当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Repression and activation of promoter-bound RNA polymerase activity by Gal repressor.

Repression and activation of promoter-bound RNA polymerase activity by Gal repressor.

Abstract：

:By binding to the DNA site OE at position -60.5 in the gal operon, the GalR protein activates transcription from the P2 promoter located on the opposite face of DNA (position -5) and represses transcription from the P1 promoter located on the same face (position +1). GalR increases RNA polymerase binding at P2 and inhibits isomerization at P1 by forming a GalR-DNA-RNA polymerase ternary complex in each case. The specific effect of GalR at one promoter is independent of the presence of the other promoter. The enhancement or repression is also not the intrinsic property of a promoter; the regulation can be reversed by switching the angular orientation of the promoters relative to OE. Both enhancement and repression appear to require the same interaction between RNA polymerase alpha-subunit and GalR and/or the same interaction between RNA polymerase alpha-subunit and DNA in the ternary complexes. We have discussed how GalR might exert opposite effects in the steps involved in the formation of the open complex from free RNA polymerase and DNA.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Choy HE,Hanger RR,Aki T,Mahoney M,Murakami K,Ishihama A,Adhya S

doi

10.1006/jmbi.1997.1221

subject

Has Abstract

pub_date

1997-09-26 00:00:00

pages

293-300

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(97)91221-3

journal_volume

272

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Cryo-electron microscopy of the giant Mimivirus.

    abstract：:Mimivirus is the largest known virus. Using cryo-electron microscopy, the virus was shown to be icosahedral, covered by long fibers, and appears to have at least two lipid membranes within its protein capsid. A unique vertex, presumably for attachment and infection of the host, can be seen for particles that have a su...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.08.060

    authors： Xiao C,Chipman PR,Battisti AJ,Bowman VD,Renesto P,Raoult D,Rossmann MG

    更新日期：2005-10-28 00:00:00

  • Structure of nucleosomal DNA at high salt concentration as probed by hydroxyl radical.

    abstract：:The structure of a 146 base-pair nucleosomal DNA has been probed using hydroxyl radical cleavage in buffers containing NaCl concentrations ranging from 80 mM to 800 mM. The highest salt concentrations used here are close to those required to dissociate core histone H2A and H2B from nucleosomal DNA. Nonetheless, the cl...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1089

    authors： Puhl HL,Behe MJ

    更新日期：1993-02-20 00:00:00

  • High-resolution crystal structures of Drosophila melanogaster angiotensin-converting enzyme in complex with novel inhibitors and antihypertensive drugs.

    abstract：:Angiotensin I-converting enzyme (ACE), one of the central components of the renin-angiotensin system, is a key therapeutic target for the treatment of hypertension and cardiovascular disorders. Human somatic ACE (sACE) has two homologous domains (N and C). The N- and C-domain catalytic sites have different activities ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.05.024

    authors： Akif M,Georgiadis D,Mahajan A,Dive V,Sturrock ED,Isaac RE,Acharya KR

    更新日期：2010-07-16 00:00:00

  • Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-D-mannose: implications to the generation of carbohydrate specificity.

    abstract：:The seeds of jack fruit (Artocarpus integrifolia) contain two tetrameric lectins, jacalin and artocarpin. Jacalin was the first lectin found to exhibit the beta-prism I fold, which is characteristic of the Moraceae plant lectin family. Jacalin contains two polypeptide chains produced by a post-translational proteolysi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5432

    authors： Pratap JV,Jeyaprakash AA,Rani PG,Sekar K,Surolia A,Vijayan M

    更新日期：2002-03-22 00:00:00

  • Structures of hepatitis B virus cores presenting a model epitope and their complexes with antibodies.

    abstract：:The core shell of hepatitis B virus is a potent immune stimulator, giving a strong neutralizing immune response to foreign epitopes inserted at the immunodominant region, located at the tips of spikes on the exterior of the shell. Here, we analyze structures of core shells with a model epitope inserted at two alternat...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.06.032

    authors： Roseman AM,Borschukova O,Berriman JA,Wynne SA,Pumpens P,Crowther RA

    更新日期：2012-10-12 00:00:00

  • Regulation of mating-type information in yeast. Negative control requiring sequences both 5' and 3' to the regulated region.

