Structure of nucleosomal DNA at high salt concentration as probed by hydroxyl radical.

abstract：:The effect of divalent cations on the self-association of high molecular weight subfragment-2 (long S-2) and low molecular weight subfragment-2 (short S-2) of rabbit skeletal muscle myosin has been investigated. In the presence of millimolar concentrations of Ca2+ or Mg2+ long S-2 associates at neutral pH to form orde...

journal_title：Journal of molecular biology

authors： Ueno H,Rodgers ME,Harrington WF

更新日期：1983-08-05 00:00:00

abstract：:A multiple alignment of five (beta/alpha)8-barrel enzymes has been derived from their structure. The eight beta-strands and eight alpha-helices of the (beta/alpha)8-barrel are correctly aligned and the equivalenced residues in these regions fulfil similar structural roles. Each beta-strand has a central core of usuall...

journal_title：Journal of molecular biology

authors： Pickett SD,Saqi MA,Sternberg MJ

更新日期：1992-11-05 00:00:00

abstract：:Large rigid-body domain movements are critical to GroEL-mediated protein folding, especially apical domain elevation and twist associated with the formation of a folding chamber upon binding ATP and co-chaperonin GroES. Here, we have modeled the anisotropic displacements of GroEL domains from various crystallized stat...

journal_title：Journal of molecular biology

authors： Chaudhry C,Horwich AL,Brunger AT,Adams PD

更新日期：2004-09-03 00:00:00

abstract：:The reverse transcriptase (RT) of HIV-1 and feline immunodeficiency virus (FIV) consist of two subunits of 51 kDa (p51) and 66 kDa (p66). In order to elucidate the role of p51 in the heterodimer, chimeric HIV-1/FIV RT heterodimers were constructed and characterized. The FIV RT p51/HIV-1 RT p66 chimera showed a 2.5-fol...

journal_title：Journal of molecular biology

authors： Amacker M,Hübscher U

更新日期：1998-05-15 00:00:00

abstract：:Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that displays several non-glycolytic activities, including the maintenance and/or protection of telomeres. In this study, we determined the molecular mechanism and biological role of the interaction between GAPDH and human telomeric DNA. Using gel...

journal_title：Journal of molecular biology

authors： Demarse NA,Ponnusamy S,Spicer EK,Apohan E,Baatz JE,Ogretmen B,Davies C

更新日期：2009-12-11 00:00:00

abstract：:A systematic analysis of peptide nucleic acid (PNA) complexes deposited in the Protein Data Bank has been carried out using a set of contiguous atom torsion angle definitions. The analysis is complemented by molecular mechanics adiabatic potential energy calculations on hybrid PNA-nucleic acid model systems. Hitherto ...

journal_title：Journal of molecular biology

authors： Topham CM,Smith JC

更新日期：1999-10-08 00:00:00

abstract：:The essential cell division genes ftsQ, ftsA and ftsZ map in a cluster of cell envelope genes located at two minutes on the Escherichia coli genetic map and appear to constitute an atypical operon. These closely clustered genes are all transcribed in the same direction and yet each of these genes was independently clo...

journal_title：Journal of molecular biology

authors： Yi QM,Rockenbach S,Ward JE Jr,Lutkenhaus J

更新日期：1985-08-05 00:00:00

abstract：:During general genetic recombination and recombinational DNA repair, DNA damages and heterologies are often encountered which must be efficiently processed by the cellular recombination machinery. In RecA-mediated three-strand exchange reactions between single-stranded circular and linear duplex DNA, or four-strand ex...

journal_title：Journal of molecular biology

authors： Adams DE,West SC

更新日期：1996-11-08 00:00:00

abstract：:Natriuretic peptides are cyclic vasoactive peptide hormones with great diagnostic and therapeutic relevance. The main catabolic pathway postulated for natriuretic peptides is the degradation by neutral endopeptidase (NEP). However, B-type natriuretic peptide has been found to be resistant to NEP. Here, we compared the...

journal_title：Journal of molecular biology

authors： Pankow K,Schwiebs A,Becker M,Siems WE,Krause G,Walther T

更新日期：2009-10-23 00:00:00

abstract：:The Runt domain is the DNA binding domain of the core binding factor (CBF) Runx subunits. The CBFs are transcription factors that play critical roles in hematopoiesis, bone, and neuron development in mammals. A common non-DNA binding CBFbeta subunit heterodimerizes with the Runt domain of the Runx proteins and alloste...

