Crystallization of recA protein from Proteus mirabilis.

abstract：:The methylation-dependent restriction endonuclease McrBC from Escherichia coli K12 cleaves DNA containing two R(m)C dinucleotides separated by about 40 to 2000 base-pairs. McrBC is unique in that cleavage is totally dependent on GTP hydrolysis. McrB is the GTP binding and hydrolyzing subunit, whereas MrC stimulates it...

journal_title：Journal of molecular biology

authors： Pieper U,Schweitzer T,Groll DH,Gast FU,Pingoud A

更新日期：1999-09-24 00:00:00

abstract：:Neurodegenerative disorders associated with protein misfolding are fatal diseases that are caused by fibrillation of endogenous proteins such as α-synuclein (α-syn) in Parkinson's disease (PD) or amyloid-β in Alzheimer's disease. Fibrils of α-syn are a major pathological hallmark of PD and certain aggregation intermed...

journal_title：Journal of molecular biology

authors： Wördehoff MM,Bannach O,Shaykhalishahi H,Kulawik A,Schiefer S,Willbold D,Hoyer W,Birkmann E

更新日期：2015-03-27 00:00:00

abstract：:Alphavirus replicons are very useful for analyzing different aspects of viral molecular biology. They are also useful tools in the development of new vaccines and highly efficient expression of heterologous genes. We have investigated the translatability of Sindbis virus (SV) subgenomic mRNA bearing different 5'-untra...

journal_title：Journal of molecular biology

authors： Sanz MA,Welnowska E,Redondo N,Carrasco L

更新日期：2010-09-10 00:00:00

abstract：:The periplasmic C-terminal domain of the Escherichia coli DsbD protein (cDsbD) has a thioredoxin fold. The two cysteine residues in the CXXC motif serve as the reductant for the disulfide bond of the N-terminal domain which can in turn act as a reductant for various periplasmic partners. The resulting disulfide bond i...

journal_title：Journal of molecular biology

authors： Mavridou DA,Stevens JM,Ferguson SJ,Redfield C

更新日期：2007-07-20 00:00:00

abstract：:Phenol-soluble modulin α3 (PSMα3) is a functional amyloid secreted by the pathogenic bacterium Staphylococcus aureus. This 22-residue peptide serves as a key virulence determinant, toxic to human cells via the formation of unique cross-α amyloid-like fibrils. We demonstrate that bilayer vesicles accelerated PSMα3 fibr...

journal_title：Journal of molecular biology

authors： Malishev R,Tayeb-Fligelman E,David S,Meijler MM,Landau M,Jelinek R

更新日期：2018-05-11 00:00:00

abstract：:The X-ray crystal structure of a complex of a modified recombinant kringle-2 domain of human plasminogen, K2Pg[C4G/E56D/L72Y] (mK2Pg), containing an upregulated lysine-binding site, bound to a functional 30 residue internal peptide (VEK-30) from an M-type protein of a group A Streptococcus surface protein, has been de...

journal_title：Journal of molecular biology

authors： Rios-Steiner JL,Schenone M,Mochalkin I,Tulinsky A,Castellino FJ

更新日期：2001-05-11 00:00:00

abstract：:DNA structures bound to transcription factors are studied by using the crystal coordinates of complexes. Structural characteristics which are found at the sites where a protein secondary structure, an alpha-helix or a beta-sheet binds, can be understood in terms of fitting of the concave surface of the DNA major groov...

journal_title：Journal of molecular biology

authors： Suzuki M,Yagi N

更新日期：1996-02-09 00:00:00

abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

journal_title：Journal of molecular biology

authors： Wei Z,Xue Y,Xu H,Gong W

更新日期：2006-05-26 00:00:00

abstract：:A recently developed approach to calculate the pH dependence of protein stability from three-dimensional structure information is applied to the analysis of acid denaturation of sperm whale apomyoglobin. The finite difference Poisson-Boltzmann method is used to calculate pKa values and these are used to obtain titrati...

journal_title：Journal of molecular biology

authors： Yang AS,Honig B

更新日期：1994-04-15 00:00:00

abstract：:Ricin is a potent plant toxin which acts by removing a specific adenine residue from the ribosome. The X-ray crystal structure of a new, tetragonal crystal form of the recombinant ricin A-chain diffracting to 1.8 A resolution has been determined via molecular replacement methods and refined to a crystallographic R-fac...

journal_title：Journal of molecular biology

authors： Weston SA,Tucker AD,Thatcher DR,Derbyshire DJ,Pauptit RA

更新日期：1994-12-09 00:00:00

abstract：:We report the X-ray structure analysis and refinement at 1.9 A resolution of calf gamma-II crystallin, a lens-specific protein. The sequence of Croft (1972) has been modified to give a polypeptide chain of 174 residues (cf. 165). The protein has a symmetrical, hierarchical structure of two globular domains each compri...