    abstract：:The genome of the yeast Saccharomyces cerevisiae contains three complete copies of the genetic information governing cell mating type. Normally, only the information in one of the copies (the MAT locus) is expressed; the other two copies (HML and HMR) are repressed and serve as donors of mating-type sequences that can...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90492-3

    authors： Abraham J,Nasmyth KA,Strathern JN,Klar AJ,Hicks JB

    更新日期：1984-07-05 00:00:00

  • Antisense-RNA mediated transcriptional attenuation: importance of a U-turn loop structure in the target RNA of plasmid pIP501 for efficient inhibition by the antisense RNA.

    abstract：:Antisense-RNA mediated gene regulation has been found and studied in detail mainly in prokaryotic accessory DNA elements. In spite of different regulatory mechanisms, in all cases a rapid interaction between antisense and target RNA has been shown to be crucial for efficient regulation. Recently, a sequence comparison...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2003.09.020

    authors： Heidrich N,Brantl S

    更新日期：2003-11-07 00:00:00

  • The dynamic structure of EF-G studied by fusidic acid resistance and internal revertants.

    abstract：:We have previously identified 20 different fusidic acid-resistant alleles of fusA, encoding mutant forms of the ribosomal translocase EF-G. One of these, P413L, is used here as the starting point in selections for internal revertants, identifying 20 different pseudo-wild-type forms of EF-G. We have also identified two...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0259

    authors： Johanson U,Aevarsson A,Liljas A,Hughes D

    更新日期：1996-05-10 00:00:00

  • Resolution of a disordered region at the entrance of the human sex hormone-binding globulin steroid-binding site.

    abstract：:The crystal structure of human sex hormone-binding globulin (SHBG) has revealed how 5alpha-dihydrotestosterone intercalates between the two seven-stranded beta-sheets of its amino-terminal laminin G-like domain. However, a region of disorder (residues 130 to 135 of SHBG) was identified together with a zinc-binding sit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00169-9

    authors： Grishkovskaya I,Avvakumov GV,Hammond GL,Muller YA

    更新日期：2002-05-03 00:00:00

  • Host-inhibitory functions encoded by promiscuous plasmids. Transient arrest of Escherichia coli segregants that fail to inherit plasmid RK2.

    abstract：:An effective strategy for a plasmid to be maintained in a growing population of bacteria is to prevent the proliferation of newly formed daughter cells that lack the plasmid. In this study, we determined that the promiscuous, incompatibility group P plasmid RK2 encodes such a system. To induce large numbers of RK2-fre...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1208

    authors： Jovanovic OS,Ayres EK,Figurski DH

    更新日期：1994-03-18 00:00:00

  • Antibody-antigen interactions: contact analysis and binding site topography.

    abstract：:We have analysed antigen-contacting residues and combining site shape in the antibody Fv and Fab crystal structures now available from the Protein Data Bank. Antigen-contacting propensities are presented for each antibody residue, allowing a new definition for the complementarity determining regions (CDRs) to be propo...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0548

    authors： MacCallum RM,Martin AC,Thornton JM

    更新日期：1996-10-11 00:00:00

  • Structural properties of a collagenous heterotrimer that mimics the collagenase cleavage site of collagen type I.

    abstract：:Collagens contain sequence- and conformation-dependent epitopes responsible for their digestion by collagenases at specific loci. A synthetic heterotrimer construct containing the collagenase cleavage site of collagen type I was found to mimic perfectly native collagen in terms of selectivity and mode of enzymatic deg...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00365-0

    authors： Fiori S,Saccà B,Moroder L

    更新日期：2002-06-21 00:00:00

  • Characterization of the dimer interface of transcription factor NFkappaB p50 homodimer.

    abstract：:Dimers of the Rel/NFkappaB transcription factor family form with differential stabilities through the combinatorial association of five polypeptides: p50, p52, p65, cRel, and RelB. Here, we have characterized the nature of the monomer-dimer equilibrium of the p50 homodimer. Sedimentation equilibrium studies show that ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2823

    authors： Sengchanthalangsy LL,Datta S,Huang DB,Anderson E,Braswell EH,Ghosh G

    更新日期：1999-06-18 00:00:00

  • Dynamic Properties of Human α-Synuclein Related to Propensity to Amyloid Fibril Formation.

    abstract：:α-Synuclein (αSyn) is an intrinsically disordered protein that can form amyloid fibrils. Fibrils of αSyn are implicated with the pathogenesis of Parkinson's disease and other synucleinopathies. Elucidating the mechanism of fibril formation of αSyn is therefore important for understanding the mechanism of the pathogene...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2019.05.047

    authors： Fujiwara S,Kono F,Matsuo T,Sugimoto Y,Matsumoto T,Narita A,Shibata K

    更新日期：2019-08-09 00:00:00

  • Structure-based identification of a ricin inhibitor.