journal_title：Journal of molecular biology

authors： Li Z,Lukasik SM,Liu Y,Grembecka J,Bielnicka I,Bushweller JH,Speck NA

更新日期：2006-12-15 00:00:00

abstract：:The Saccharomyces cerevisiae non-Mendelian factor [URE3] propagates by a prion-like mechanism, involving aggregation of the chromosomally encoded protein Ure2. The N-terminal prion domain (PrD) of Ure2 is required for prion activity in vivo and amyloid formation in vitro. However, the molecular mechanism of the prion-...

journal_title：Journal of molecular biology

authors： Galani D,Fersht AR,Perrett S

更新日期：2002-01-11 00:00:00

abstract：:The folding/unfolding pathway of barnase has been studied extensively using the protein engineering method, which has provided indirect structural information for the transition state and the major folding intermediate. To further characterize the structural properties of the intermediate, we have simulated the therma...

journal_title：Journal of molecular biology

authors： Li A,Daggett V

更新日期：1998-01-30 00:00:00

abstract：:The secondary structure of porin, maltoporin and OmpA protein reconstituted in lipid membranes is determined by Raman spectroscopy. The three proteins have similar structures consisting of 50 to 60% beta-strand, about 20% beta-turn, and less than 15% alpha-helix. Employing a method for structural prediction that accou...

journal_title：Journal of molecular biology

authors： Vogel H,Jähnig F

更新日期：1986-07-20 00:00:00

abstract：:alpha-Lactalbumin (alpha-LA) is a two-domain, calcium-binding protein that forms one of the best studied molten globules. We present here amide hydrogen exchange studies of the molten globule formed by human alpha-LA at pH 2 and compare these results with a similar study of the native state at pH 6.3. The most persist...

journal_title：Journal of molecular biology

authors： Schulman BA,Redfield C,Peng ZY,Dobson CM,Kim PS

更新日期：1995-11-10 00:00:00

abstract：:Pathogenic Vibrio cholerae secrete V. cholerae cytolysin (VCC), an 80 kDa pro-toxin that assembles into an oligomeric pore on target cell membranes following proteolytic cleavage and interaction with cell surface receptors. To gain insight into the activation and targeting activities of VCC, we solved the crystal stru...

journal_title：Journal of molecular biology

authors： Olson R,Gouaux E

更新日期：2005-07-29 00:00:00

abstract：:In recent years, increased interest has been directed towards hydrogen sulfide (H2S) as the third gasotransmitter and its role in various diseases. Cystathionine-gamma-lyase (CSE) is one of the enzymes responsible for the endogenous production of H2S in mammals. With the aid of the crystal structures of human CSE and ...

journal_title：Journal of molecular biology

authors： Huang S,Chua JH,Yew WS,Sivaraman J,Moore PK,Tan CH,Deng LW

更新日期：2010-02-26 00:00:00

abstract：:α-Synuclein (αSyn) is an intrinsically disordered protein that can form amyloid fibrils. Fibrils of αSyn are implicated with the pathogenesis of Parkinson's disease and other synucleinopathies. Elucidating the mechanism of fibril formation of αSyn is therefore important for understanding the mechanism of the pathogene...

journal_title：Journal of molecular biology

authors： Fujiwara S,Kono F,Matsuo T,Sugimoto Y,Matsumoto T,Narita A,Shibata K

更新日期：2019-08-09 00:00:00

abstract：:Calmodulin (CaM) is an important regulator of Kv7.x (KCNQx) voltage-gated potassium channels. Channels from this family produce neuronal M currents and cardiac and auditory I(KS) currents and harbor mutations that cause arrhythmias, epilepsy, and deafness. Despite extensive functional characterization, biochemical and...

journal_title：Journal of molecular biology

authors： Xu Q,Chang A,Tolia A,Minor DL Jr

更新日期：2013-01-23 00:00:00

abstract：:The crystal structure of cyanovirin-N (CV-N), a protein with potent antiviral activity, was solved at 1.5 A resolution by molecular replacement using as the search model the solution structure previously determined by NMR. The crystals belong to the space group P3221 with one monomer of CV-N in each asymmetric unit. T...

journal_title：Journal of molecular biology

authors： Yang F,Bewley CA,Louis JM,Gustafson KR,Boyd MR,Gronenborn AM,Clore GM,Wlodawer A

更新日期：1999-05-07 00:00:00

abstract：:Histone deacetylases (HDACs) modify core histones and participate in large regulatory complexes that both suppress and enhance transcription. Recent studies indicate that some HDACs can act on non-histone proteins as well. Interest in these enzymes is growing because HDAC inhibitors appear to be promising therapeutic ...

