Different subdomains are most protected from hydrogen exchange in the molten globule and native states of human alpha-lactalbumin.

abstract：:Low molecular weight protein tyrosine phosphatase (LMW-PTP) dimerizes in the phosphate-bound state in solution with a dissociation constant of K(d)=1.5(+/-0.1)mM and an off-rate on the order of 10(4)s(-1). 1H and 15N NMR chemical shifts identify the dimer interface, which is in excellent agreement with that observed i...

journal_title：Journal of molecular biology

authors： Akerud T,Thulin E,Van Etten RL,Akke M

更新日期：2002-09-06 00:00:00

abstract：:The X-ray crystal structures of Thermoactinomyces vulgaris R-47 alpha-amylase 1 (TVAI) and alpha-amylase 2 (TVAII) have been determined at 1.6 A and 2.3 A resolution, respectively. The structures of TVAI and TVAII have been refined, R-factor of 0.182 (R(free)=0.206) and 0.179 (0.224), respectively, with good chemical ...

journal_title：Journal of molecular biology

authors： Kamitori S,Abe A,Ohtaki A,Kaji A,Tonozuka T,Sakano Y

更新日期：2002-04-26 00:00:00

abstract：:Polyamines are abundant metabolites that directly influence gene expression. Although the role of polyamines in DNA condensation is well known, their role in RNA folding is less understood. Non-denaturing gel electrophoresis was used to monitor the equilibrium folding transitions of the Tetrahymena ribozyme in the pre...

journal_title：Journal of molecular biology

authors： Koculi E,Lee NK,Thirumalai D,Woodson SA

更新日期：2004-07-30 00:00:00

abstract：:The work reported here describes insertion-deletion (Indel) polymorphisms in two internally eliminated sequences (IESs, that are deleted during development in Tetrahymena): a 1.8 kb Indel at one end of the 1.1 kb H1 IES and a 0.5 kb Indel inside the 1.4 kb calmodulin (C) IES. These two IESs are located in the proximit...

journal_title：Journal of molecular biology

authors： Huvos P

更新日期：2004-03-05 00:00:00

abstract：:In-vivo studies have demonstrated that adenovirus type 2 and adenovirus type 4 have different DNA sequence requirements for the initiation of DNA replication. To investigate the basis of these differences an in-vitro system has been developed which will faithfully initiate adenovirus type 4 DNA replication. A plasmid ...

journal_title：Journal of molecular biology

authors： Harris MP,Hay RT

更新日期：1988-05-05 00:00:00

abstract：:The 90-kDa heat shock protein (Hsp90) is a widely conserved and ubiquitous molecular chaperone that participates in ATP-dependent protein remodeling in both eukaryotes and prokaryotes. It functions in conjunction with Hsp70 and the Hsp70 cochaperones, an Hsp40 (J-protein) and a nucleotide exchange factor. In Escherich...

journal_title：Journal of molecular biology

authors： Kravats AN,Doyle SM,Hoskins JR,Genest O,Doody E,Wickner S

更新日期：2017-03-24 00:00:00

abstract：:Kinesin superfamily molecular motors step along microtubules (MTs) via a cycle of conformational changes which is coupled to ATP turnover. To probe the coupling mechanism, we titrated the effects of various nucleotides on MT binding by two superfamily members; MT plus-end-directed kinesin and MT minus-end-directed non...

journal_title：Journal of molecular biology

authors： Crevel IM,Lockhart A,Cross RA

更新日期：1996-03-22 00:00:00

abstract：:Bulges represent one of the most common non-helical elements in RNA, often displaying a strong degree of phylogenetic conservation, both in location and sequence, within larger RNA molecules. Thus, knowledge of the conformation and flexibility of RNA bulges is an important prerequisite for understanding the rules gove...

journal_title：Journal of molecular biology

authors： Zacharias M,Hagerman PJ

更新日期：1995-03-31 00:00:00

abstract：:The three-dimensional structure of a site-directed mutant of the bacterially expressed Fv fragment from monoclonal antibody D1.3, complexed to the specific antigen lysozyme has been determined to a nominal resolution of 1.8 A using X-ray diffraction data. The replacement of VL Trp92 by Asp allows two water molecules t...

journal_title：Journal of molecular biology

authors： Ysern X,Fields BA,Bhat TN,Goldbaum FA,Dall'Acqua W,Schwarz FP,Poljak RJ,Mariuzza RA

更新日期：1994-05-13 00:00:00

abstract：:Assembly of infectious retroviral particles involves recognition of specific sequences on the viral RNA by the nucleocapsid (NC) domain of the Gag polyprotein, and subsequent stoichiometric binding of the processed NC protein along the entire length of the RNA. NC proteins also act as nucleic acid chaperones. They acc...

