当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Escherichia coli PriA helicase: fork binding orients the helicase to unwind the lagging strand side of arrested replication forks.

Escherichia coli PriA helicase: fork binding orients the helicase to unwind the lagging strand side of arrested replication forks.

Abstract：

:Escherichia coli PriA is a primosome assembly protein with 3' to 5' helicase activity whose apparent function is to promote resumption of DNA synthesis following replication-fork arrest. Here, we describe how initiation of helicase activity on DNA forks is influenced by both fork structure and by single-strand DNA-binding protein. PriA could recognize and unwind forked substrates where one or both arms were primarily duplex, and PriA required a small (two bases or larger) single-stranded gap at the fork in order to initiate unwinding. The helicase was most active on substrates with a duplex lagging-strand arm and a single-stranded leading-strand arm. On this substrate, PriA was capable of translocating on either the leading or lagging strands to unwind the duplex ahead of the fork or the lagging-strand duplex, respectively. Fork-specific binding apparently orients the helicase domain to unwind the lagging-strand duplex. Binding of single-strand-binding protein to forked templates could inhibit unwinding of the duplex ahead of the fork but not unwinding of the lagging-strand duplex or translocation on the lagging-strand template. While single-strand-binding protein could inhibit binding of PriA to the minimal, unforked DNA substrates, it could not inhibit PriA binding to forked substrates. In the cell, single-strand-binding protein and fork structure may direct PriA helicase to translocate along the lagging-strand template of forked structures such that the primosome is specifically assembled on that DNA strand.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Jones JM,Nakai H

doi

10.1006/jmbi.2001.4930

keywords:

subject

Has Abstract

pub_date

2001-10-05 00:00:00

pages

935-47

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(01)94930-7

journal_volume

312

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Glutamate promotes SSB protein-protein Interactions via intrinsically disordered regions.

    abstract：:E. coli single strand (ss) DNA binding protein (SSB) is an essential protein that binds to ssDNA intermediates formed during genome maintenance. SSB homotetramers bind ssDNA in several modes that differ in occluded site size and cooperativity. High "unlimited" cooperativity is associated with the 35 site size ((SSB)35...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2017.07.021

    authors： Kozlov AG,Shinn MK,Weiland EA,Lohman TM

    更新日期：2017-09-01 00:00:00

  • Structure-guided identification of a laminin binding site on the laminin receptor precursor.

    abstract：:The 37/ 67-kDa human laminin receptor (LamR) is a cell surface receptor for laminin, prion protein, and a variety of viruses. Because of its wide range of ligands, LamR plays a role in numerous pathologies. LamR overexpression correlates with a highly invasive cell phenotype and increased metastatic ability, mediated ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2010.10.028

    authors： Jamieson KV,Hubbard SR,Meruelo D

    更新日期：2011-01-07 00:00:00

  • APOBEC3B and AID have similar nuclear import mechanisms.

    abstract：:Members of the APOBEC (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like) protein family catalyze DNA cytosine deamination and underpin a variety of immune defenses. For instance, several family members, including APOBEC3B (A3B), elicit strong retrotransposon and retrovirus restriction activities. Howev...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.03.011

    authors： Lackey L,Demorest ZL,Land AM,Hultquist JF,Brown WL,Harris RS

    更新日期：2012-06-22 00:00:00

  • Structure of an escape mutant of glycoprotein N2 neuraminidase of influenza virus A/Tokyo/3/67 at 3 A.

    abstract：:The three-dimensional structure of the membrane glycoprotein neuraminidase of an escape mutant of the influenza virus strain A/Tokyo/3/67 has been determined to 3 A (1 A = 0.1 nm) resolution by X-ray diffraction. The mutant virus, selected by growing the virus in the presence of a monoclonal antibody to the neuraminid...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90344-0

    authors： Varghese JN,Webster RG,Laver WG,Colman PM

    更新日期：1988-03-05 00:00:00

  • Sequence analysis of the complete Caenorhabditis elegans myosin heavy chain gene family.

    abstract：:The sequences of three myosin heavy chain (MHC) genes from Caenorhabditis elegans, myo-1, 2 and 3, are presented. These genes, together with unc-54, comprise the entire nematode sacromeric MHC family. Comparison of nematode MHC sequences and sarcomeric, smooth and non-muscle MHCs from other organisms highlights conser...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(89)90229-5

    authors： Dibb NJ,Maruyama IN,Krause M,Karn J

    更新日期：1989-02-05 00:00:00

  • The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus.

