： Graycar T,Knapp M,Ganshaw G,Dauberman J,Bott R

更新日期：1999-09-10 00:00:00

abstract：:The proteins coded by the five major capsid genes of herpes simplex virus 1, VP5 (gene UL19), VP19c (UL38), VP23 (UL18), pre-VP22a (UL26.5), and pre-VP21 (UL26), assemble into fragile roundish "procapsids", which mature into robust polyhedral capsids in a transition similar to that undergone by bacteriophage proheads....

journal_title：Journal of molecular biology

authors： Trus BL,Booy FP,Newcomb WW,Brown JC,Homa FL,Thomsen DR,Steven AC

更新日期：1996-11-01 00:00:00

abstract：:The exceptional versatility of calmodulin (CaM) three-dimensional arrangement is reflected in the growing number of structural models of CaM/protein complexes currently available in the Protein Data Bank (PDB) database, revealing a great diversity of conformations, domain organization, and structural responses to Ca(2...

journal_title：Journal of molecular biology

authors： Villarroel A,Taglialatela M,Bernardo-Seisdedos G,Alaimo A,Agirre J,Alberdi A,Gomis-Perez C,Soldovieri MV,Ambrosino P,Malo C,Areso P

更新日期：2014-07-29 00:00:00

abstract：:To investigate the relationships between sequence conservation, protein stability, and protein function, we have measured the thermodynamic stability, folding kinetics, and in vitro peptide-binding activity of a large number of single-site substitutions in the hydrophobic core of the Fyn SH3 domain. Comparison of thes...

journal_title：Journal of molecular biology

authors： Di Nardo AA,Larson SM,Davidson AR

更新日期：2003-10-24 00:00:00

abstract：:The methyltransferase RlmA(II) (formerly TlrB) is found in many Gram-positive bacteria, and methylates the N-1 position of nucleotide G748 within the loop of hairpin 35 in 23S rRNA. Methylation of the rRNA by RlmA(II) confers resistance to tylosin and other mycinosylated 16-membered ring macrolide antibiotics. We have...

journal_title：Journal of molecular biology

authors： Lebars I,Husson C,Yoshizawa S,Douthwaite S,Fourmy D

更新日期：2007-09-14 00:00:00

abstract：:A mutant of bovine pancreatic trypsin inhibitor (BPTI) has been constructed and expressed in Escherichia coli in order to probe the kinetic and structural consequences of truncating the binding loop residues to alanine. In addition to two such mutations (Thr11Ala and Pro13Ala), it has a conservative Lys15Arg substitut...

journal_title：Journal of molecular biology

authors： Czapinska H,Otlewski J,Krzywda S,Sheldrick GM,Jaskólski M

更新日期：2000-02-04 00:00:00

abstract：:The visible absorption spectra of carbonmonoxymyoglobin in the temperature range 300 to 20 K are reported and compared with the analogous spectra of carbonomonoxyhaemoglobin. The temperature dependence of the zeroth, first and second moment of the observed bands is analysed to obtain information on the local dynamics ...

journal_title：Journal of molecular biology

authors： Cordone L,Cupane A,Leone M,Vitrano E,Bulone D

更新日期：1988-01-05 00:00:00

abstract：:We report studies to determine which bacteriophage genes are required for assembly of phage HK97 proheads and what roles they play. We identify the gene encoding the major capsid protein of phage HK97 and report its DNA sequence, together with the DNA sequences of the two genes immediately upstream from it. When the c...

journal_title：Journal of molecular biology

authors： Duda RL,Martincic K,Hendrix RW

更新日期：1995-04-07 00:00:00

abstract：:In bacteria, the regulation of gene expression in response to changes in cell density is called quorum sensing. The autoinducer-2 production protein LuxS, is involved in a novel quorum-sensing system and is thought to catalyse the degradation of S-ribosylhomocysteine to homocysteine and the autoinducer molecule 4,5-di...

journal_title：Journal of molecular biology

authors： Ruzheinikov SN,Das SK,Sedelnikova SE,Hartley A,Foster SJ,Horsburgh MJ,Cox AG,McCleod CW,Mekhalfia A,Blackburn GM,Rice DW,Baker PJ

更新日期：2001-10-12 00:00:00

abstract：:Interactions among Bcl-2 family proteins are important for regulating apoptosis. Prosurvival members of the family interact with proapoptotic BH3 (Bcl-2-homology-3)-only members, inhibiting execution of cell death through the mitochondrial pathway. Structurally, this interaction is mediated by binding of the alpha-hel...

journal_title：Journal of molecular biology

authors： Dutta S,Gullá S,Chen TS,Fire E,Grant RA,Keating AE

更新日期：2010-05-21 00:00:00

abstract：:Binding of the TATA-binding protein (TBP) to the "TATA" sequences present in the promoters of eukaryotic class II genes is the first step in the sequential assembly of transcription pre-initiation complexes. Myriad structural changes, including severe bending of the DNA, accompany TBP-TATA complex formation. A detaile...

journal_title：Journal of molecular biology

authors： Parkhurst KM,Richards RM,Brenowitz M,Parkhurst LJ

更新日期：1999-06-25 00:00:00

abstract：:Telomeres are dynamic nucleoprotein structures that cap the ends of eukaryotic chromosomes. In humans, the long (TTAGGG)(n) double-stranded telomeric DNA repeats are bound specifically by the two related proteins TRF1 and TRF2, and are organized in nucleosomes. Whereas the role of TRF1 and TRF2 in telomeric function h...

journal_title：Journal of molecular biology

authors： Galati A,Rossetti L,Pisano S,Chapman L,Rhodes D,Savino M,Cacchione S

更新日期：2006-07-07 00:00:00