Abstract:
:Human transcriptional coactivator PC4 is a highly abundant nuclear protein that is involved in diverse cellular processes ranging from transcription to chromatin organization. Earlier, we have shown that PC4, a positive activator of p53, overexpresses upon genotoxic insult in a p53-dependent manner. In the present study, we show that PC4 stimulates ligase-mediated DNA end joining irrespective of the source of DNA ligase. Pull-down assays reveal that PC4 helps in the association of DNA ends through its C-terminal domain. In vitro nonhomologous end-joining assays with cell-free extracts show that PC4 enhances the joining of noncomplementary DNA ends. Interestingly, we found that PC4 activates double-strand break (DSB) repair activity through stimulation of DSB rejoining in vivo. Together, these findings demonstrate PC4 as an activator of nonhomologous end joining and DSB repair activity.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Batta K,Yokokawa M,Takeyasu K,Kundu TKdoi
10.1016/j.jmb.2008.11.008subject
Has Abstractpub_date
2009-01-23 00:00:00pages
788-99issue
3eissn
0022-2836issn
1089-8638pii
S0022-2836(08)01413-7journal_volume
385pub_type
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