    abstract：:Bleomycin (Bm)-binding protein, designated BLMA, which is a Bm resistance determinant from Bm-producing Streptomyces verticillus, was crystallized in a form suitable for X-ray diffraction analysis. The diffraction intensity data were collected up to a resolution of 1.5 A with a merging R-value of 0.054 at a completene...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3404

    authors： Kawano Y,Kumagai T,Muta K,Matoba Y,Davies J,Sugiyama M

    更新日期：2000-01-28 00:00:00

  • Translated translational operator in Escherichia coli. Auto-regulation in the infC-rpmI-rplT operon.

    abstract：:The genes coding for translation initiation factor IF3 (infC) and for the ribosomal proteins L35 (rpmI) and L20 (rplT) are transcribed in that order from a promoter in front of infC. The last two cistrons of the operon (rpmI and rplT) can be transcribed from a weak secondary promoter situated within the first cistron ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(05)80208-6

    authors： Lesage P,Truong HN,Graffe M,Dondon J,Springer M

    更新日期：1990-06-05 00:00:00

  • Topology of the components of the DNA packaging machinery in the phage phi29 prohead.

    abstract：:Chromosome condensation inside dsDNA viral particles is a complex process requiring the coordinated action of several viral components. The similarity of the process in different viral systems has led to the suggestion that there is a common underlying mechanism for DNA packaging, in which the portal vertex or connect...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3712

    authors： Ibarra B,Castón JR,Llorca O,Valle M,Valpuesta JM,Carrascosa JL

    更新日期：2000-05-19 00:00:00

  • ATP synthase from bovine heart mitochondria. In vitro assembly of a stalk complex in the presence of F1-ATPase and in its absence.

    abstract：:Four subunits of the F1F0-ATPase from bovine heart mitochondria have been produced by heterologous over-expression in Escherichia coli. They are the oligomycin sensitivity conferral protein (OSCP), coupling factor 6 (F6) and subunits b and d. Likewise, fragments b', bI, bC, and bM (amino acid residues 79 to 214, 121 t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1591

    authors： Collinson IR,van Raaij MJ,Runswick MJ,Fearnley IM,Skehel JM,Orriss GL,Miroux B,Walker JE

    更新日期：1994-09-30 00:00:00

  • Folding of RNA involves parallel pathways.

    abstract：:Folding kinetics of large RNAs are just beginning to be investigated. We show that the Tetrahymena self-splicing RNA partitions into a population that rapidly reaches the native state, and a slowly folding population that is trapped in metastable misfolded structures. Transitions from the misfolded structures to the n...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1311

    authors： Pan J,Thirumalai D,Woodson SA

    更新日期：1997-10-17 00:00:00

  • HMMSTR: a hidden Markov model for local sequence-structure correlations in proteins.

    abstract：:We describe a hidden Markov model, HMMSTR, for general protein sequence based on the I-sites library of sequence-structure motifs. Unlike the linear hidden Markov models used to model individual protein families, HMMSTR has a highly branched topology and captures recurrent local features of protein sequences and struc...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3837

    authors： Bystroff C,Thorsson V,Baker D

    更新日期：2000-08-04 00:00:00

  • CryptoSite: Expanding the Druggable Proteome by Characterization and Prediction of Cryptic Binding Sites.

    abstract：:Many proteins have small-molecule binding pockets that are not easily detectable in the ligand-free structures. These cryptic sites require a conformational change to become apparent; a cryptic site can therefore be defined as a site that forms a pocket in a holo structure, but not in the apo structure. Because many p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2016.01.029

    authors： Cimermancic P,Weinkam P,Rettenmaier TJ,Bichmann L,Keedy DA,Woldeyes RA,Schneidman-Duhovny D,Demerdash ON,Mitchell JC,Wells JA,Fraser JS,Sali A

    更新日期：2016-02-22 00:00:00

  • Morphology of sheet-like assemblies of pN-collagen, pC-collagen and procollagen studied by scanning transmission electron microscopy mass measurements.

    abstract：:At high concentrations, type I pN-collagen, pC-collagen and procollagen (the first 2 generated from procollagen by enzymic cleavage of C-propeptides and N-propeptides, respectively) can all be made to assemble in vitro into thin D-periodic sheets or tapes. Scanning transmission electron microscopy mass measurements sh...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90385-j

    authors： Holmes DF,Mould AP,Chapman JA

    更新日期：1991-07-05 00:00:00

  • Pulse-chase analysis of the in vivo assembly of the bacteriophage T4 tail.

    abstract：:The in vivo assembly pathway of the complex tail of bacteriophage T4 virus was determined using pulse-chase analysis as a non-invasive alternative to the in vitro experiments previously used to map assembly. Bacteriophage T4 mutants defective in head assembly were used to infect cultures of Escherichia coli in order t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3551

    authors： Ferguson PL,Coombs DH

    更新日期：2000-03-17 00:00:00

  • A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins.