journal_title：Journal of molecular biology

authors： Wistow G,Turnell B,Summers L,Slingsby C,Moss D,Miller L,Lindley P,Blundell T

更新日期：1983-10-15 00:00:00

abstract：:Siderophores are small-molecule iron chelators that many bacteria synthesize and secrete in order to survive in iron-depleted environments. Biosynthesis of enterobactin, the Escherichia coli catecholate siderophore, requires adenylation of 2,3-dihydroxybenzoic acid (2,3-DHB) by the cytoplasmic enzyme EntE. The DHB-AMP...

journal_title：Journal of molecular biology

authors： Khalil S,Pawelek PD

更新日期：2009-10-30 00:00:00

abstract：:Research on filoviruses has historically focused on the highly pathogenic ebola- and marburgviruses. Indeed, until recently, these were the only two genera in the filovirus family. Recent advances in sequencing technologies have facilitated the discovery of not only a new ebolavirus, but also three new filovirus gener...

journal_title：Journal of molecular biology

authors： Hume AJ,Mühlberger E

更新日期：2019-10-04 00:00:00

abstract：:A general mechanism for polymerase translocation is elaborated. The central feature of this mechanism is that a rapid translocational equilibrium is established after each cycle of nucleoside monophosphate incorporation such that the polymerase distributes itself by diffusional sliding between all accessible positions...

journal_title：Journal of molecular biology

authors： Guajardo R,Sousa R

更新日期：1997-01-10 00:00:00

abstract：:Synthesis of asparaginyl-tRNA (Asn-tRNA(Asn)) in bacteria can be formed either by directly ligating Asn to tRNA(Asn) using an asparaginyl-tRNA synthetase (AsnRS) or by synthesizing Asn on the tRNA. In the latter two-step indirect pathway, a non-discriminating aspartyl-tRNA synthetase (ND-AspRS) attaches Asp to tRNA(As...

journal_title：Journal of molecular biology

authors： Nair N,Raff H,Islam MT,Feen M,Garofalo DM,Sheppard K

更新日期：2016-02-13 00:00:00

abstract：:The 3.0-3.1A X-ray structures of the cytochrome b(6)f complex from Mastigocladus laminosus and Chlamydomonas reinhardtii obtained in the presence of the p-side quinone-analogue inhibitor tridecyl-stigmatellin (TDS) are very similar. A difference occurs in the p-side binding position of TDS. In C.reinhardtii, TDS binds...

journal_title：Journal of molecular biology

authors： Yan J,Cramer WA

更新日期：2004-11-19 00:00:00

abstract：:MazG is a homodimeric alpha-helical protein that belongs to the superfamily of all-alpha NTP pyrophosphatases. Its function has been connected to the regulation of the toxin-antitoxin module mazEF, implicated in programmed growth arrest/cell death of Escherichia coli cells under conditions of amino acid starvation. Th...

journal_title：Journal of molecular biology

authors： Drobnak I,Korencic A,Loris R,Marianovsky I,Glaser G,Jamnik A,Vesnaver G,Lah J

更新日期：2009-09-11 00:00:00

abstract：:Bifunctional human PAPS synthetase (PAPSS) catalyzes, in a two-step process, the formation of the activated sulfate carrier 3'-phosphoadenosine 5'-phosphosulfate (PAPS). The first reaction involves the formation of the 5'-adenosine phosphosulfate (APS) intermediate from ATP and inorganic sulfate. APS is then further p...

journal_title：Journal of molecular biology

authors： Sekulic N,Dietrich K,Paarmann I,Ort S,Konrad M,Lavie A

更新日期：2007-03-23 00:00:00

abstract：:Mutations in CAPN3/Capn3, which codes for skeletal muscle-specific calpain-3/p94 protease, are responsible for limb-girdle muscular dystrophy type 2A. Using "knock-in" (referred to as Capn3(CS/CS)) mice, in which the endogenous calpain-3 is replaced with a mutant calpain-3:C129S, which is a proteolytically inactive bu...

journal_title：Journal of molecular biology

authors： Ojima K,Ono Y,Ottenheijm C,Hata S,Suzuki H,Granzier H,Sorimachi H

更新日期：2011-04-01 00:00:00

abstract：:The functional properties of fetal bovine hemoglobin have been studied as a function of temperature, chloride and 2,3-diphosphoglycerate (DPG) concentration. The fetal bovine erythrocyte has six times the concentration of the allosteric modulator DPG compared with the adult cell, and yet the oxygen affinity of the fet...

journal_title：Journal of molecular biology

authors： Clementi ME,Scatena R,Mordente A,Condò SG,Castagnola M,Giardina B

更新日期：1996-01-12 00:00:00

abstract：:Riboswitches are noncoding RNA elements that are commonly found in the 5'-untranslated region of bacterial mRNA. Binding of a small-molecule metabolite to the riboswitch aptamer domain guides the folding of the downstream sequence into one of two mutually exclusive secondary structures that directs gene expression. Th...

