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Molecular evolution of multisubunit RNA polymerases: sequence analysis.

Abstract：

:Transcription in all cellular organisms is performed by multisubunit, DNA-dependent RNA polymerases that synthesize RNA from DNA templates. Previous sequence and structural studies have elucidated the importance of shared regions common to all multisubunit RNA polymerases. In addition, RNA polymerases contain multiple lineage-specific domain insertions involved in protein-protein and protein-nucleic acid interactions. We have created comprehensive multiple sequence alignments using all available sequence data for the multisubunit RNA polymerase large subunits, including the bacterial beta and beta' subunits and their homologs from archaebacterial RNA polymerases, the eukaryotic RNA polymerases I, II, and III, the nuclear-cytoplasmic large double-stranded DNA virus RNA polymerases, and plant plastid RNA polymerases. To overcome technical difficulties inherent to the large-subunit sequences, including large sequence length, small and large lineage-specific insertions, split subunits, and fused proteins, we created an automated and customizable sequence retrieval and processing system. In addition, we used our alignments to create a more expansive set of shared sequence regions and bacterial lineage-specific domain insertions. We also analyzed the intergenic gap between the bacterial beta and beta' genes.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Lane WJ,Darst SA

doi

10.1016/j.jmb.2009.10.062

subject

Has Abstract

pub_date

2010-01-29 00:00:00

pages

671-85

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(09)01321-7

journal_volume

395

pub_type

杂志文章

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