    abstract：:Ricin is a potent cytotoxin which has been used widely in the construction of therapeutic agents such as immunotoxins. Recently it has been used by governments and underground groups as a poison. There is interest in identifying and designing effective inhibitors of the ricin A chain (RTA). In this study computer-assi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0865

    authors： Yan X,Hollis T,Svinth M,Day P,Monzingo AF,Milne GW,Robertus JD

    更新日期：1997-03-14 00:00:00

  • Long range base-pairing in the leftward transcription unit of bacteriophage lambda. Characterization by electron microscopy and computer-aided sequence analysis.

    abstract：:Restriction fragments of bacteriophage lambda DNA corresponding to the major leftward transcription unit were purified, denatured to form single-stranded DNA, self-annealed, and examined by electron microscopy. Three intrastrand stem and loop secondary structures were observed reproducibly and the locations of the pai...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90070-6

    authors： Edlind TD,Cooley TE,Richards SH,Ihler GM

    更新日期：1984-11-05 00:00:00

  • Novel Insights into Cell Entry of Emerging Human Pathogenic Arenaviruses.

    abstract：:Viral hemorrhagic fevers caused by emerging RNA viruses of the Arenavirus family are among the most devastating human diseases. Climate change, global trade, and increasing urbanization promote the emergence and re-emergence of these human pathogenic viruses. Emerging pathogenic arenaviruses are of zoonotic origin and...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2018.04.026

    authors： Fedeli C,Moreno H,Kunz S

    更新日期：2018-06-22 00:00:00

  • The structure and computational analysis of Mycobacterium tuberculosis protein CitE suggest a novel enzymatic function.

    abstract：:Fatty acid biosynthesis is essential for the survival of Mycobacterium tuberculosis and acetyl-coenzyme A (acetyl-CoA) is an essential precursor in this pathway. We have determined the 3-D crystal structure of M. tuberculosis citrate lyase beta-subunit (CitE), which as annotated should cleave protein bound citryl-CoA ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.09.086

    authors： Goulding CW,Bowers PM,Segelke B,Lekin T,Kim CY,Terwilliger TC,Eisenberg D

    更新日期：2007-01-12 00:00:00

  • Crystal structure of the motor domain of the human kinetochore protein CENP-E.

    abstract：:The human kinetochore is a highly complex macromolecular structure that connects chromosomes to spindle microtubules (MTs) in order to facilitate accurate chromosome segregation. Centromere-associated protein E (CENP-E), a member of the kinesin superfamily, is an essential component of the kinetochore, since it is req...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.05.053

    authors： Garcia-Saez I,Yen T,Wade RH,Kozielski F

    更新日期：2004-07-23 00:00:00

  • Human transcriptional coactivator PC4 stimulates DNA end joining and activates DSB repair activity.

    abstract：:Human transcriptional coactivator PC4 is a highly abundant nuclear protein that is involved in diverse cellular processes ranging from transcription to chromatin organization. Earlier, we have shown that PC4, a positive activator of p53, overexpresses upon genotoxic insult in a p53-dependent manner. In the present stu...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.11.008

    authors： Batta K,Yokokawa M,Takeyasu K,Kundu TK

    更新日期：2009-01-23 00:00:00

  • Crystal structure of the dog lipocalin allergen Can f 2: implications for cross-reactivity to the cat allergen Fel d 4.

    abstract：:The dog lipocalin allergen Can f 2 is an important cause of allergic sensitization in humans worldwide. Here, the first crystal structure of recombinant rCan f 2 at 1.45 A resolution displays a classical lipocalin fold with a conserved Gly-Xaa-Trp motif, in which Trp19 stabilizes the overall topology of the monomeric ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.05.043

    authors： Madhurantakam C,Nilsson OB,Uchtenhagen H,Konradsen J,Saarne T,Högbom E,Sandalova T,Grönlund H,Achour A

    更新日期：2010-08-06 00:00:00

  • Translocon-Associated Protein Complex (TRAP) is Crucial for Co-Translational Translocation of Pre-Proinsulin.

    abstract：:Many unanswered questions remain in understanding the biosynthesis of the peptide hormone insulin. Here we elucidate new aspects in the mechanism of co-translational translocation initiation of pre-proinsulin in the endoplasmic reticulum. We utilize a translational arrest peptide derived from the x-box-binding protein...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.10.028

    authors： Kriegler T,Kiburg G,Hessa T

    更新日期：2020-12-04 00:00:00

  • Structure and Function of Viral Deubiquitinating Enzymes.

