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Voltage-gating of Escherichia coli porin: a cystine-scanning mutagenesis study of loop 3.

Abstract：

:Porins, such as Escherichia coli OmpF, provide the only reported example of a voltage-gated channel where the three-dimensional structure is known to high resolution. Mutations that affect voltage-gating are clustered around the eyelet region, which is a mid-channel constriction caused by a polypeptide loop (L3) folding inside the lumen of this beta-barrel pore. These data, combined with molecular dynamics simulations, indicate that voltage-gating may involve L3 displacement. We have constructed six double cysteine OmpF mutants, five of which form disulphide bonds fixing L3 in the conformation determined by X-ray crystallography. These channels have altered single-channel conductances but unimpaired voltage-gating. The data show that L3 movement is not required for voltage-gating.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Bainbridge G,Mobasheri H,Armstrong GA,Lea EJ,Lakey JH

doi

10.1006/jmbi.1997.1474

subject

Has Abstract

pub_date

1998-01-16 00:00:00

pages

171-6

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(97)91474-1

journal_volume

275

pub_type

杂志文章

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