    abstract：:The origin of metal ion selectivity by members of the SmtB/ArsR family of bacterial metal-sensing transcriptional repressors and the mechanism of negative allosteric regulation of DNA binding is poorly understood. Here, we report that two homologous zinc sensors, Staphylococcus aureus CzrA and cyanobacterial SmtB, are...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2003.09.007

    authors： Eicken C,Pennella MA,Chen X,Koshlap KM,VanZile ML,Sacchettini JC,Giedroc DP

    更新日期：2003-10-31 00:00:00

  • Evidence that the KH RNA-binding domains influence the action of the E. coli NusA protein.

    abstract：:The NusA transcription elongation protein, which binds RNA, contains sequences corresponding to the S1 and KH classes of identified RNA binding domains. An essential function in E. coli, NusA is also one of the host factors required for action of the N transcription antitermination protein of lambda. Tandem KH domains...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00238-3

    authors： Zhou Y,Mah TF,Greenblatt J,Friedman DI

    更新日期：2002-05-17 00:00:00

  • Specific tyrosylation of the bulky tRNA-like structure of brome mosaic virus RNA relies solely on identity nucleotides present in its amino acid-accepting domain.

    abstract：:Residues specifying aminoacylation by yeast tyrosyl-tRNA synthetase (TyrRS) of the tRNA-like structure present at the 3'-end of brome mosaic virus (BMV) RNA were determined by the in vitro approach using phage T7 transcripts. They correspond to nucleotides equivalent to base-pair C1-G72 and discriminator base A73 in t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4654

    authors： Fechter P,Giegé R,Rudinger-Thirion J

    更新日期：2001-06-01 00:00:00

  • Studying the Lysine Acetylation of Malate Dehydrogenase.

    abstract：:Protein acetylation plays important roles in many biological processes. Malate dehydrogenase (MDH), a key enzyme in the tricarboxylic acid cycle, has been identified to be acetylated in bacteria by proteomic studies, but no further characterization has been reported. One challenge for studying protein acetylation is t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2017.03.027

    authors： Venkat S,Gregory C,Sturges J,Gan Q,Fan C

    更新日期：2017-05-05 00:00:00

  • Dissecting specificity in the Janus kinases: the structures of JAK-specific inhibitors complexed to the JAK1 and JAK2 protein tyrosine kinase domains.

    abstract：:The Janus kinases (JAKs) are a pivotal family of protein tyrosine kinases (PTKs) that play prominent roles in numerous cytokine signaling pathways, with aberrant JAK activity associated with a variety of hematopoietic malignancies, cardiovascular diseases and immune-related disorders. Whereas the structures of the JAK...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.01.041

    authors： Williams NK,Bamert RS,Patel O,Wang C,Walden PM,Wilks AF,Fantino E,Rossjohn J,Lucet IS

    更新日期：2009-03-20 00:00:00

  • For Better or Worse: Cytosolic DNA Sensing during Intracellular Bacterial Infection Induces Potent Innate Immune Responses.

    abstract：:Many intracellular bacterial pathogens previously thought to remain sealed within a vacuole can be recognized by cytosolic innate immune sensors. While a wide array of cytosolic nucleic acid sensors have been characterized in the context of viral infection, we are only now beginning to examine how these same molecules...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2016.04.030

    authors： Patrick KL,Bell SL,Watson RO

    更新日期：2016-08-28 00:00:00

  • Torque-speed relationships of Na+-driven chimeric flagellar motors in Escherichia coli.

    abstract：:The bacterial flagellar motor is a rotary motor in the cell envelope of bacteria that couples ion flow across the cytoplasmic membrane to torque generation by independent stators anchored to the cell wall. The recent observation of stepwise rotation of a Na(+)-driven chimeric motor in Escherichia coli promises to reve...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2007.12.023

    authors： Inoue Y,Lo CJ,Fukuoka H,Takahashi H,Sowa Y,Pilizota T,Wadhams GH,Homma M,Berry RM,Ishijima A

    更新日期：2008-03-07 00:00:00

  • Both N-Terminal and C-Terminal Histidine Residues of the Prion Protein Are Essential for Copper Coordination and Neuroprotective Self-Regulation.

    abstract：:The cellular prion protein (PrPC) comprises two domains: a globular C-terminal domain and an unstructured N-terminal domain. Recently, copper has been observed to drive tertiary contact in PrPC, inducing a neuroprotective cis interaction that structurally links the protein's two domains. The location of this interacti...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.05.020

    authors： Schilling KM,Tao L,Wu B,Kiblen JTM,Ubilla-Rodriguez NC,Pushie MJ,Britt RD,Roseman GP,Harris DA,Millhauser GL

    更新日期：2020-07-24 00:00:00

  • Interstrand duplexes in Friend erythroleukemia nuclear RNA. The interaction of non-polyadenylated nuclear RNA with polyadenylated nuclear RNA and with small nuclear RNAs.