journal_title：Journal of molecular biology

authors： Gregoretti IV,Lee YM,Goodson HV

更新日期：2004-04-16 00:00:00

abstract：:Loss-of-function mutations in the gene encoding the multifunctional protein, DJ-1, have been implicated in the pathogenesis of early-onset familial Parkinson's disease (PD), suggesting that DJ-1 may act as a neuroprotectant for dopaminergic (DA) neurons. Enhanced autophagy may benefit PD by clearing damaged organelles...

journal_title：Journal of molecular biology

authors： Gao H,Yang W,Qi Z,Lu L,Duan C,Zhao C,Yang H

更新日期：2012-10-19 00:00:00

abstract：:The isolated H(+)-ATPase from Escherichia coli (EF(0)F(1)) was investigated by electron microscopy of samples of negatively stained monodisperse molecules, followed by single-particle image processing. The resulting three-dimensional maps showed that the F(1)-part is connected by a prominent stalk to a more peripheral...

journal_title：Journal of molecular biology

authors： Böttcher B,Bertsche I,Reuter R,Gräber P

更新日期：2000-02-18 00:00:00

abstract：:Though up to 20% of the total RNA in bacterial cells is tRNA, the regulation of tRNA distribution on the genomic level remains unclear. tRNA distribution is governed by four processes: transcription, processing of precursor tRNA, degradation of precursor tRNA and degradation of mature tRNA. To elucidate the relationsh...

journal_title：Journal of molecular biology

authors： Dittmar KA,Mobley EM,Radek AJ,Pan T

更新日期：2004-03-12 00:00:00

abstract：:The recA protein from Proteus mirabilis, which is homologous to the Escherichia coli protein, forms crystals in the orthorhombic space group P2(1)2(1)2(1). There are two 38,000 molecular weight subunits in the asymmetric unit and the unit cell dimensions are a = 57.5, b = 127.0 and c = 157.0 A. ...

journal_title：Journal of molecular biology

authors： Weber IT,Steitz TA

更新日期：1986-03-05 00:00:00

abstract：:I present an electron microscopical analysis of the columnar hexagonal liquid crystalline phase of DNA. Freeze-fracture methods reveal that this phase is a lamellar structure, each layer (30 to 40 A thick) composed of DNA molecules aligned in parallel. Numerous defects can be seen in the structure, and their nature is...

journal_title：Journal of molecular biology

authors： Livolant F

更新日期：1991-03-05 00:00:00

abstract：:The betagamma-crystallin superfamily consists of a class of homologous two-domain proteins with Greek-key fold. Protein S, a Ca(2+)-binding spore-coat protein from the soil bacterium Myxococcus xanthus exhibits a high degree of sequential and structural homology with gammaB-crystallin from the vertebrate eye lens. In ...

journal_title：Journal of molecular biology

authors： Wenk M,Jaenicke R

更新日期：1999-10-15 00:00:00

abstract：:An accurate three-dimensional structure is known for papain (1.65 A resolution) and actinidin (1.7 A). A detailed comparison of these two structures was performed to determine the effect of amino acid changes on the conformation. It appeared that, despite only 48% identity in their amino acid sequence, different cryst...

journal_title：Journal of molecular biology

authors： Kamphuis IG,Drenth J,Baker EN

更新日期：1985-03-20 00:00:00

abstract：:Tau displacement from microtubules is the first step in the onset of tauopathies and is followed by toxic protein aggregation. However, other non-canonical functions of Tau might have a role in these pathologies. Here, we demonstrate that a small amount of Tau localizes in the nuclear compartment and accumulates in bo...

journal_title：Journal of molecular biology

authors： Siano G,Varisco M,Caiazza MC,Quercioli V,Mainardi M,Ippolito C,Cattaneo A,Di Primio C

更新日期：2019-02-15 00:00:00

abstract：:The Janus kinases (JAKs) are a pivotal family of protein tyrosine kinases (PTKs) that play prominent roles in numerous cytokine signaling pathways, with aberrant JAK activity associated with a variety of hematopoietic malignancies, cardiovascular diseases and immune-related disorders. Whereas the structures of the JAK...

journal_title：Journal of molecular biology

authors： Williams NK,Bamert RS,Patel O,Wang C,Walden PM,Wilks AF,Fantino E,Rossjohn J,Lucet IS

更新日期：2009-03-20 00:00:00

abstract：:Cytosine deaminase (CD) catalyzes the deamination of cytosine, producing uracil. This enzyme is present in prokaryotes and fungi (but not multicellular eukaryotes) and is an important member of the pyrimidine salvage pathway in those organisms. The same enzyme also catalyzes the conversion of 5-fluorocytosine to 5-flu...

journal_title：Journal of molecular biology

authors： Ireton GC,McDermott G,Black ME,Stoddard BL

更新日期：2002-01-25 00:00:00