    abstract：:Post-translational modification of cellular proteins by ubiquitin regulates numerous cellular processes, including innate and adaptive immune responses. Ubiquitin-mediated control over these processes can be reversed by cellular deubiquitinating enzymes (DUBs), which remove ubiquitin from cellular targets and depolyme...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2017.06.010

    authors： Bailey-Elkin BA,Knaap RCM,Kikkert M,Mark BL

    更新日期：2017-11-10 00:00:00

  • Sigma competition: the contest between bacteriophage T4 middle and late transcription.

    abstract：:In bacterial transcription, diverse sigma-family promoter recognition proteins compete for a common RNA polymerase core. Bacteriophage T4 infection ultimately reduces this competition to a duel between activated viral middle and enhanced late transcription, involving two sigma proteins, two phage-encoded activator pro...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.2953

    authors： Kolesky S,Ouhammouch M,Brody EN,Geiduschek EP

    更新日期：1999-08-13 00:00:00

  • Engineered Bacillus lentus subtilisins having altered flexibility.

    abstract：:The three-dimensional structures of engineered variants of Bacillus lentus subtilisin having increased enzymatic activity, K27R/N87S/V104Y/N123S/T274A (RSYSA) and N76D/N87S/S103A/V104I (DSAI), were determined by X-ray crystallography. In addition to identifying changes in atomic position we report a method that identi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3033

    authors： Graycar T,Knapp M,Ganshaw G,Dauberman J,Bott R

    更新日期：1999-09-10 00:00:00

  • Prediction of catalytic residues in enzymes based on known tertiary structure, stability profile, and sequence conservation.

    abstract：:The catalytic or functionally important residues of a protein are known to exist in evolutionarily constrained regions. However, the patterns of residue conservation alone are sometimes not very informative, depending on the homologous sequences available for a given query protein. Here, we present an integrated metho...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00207-9

    authors： Ota M,Kinoshita K,Nishikawa K

    更新日期：2003-04-11 00:00:00

  • Cycling of the Sm-like protein Hfq on the DsrA small regulatory RNA.

    abstract：:Small RNAs (sRNAs) regulate bacterial genes involved in environmental adaptation. This RNA regulation requires Hfq, a bacterial Sm-like protein that stabilizes sRNAs and enhances RNA-RNA interactions. To understand the mechanism of target recognition by sRNAs, we investigated the interactions between Hfq, the sRNA Dsr...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.10.006

    authors： Lease RA,Woodson SA

    更新日期：2004-12-10 00:00:00

  • In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities.

    abstract：:Human pseudouridine (Ψ) synthase Pus1 (hPus1) modifies specific uridine residues in several non-coding RNAs: tRNA, U2 spliceosomal RNA, and steroid receptor activator RNA. We report three structures of the catalytic core domain of hPus1 from two crystal forms, at 1.8Å resolution. The structures are the first of a mamm...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2013.05.014

    authors： Czudnochowski N,Wang AL,Finer-Moore J,Stroud RM

    更新日期：2013-10-23 00:00:00

  • Solution state NMR structure and dynamics of KpOmpA, a 210 residue transmembrane domain possessing a high potential for immunological applications.

    abstract：:The three-dimensional structure of the outer membrane protein A from Klebsiella pneumoniae transmembrane domain was determined by NMR.This protein induces specific humoral and cytotoxic responses, and is a potent carrier protein. This is one of the largest integral membrane proteins(210 residues) for which nearly comp...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.10.021

    authors： Renault M,Saurel O,Czaplicki J,Demange P,Gervais V,Löhr F,Réat V,Piotto M,Milon A

    更新日期：2009-01-09 00:00:00

  • Both binding sites of the starch-binding domain of Aspergillus niger glucoamylase are essential for inducing a conformational change in amylose.

    abstract：:The interaction of the two binding sites of the starch-binding domain (SBD) of Aspergillus niger glucoamylase 1 (GA-I) with substrate has been investigated by using atomic force microscopy (AFM) and UV difference spectroscopy in combination with site-specific mutants of both SBD and GA-I. The SBD possesses two binding...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5097

    authors： Giardina T,Gunning AP,Juge N,Faulds CB,Furniss CS,Svensson B,Morris VJ,Williamson G

    更新日期：2001-11-09 00:00:00