journal_title：Journal of molecular biology

authors： Edwards AL,Batey RT

更新日期：2009-01-23 00:00:00

abstract：:The majority of the Mycobacterium tuberculosis response to hypoxia and nitric oxide is through the DosRS (DevRS) two-component regulatory system. The N-terminal input domain of the DosS sensor contains two GAF domains. We demonstrate here that the proximal GAF domain binds haem, and identified histidine 149 of DosS as...

journal_title：Journal of molecular biology

authors： Sardiwal S,Kendall SL,Movahedzadeh F,Rison SC,Stoker NG,Djordjevic S

更新日期：2005-11-11 00:00:00

abstract：:The majority of archaeal viral genes are of unknown function hindering our understanding of the virus life cycle and viral interactions with their host. Here, we first describe functional characterization of ORF131b (gp17) and ORF436 (gp18) of Sulfolobus islandicus rod-shaped virus 2 (SIRV2), both encoding proteins of...

journal_title：Journal of molecular biology

authors： Guo Y,Kragelund BB,White MF,Peng X

更新日期：2015-06-19 00:00:00

abstract：:beta-Crystallins are oligomeric eye lens proteins that are related to monomeric gamma-crystallins. The main sequence difference between the two families is the presence of sequence extensions in the beta-crystallins. A major question concerns the role that these extensions play in mediating interactions at the high pr...

journal_title：Journal of molecular biology

authors： Nalini V,Bax B,Driessen H,Moss DS,Lindley PF,Slingsby C

更新日期：1994-03-04 00:00:00

abstract：:The tetracycline analog 9-(N, N-dimethylglycylamido)-6-demethyl-6-deoxy-tetracycline (9glyTc) belongs to a new group of tetracyclines called glycylcyclines. They are strong antibiotics showing reduced sensitivity against the major tetracycline resistance mechanisms. We have determined the crystal structure of 9glyTc i...

journal_title：Journal of molecular biology

authors： Orth P,Schnappinger D,Sum PE,Ellestad GA,Hillen W,Saenger W,Hinrichs W

更新日期：1999-01-15 00:00:00

abstract：:The loop between the F and G beta strands (FG loop) of the bacteriophage MS2 coat protein subunit forms inter-subunit contacts around the 5-fold and 3-fold (quasi 6-fold) axes of the T=3 protein shell. In capsids, the loop is found in two very different conformations, one in B subunits, which form the 5-fold contact, ...

journal_title：Journal of molecular biology

authors： Stonehouse NJ,Valegård K,Golmohammadi R,van den Worm S,Walton C,Stockley PG,Liljas L

更新日期：1996-02-23 00:00:00

abstract：:Phosphorylation of short linear peptide motifs is a widespread process for the dynamic regulation of protein-protein interactions. However, the global impact of phosphorylation events on the protein-protein interactome is rarely addressed. The disordered C-terminal tail of ribosomal S6 kinase 1 (RSK1) binds to PDZ dom...

journal_title：Journal of molecular biology

authors： Gógl G,Biri-Kovács B,Durbesson F,Jane P,Nomine Y,Kostmann C,Bilics V,Simon M,Reményi A,Vincentelli R,Trave G,Nyitray L

更新日期：2019-03-15 00:00:00

abstract：:UL9 protein and ICP8 encoded by the herpes simplex virus type 1 (HSV-1) were shown to catalyze a highly active, non-origin-dependent unwinding of DNA. UL9 protein, the HSV-1 origin binding protein, as a modest helicase activity that is greatly stimulated by the HSV-1 single strand (ss) binding protein, ICP8. Here, ele...

journal_title：Journal of molecular biology

authors： Makhov AM,Boehmer PE,Lehman IR,Griffith JD

更新日期：1996-05-24 00:00:00

abstract：:Domain swapping has been shown to be an important mechanism controlling multiprotein assembly and has been suggested recently as a possible mechanism underlying protein aggregation. Understanding oligomerization via domain swapping is therefore of theoretical and practical importance. By using a symmetrized structure-...

journal_title：Journal of molecular biology

authors： Yang S,Levine H,Onuchic JN

更新日期：2005-09-09 00:00:00

abstract：:The oligomerization and aggregation of the amyloid-beta (A beta) peptide, a cleavage product of the amyloid precursor protein predominantly 40 or 42 amino acids in length, has been implicated in the pathogenesis of Alzheimer's disease. The identification of A beta-binding agents, e.g., antibodies or peptides, constitu...

journal_title：Journal of molecular biology

authors： Hoyer W,Härd T

更新日期：2008-04-25 00:00:00