journal_title：Journal of molecular biology

authors： Urbaneja MA,Wu M,Casas-Finet JR,Karpel RL

更新日期：2002-05-03 00:00:00

abstract：:TF1 is a member of the family of type II DNA-binding proteins, which also includes the bacterial HU proteins and the Escherichia coli integration host factor (IHF). Distinctive to TF1, which is encoded by the Bacillus subtilis bacteriophage SPO1, is its preferential binding to DNA in which thymine is replaced by 5-hyd...

journal_title：Journal of molecular biology

authors： Grove A,Galeone A,Mayol L,Geiduschek EP

更新日期：1996-07-12 00:00:00

abstract：:Transcription of the repA gene of the Pseudomonas plasmid pPS10 is initiated from a sigma 70 type promoter located 81 bp upstream from the repA gene, extends through the repA gene and the adjacent open reading frame, and ends 1114 nucleotides downstream. The repA promoter is repressed by interactions of the RepA prote...

journal_title：Journal of molecular biology

authors： García de Viedma D,Giraldo R,Ruiz-Echevarría MJ,Lurz R,Díaz-Orejas R

更新日期：1995-03-24 00:00:00

abstract：:Small RNAs (sRNAs) regulate bacterial genes involved in environmental adaptation. This RNA regulation requires Hfq, a bacterial Sm-like protein that stabilizes sRNAs and enhances RNA-RNA interactions. To understand the mechanism of target recognition by sRNAs, we investigated the interactions between Hfq, the sRNA Dsr...

journal_title：Journal of molecular biology

authors： Lease RA,Woodson SA

更新日期：2004-12-10 00:00:00

abstract：:The kink turn (K-turn) is a common motif in RNA structure, found in many RNA species important in translation, RNA modification and splicing, and the control of gene expression. In general the K-turn comprises a three nucleotide bulge followed by trans sugar-Hoogsteen G.A pairs. The RNA adopts a tightly kinked conform...

journal_title：Journal of molecular biology

authors： Turner B,Lilley DM

更新日期：2008-08-29 00:00:00

abstract：:Tafazzin is a mitochondrial enzyme that exchanges fatty acids between phospholipids by phospholipid-lysophospholipid transacylation. The reaction alters the molecular species composition and, as a result, the physical properties of lipids. In vivo, the most important substrate of tafazzin is the mitochondria-specific ...

journal_title：Journal of molecular biology

authors： Schlame M,Xu Y

更新日期：2020-08-21 00:00:00

abstract：:Theoretical methods for correlation of sequence changes and redox potential of electron transport proteins are examined using the Asn52----Ile mutation in cytochrome c as a test case. The first approach uses the protein dipoles Langevin dipoles (PDLD) method and the high resolution X-ray structures of the native and t...

journal_title：Journal of molecular biology

authors： Langen R,Brayer GD,Berghuis AM,McLendon G,Sherman F,Warshel A

更新日期：1992-04-05 00:00:00

abstract：:Kinetic and thermodynamic parameters, derived from 1H-NMR measurements of the imino proton exchange rates upon titration with the exchange catalyst ammonia, are reported for two mixed-sequence peptide nucleic acid (PNA)-DNA hybrids and their counterpart DNA duplex. The exchange times of the imino protons in the PNA st...

journal_title：Journal of molecular biology

authors： Leijon M,Sehlstedt U,Nielsen PE,Gräslund A

更新日期：1997-08-22 00:00:00

abstract：:Streptococcus mutans is the primary etiological agent of dental caries in man and other mammalian organisms. This bacterium metabolizes carbohydrates actively and thrives under anaerobic conditions by fermenting l-ascorbate (Asc) via the sga operon, which includes SgaT, PtxB, and PtxA. These three proteins are members...

journal_title：Journal of molecular biology

authors： Lei J,Li LF,Su XD

更新日期：2009-02-20 00:00:00

abstract：:SHP, the capsid-stabilizing protein of lambdoid phage 21, is highly resistant against denaturant-induced unfolding. We demonstrate that this high functional stability of SHP is due to a high kinetic stability with a half-life for unfolding of 25 days at zero denaturant, while the thermodynamic stability is not unusual...

journal_title：Journal of molecular biology

authors： Forrer P,Chang C,Ott D,Wlodawer A,Plückthun A

更新日期：2004-11-12 00:00:00

abstract：:Herpes simplex viruses (HSVs) are human pathogens that establish lytic and latent infections. Reactivation from latency occurs intermittently, which represents a lifelong source of recurrent infection. In this complex process, HSV triggers and neutralizes innate immunity. Therefore, a dynamic equilibrium between HSV a...

