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Crystal structure of tryptophanase.

Abstract：

:The X-ray structure of tryptophanase (Tnase) reveals the interactions responsible for binding of the pyridoxal 5'-phosphate (PLP) and atomic details of the K+ binding site essential for catalysis. The structure of holo Tnase from Proteus vulgaris (space group P2(1)2(1)2(1) with a = 115.0 A, b = 118.2 A, c = 153.7 A) has been determined at 2.1 A resolution by molecular replacement using tyrosine phenol-lyase (TPL) coordinates. The final model of Tnase, refined to an R-factor of 18.7%, (Rfree = 22.8%) suggests that the PLP-enzyme from observed in the structure is a ketoenamine. PLP is bound in a cleft formed by both the small and large domains of one subunit and the large domain of the adjacent subunit in the so-called "catalytic" dimer. The K+ cations are located on the interface of the subunits in the dimer. The structure of the catalytic dimer and mode of PLP binding in Tnase resemble those found in aspartate amino-transferase, TPL, omega-amino acid pyruvate aminotransferase, dialkylglycine decarboxylase (DGD), cystathionine beta-lyase and ornithine decarboxylase. No structural similarity has been detected between Tnase and the beta 2 dimer of tryptophan synthase which catalyses the same beta-replacement reaction. The single monovalent cation binding site of Tnase is similar to that of TPL, but differs from either of those in DGD.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Isupov MN,Antson AA,Dodson EJ,Dodson GG,Dementieva IS,Zakomirdina LN,Wilson KS,Dauter Z,Lebedev AA,Harutyunyan EH

doi

10.1006/jmbi.1997.1561

subject

Has Abstract

pub_date

1998-02-27 00:00:00

pages

603-23

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(97)91561-8

journal_volume

276

pub_type

杂志文章

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