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Sigma competition: the contest between bacteriophage T4 middle and late transcription.

Abstract：

:In bacterial transcription, diverse sigma-family promoter recognition proteins compete for a common RNA polymerase core. Bacteriophage T4 infection ultimately reduces this competition to a duel between activated viral middle and enhanced late transcription, involving two sigma proteins, two phage-encoded activator proteins and two phage-specific co-activators. This competition has been analyzed in vitro, and the relative abundances in T4-infected Escherichia coli of the participating proteins have been measured. Activated late transcription holds an advantage over activated middle transcription, especially at higher ionic strength. This advantage is further compounded by ADP-ribosylation of the RNA polymerase alpha subunits, and by the phage-specific, RNA polymerase core-bound RpbA subunit. The largest contribution to the middle-late competition is made by gp55, the late sigma factor, but not enough of gp55 is produced during T4 infection to shut off middle transcription by direct competition with sigma(70). AsiA, the originally identified anti-sigma protein is not a major determinant of middle-late competition.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Kolesky S,Ouhammouch M,Brody EN,Geiduschek EP

doi

10.1006/jmbi.1999.2953

keywords:

subject

Has Abstract

pub_date

1999-08-13 00:00:00

pages

267-81

issue

2

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(99)92953-4

journal_volume

291

pub_type

杂志文章

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