journal_title：Journal of molecular biology

authors： Ma Y,He B

更新日期：2014-03-20 00:00:00

abstract：:We have developed a procedure in which disulfide cross-links are used to identify regions of proteins that undergo functionally important intramolecular motion. The approach was applied to the identification of disulfide bonds that stabilize the active state of the yeast α-mating pheromone receptor Ste2p, a member of ...

journal_title：Journal of molecular biology

authors： Taslimi A,Mathew E,Celić A,Wessel S,Dumont ME

更新日期：2012-05-18 00:00:00

abstract：:The FLP recombinase is encoded by the yeast plasmid 2 microns circle and catalyses a site-specific recombination reaction that results in inversion of a segment of the 2 micron plasmid. We describe a method for the isolation of inactivating mutations in the FLP gene. The analysis of the recombination and binding activ...

journal_title：Journal of molecular biology

authors： Schwartz CJ,Sadowski PD

更新日期：1989-02-20 00:00:00

abstract：:Harold Erickson has recently provided a useful analysis of helical structures having one class versus two classes of intersubunit bonds. His analysis is based upon an assumption that the subunits themselves are essentially unchanged upon bond formation (polymerization). He shows that such a structure having two classe...

journal_title：Journal of molecular biology

authors： Egelman EH,DeRosier DJ

更新日期：1991-02-05 00:00:00

abstract：:A long-standing problem of molecular biology is the prediction of globular protein tertiary structure from the primary sequence. In the context of a new, 24-nearest-neighbor lattice model of proteins that includes both alpha and beta-carbon atoms, the requirements for folding to a unique four-member beta-barrel, four-...

journal_title：Journal of molecular biology

authors： Skolnick J,Kolinski A

更新日期：1991-09-20 00:00:00

abstract：:Protein allostery plays a pivotal role in many regulatory processes. Prominent examples are cell-surface receptors, which allosterically transmit ligand-generated signals to their cytoplasmic domains, or bacterial transcription factors, which alternate between a free conformation and a DNA-bound conformation in respon...

journal_title：Journal of molecular biology

authors： Goeke D,Kaspar D,Stoeckle C,Grubmüller S,Berens C,Klotzsche M,Hillen W

更新日期：2012-02-10 00:00:00

abstract：:Reverse transcriptase, an essential retroviral DNA polymerase, replicates the single-stranded RNA genome of the retrovirus, producing a double-stranded DNA copy, which is subsequently integrated into the host's genome. Substitution of Ala for either Asp114 or Arg116, two highly conserved residues in the fingers domain...

journal_title：Journal of molecular biology

authors： Gu J,Villanueva RA,Snyder CS,Roth MJ,Georgiadis MM

更新日期：2001-01-12 00:00:00

abstract：:The motion of the atoms in the small protein bovine pancreatic trypsin inhibitor has been simulated for about 60 picoseconds using two different potential energy functions. In one known as HHL the hydrogen bond is purely electrostatic, in the other, known as L79, the hydrogen bond is a directional O . . . H interactio...

journal_title：Journal of molecular biology

authors： Levitt M

更新日期：1983-08-15 00:00:00

abstract：:The human Y RNAs, small RNAs with an unknown function, are complexed with at least three proteins: the 60,000 M(r) Ro protein (Ro60), the 52,000 M(r) Ro protein (Ro52) and the La protein (La). In this study we examined the intermolecular interactions between the components of these so-called Ro ribonucleoprotein (Ro R...

journal_title：Journal of molecular biology

authors： Slobbe RL,Pluk W,van Venrooij WJ,Pruijn GJ

更新日期：1992-09-20 00:00:00

abstract：:We report the results of an NMR study of unlabeled and uniformly (13)C,(15)N-labeled d(G(3)AG(2)T(3)G(3)AT) in 100 mM NaCl, conditions under which it forms a dimeric quadruplex containing several new topological features. The DNA oligomer chain in each symmetry-related monomer subunit undergoes three sharp turns to fo...

journal_title：Journal of molecular biology

authors： Zhang N,Gorin A,Majumdar A,Kettani A,Chernichenko N,Skripkin E,Patel DJ

更新日期：2001-08-31 00:00:00

abstract：:The development of drug resistance has long plagued our efforts to curtail viral infections in general and influenza in particular. The problem is particularly challenging since the exact mode of resistance may be difficult to predict, without waiting for untreatable strains to evolve. Herein, a different approach is ...

journal_title：Journal of molecular biology

authors： Assa D,Alhadeff R,Krugliak M,Arkin IT

更新日期：2016-10-09 00:00:00