    abstract：:Intermolecular duplexes among large nuclear RNAs, and between small nuclear RNA and heterogeneous nuclear RNA, were studied after isolation by a procedure that yielded protein-free RNA without the use of phenol or high salt. The bulk of the pulse-labeled RNA had a sedimentation coefficient greater than 45 S. After hea...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90461-3

    authors： Miller KS,Zbrzezna V,Pogo AO

    更新日期：1984-08-05 00:00:00

  • Sequence and membrane determinants of the random coil-helix transition of α-synuclein.

    abstract：:A random coil-helix transition underlies the association of the presynaptic protein α-synuclein (αS) with curved vesicle membranes to fold Asp2-Ala89 into a continuous helix. To clarify this transition, we examined αS folding cooperativity, helix nucleation and propagation in relation to membrane stabilization and lea...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2014.02.024

    authors： Lokappa SB,Suk JE,Balasubramanian A,Samanta S,Situ AJ,Ulmer TS

    更新日期：2014-05-15 00:00:00

  • Identifying Transcription Error-Enriched Genomic Loci Using Nuclear Run-on Circular-Sequencing Coupled with Background Error Modeling.

    abstract：:RNA polymerase transcribes certain genomic loci with higher errors rates. These transcription error-enriched genomic loci (TEELs) have implications in disease. Current deep-sequencing methods cannot distinguish TEELs from post-transcriptional modifications, stochastic transcription errors, and technical noise, impedin...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2020.04.011

    authors： Cheung PP,Jiang B,Booth GT,Chong TH,Unarta IC,Wang Y,Suarez GD,Wang J,Lis JT,Huang X

    更新日期：2020-06-12 00:00:00

  • Further sequence analysis of the phage lambda receptor site. Possible implications for the organization of the lamB protein in Escherichia coli K12.

    abstract：:We present the DNA sequence alterations due to seven lamB missense mutations yielding resistance to phages lambda and K10. They reveal five different amino acid positions in the LamB protein. Three positions (245, 247 and 249) define a new region required for phage adsorption. The two other positions (148 and 152) bel...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90355-3

    authors： Charbit A,Clement JM,Hofnung M

    更新日期：1984-05-25 00:00:00

  • Structural characterization of intramolecular Hg(2+) transfer between flexibly linked domains of mercuric ion reductase.

    abstract：:The enzyme mercuric ion reductase MerA is the central component of bacterial mercury resistance encoded by the mer operon. Many MerA proteins possess metallochaperone-like N-terminal domains (NmerA) that can transfer Hg(2+) to the catalytic core domain (Core) for reduction to Hg(0). These domains are tethered to the h...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2011.08.042

    authors： Johs A,Harwood IM,Parks JM,Nauss RE,Smith JC,Liang L,Miller SM

    更新日期：2011-10-28 00:00:00

  • Methylation of the promoter region may be involved in tissue-specific expression of the mouse terminal deoxynucleotidyl transferase gene.

    abstract：:The terminal deoxynucleotidyl transferase gene (TdT) is expressed in mice only in early B and T lymphoid precursors a few days after birth. Transactivating factors have been shown to contribute to the lymphoid specific expression of TdT, but they do not account entirely for the restriction of its expression to early p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1999.3079

    authors： Nourrit F,Coquilleau I,D'Andon MF,Rougeon F,Doyen N

    更新日期：1999-09-17 00:00:00

  • Identification of a specific motif of the DSS1 protein required for proteasome interaction and p53 protein degradation.

    abstract：:Deleted in Split hand/Split foot 1 (DSS1) was previously identified as a novel 12-O-tetradecanoylphorbol-13-acetate (TPA)-inducible gene with possible involvement in early event of mouse skin carcinogenesis. The mechanisms by which human DSS1 (HsDSS1) exerts its biological effects via regulation of the ubiquitin-prote...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.08.044

    authors： Wei SJ,Williams JG,Dang H,Darden TA,Betz BL,Humble MM,Chang FM,Trempus CS,Johnson K,Cannon RE,Tennant RW

    更新日期：2008-11-14 00:00:00

  • Fibrinogen structure in projection at 18 A resolution. Electron density by co-ordinated cryo-electron microscopy and X-ray crystallography.

    abstract：:Electron microscope images of frozen-hydrated crystals of a proteolytically modified fibrinogen show excellent preservation of the structure. An electron density map of the key centric projection of the crystal at 18 A resolution has been obtained by combining the phases derived from cryo-electron microscopy with X-ra...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90739-s

    authors： Rao SP,Poojary MD,Elliott BW Jr,Melanson LA,Oriel B,Cohen C

    更新日期：1991-11-05 00:00:00