Specific tyrosylation of the bulky tRNA-like structure of brome mosaic virus RNA relies solely on identity nucleotides present in its amino acid-accepting domain.

abstract：:The terminal regions of monomeric hook protein from Salmonella typhimurium are known to be highly mobile and exposed to the solvent. Although hook protein exhibits an unusual far-UV circular dichroism spectrum, resembling that of random coil structures, our calorimetric experiments clearly demonstrate that the molecul...

journal_title：Journal of molecular biology

authors： Vonderviszt F,Závodszky P,Ishimura M,Uedaira H,Namba K

更新日期：1995-08-25 00:00:00

abstract：:The Ser/Thr kinase CK2 (former name: casein kinase 2) is a heterotetrameric enzyme composed of two catalytic chains (CK2alpha) attached to a dimer of noncatalytic subunits. Together with the cyclin-dependent kinases and the mitogen-activated protein kinases, CK2alpha belongs to the CMGC family of the eukaryotic protei...

journal_title：Journal of molecular biology

authors： Raaf J,Klopffleisch K,Issinger OG,Niefind K

更新日期：2008-03-14 00:00:00

abstract：:The development of drug resistance has long plagued our efforts to curtail viral infections in general and influenza in particular. The problem is particularly challenging since the exact mode of resistance may be difficult to predict, without waiting for untreatable strains to evolve. Herein, a different approach is ...

journal_title：Journal of molecular biology

authors： Assa D,Alhadeff R,Krugliak M,Arkin IT

更新日期：2016-10-09 00:00:00

abstract：:The preferred DNase I cleavage sites within the 160 bp tyrT DNA fragment were identified by studying the initial rate of cleavage of individual bonds. The results show that there is no correlation between the rate of cleavage and the identity of the dinucleotide sequence that is cleaved. Examination of the sequences s...

journal_title：Journal of molecular biology

authors： Herrera JE,Chaires JB

更新日期：1994-02-18 00:00:00

abstract：:A novel gene from the nematode Caenorhabditis elegans was isolated by hybridization with a human calmodulin complementary DNA probe. This gene, cal-1, is present at one copy per haploid genome. In-situ hybridization of the cloned gene to metaphase chromosomes allowed us to assign it to the nematode linkage group IV. T...

journal_title：Journal of molecular biology

authors： Salvato M,Sulston J,Albertson D,Brenner S

更新日期：1986-08-05 00:00:00

abstract：:The movement of the bacterial insertion sequence IS1 often generates cointegrate structures in which donor and target replicons are connected by direct repeats of IS1. The experiments reported here were designed to understand how IS1 transposition is controlled. Our physical characterization of the structures of coint...

journal_title：Journal of molecular biology

authors： Biel SW,Adelt G,Berg DE

更新日期：1984-04-05 00:00:00

abstract：:RIG-I-like receptors (RLRs) are nucleic acid sensors that activate antiviral innate immune response. These molecules recognize diverse non-self RNA substrates and are antagonized by several viral inhibitors. We performed an evolutionary analysis of RLR genes (RIG-I, MDA5, and LGP2) in mammals. Results indicated that p...

journal_title：Journal of molecular biology

authors： Cagliani R,Forni D,Tresoldi C,Pozzoli U,Filippi G,Rainone V,De Gioia L,Clerici M,Sironi M

更新日期：2014-03-20 00:00:00

abstract：:Single crystals of ribonuclease I from Escherichia coli have been obtained by the vapor diffusion method. The crystals belong to the tetragonal space group P4(1)2(1)2 or its enantiomer P4(3)2(1)2 and have cell parameters a = b = 119.01 A and c = 34.40 A. There is one 27,000 dalton monomer in the asymmetric unit. The c...

journal_title：Journal of molecular biology

authors： Lim LW,Mathur S,Cannistraro VJ,Kennell D

更新日期：1993-11-20 00:00:00

abstract：:The structure of Mn(III) superoxide dismutase (Mn(III)SOD) from Thermus thermophilus, a tetramer of chains 203 residues in length, has been refined by restrained least-squares methods. The R-factor [formula: see text] for the 54,056 unique reflections measured between 10.0 and 1.8 A (96% of all possible reflections) i...

journal_title：Journal of molecular biology

authors： Ludwig ML,Metzger AL,Pattridge KA,Stallings WC

更新日期：1991-05-20 00:00:00

abstract：:Peptides that bind to major histocompatibility complex products (MHC) are known to exhibit certain sequence motifs which, though common, are neither necessary nor sufficient for binding: MHCs bind certain peptides that do not have the characteristic motifs and only about 30% of the peptides having the required motif, ...

journal_title：Journal of molecular biology

authors： Gulukota K,Sidney J,Sette A,DeLisi C

更新日期：1997-04-18 00:00:00

abstract：:Nucleocapsid assembly is an essential process in the replication of the non-segmented, negative-sense RNA viruses (NNVs). Unassembled nucleoprotein (N(0)) is maintained in an RNA-free and monomeric form by its viral chaperone, the phosphoprotein (P), forming the N(0)-P complex. Our earlier work solved the structure of...

journal_title：Journal of molecular biology

authors： Yabukarski F,Leyrat C,Martinez N,Communie G,Ivanov I,Ribeiro EA Jr,Buisson M,Gerard FC,Bourhis JM,Jensen MR,Bernadó P,Blackledge M,Jamin M

更新日期：2016-07-03 00:00:00

abstract：:We improved a motility assay system by using an affinity-purified antibody against the C-terminal globular domain of characean myosin. This improvement allowed us to study the sensitivity to ionic strength or the processivity of characean myosin. The sliding velocity of actin filaments on a characean myosin-coated sur...

journal_title：Journal of molecular biology

authors： Awata JY,Kashiyama T,Ito K,Yamamoto K

更新日期：2003-02-21 00:00:00

abstract：:The molten globule has been assumed to be a major intermediate state of protein folding. To extend our understanding of protein folding it is important to elucidate the thermodynamic mechanism of conformational stability of the molten globule. To clarify the role of electrostatic charge repulsion in the stability of t...

journal_title：Journal of molecular biology

authors： Goto Y,Nishikiori S

更新日期：1991-12-05 00:00:00

abstract：:Group II intron retrohoming occurs by a mechanism in which the intron RNA reverse splices directly into one strand of a double-stranded DNA target site, while the intron-encoded reverse transcriptase uses a C-terminal DNA endonuclease activity to cleave the opposite strand and then uses the cleaved 3' end as a primer ...

journal_title：Journal of molecular biology

authors： San Filippo J,Lambowitz AM

更新日期：2002-12-13 00:00:00

abstract：:We have used lacZ reporter genes to assess leftward ribosome frameshifting on sequences containing the quadruplet U UUC followed by several different triplets coding for lysine, isoleucine, or leucine. Limitation for lysine-tRNA provokes leftward frameshifting when the slippery quadruplet is followed by either lysine ...

journal_title：Journal of molecular biology

authors： Barak Z,Lindsley D,Gallant J

更新日期：1996-03-08 00:00:00

abstract：:The position of the tertiary Levitt pair between nucleotides 15 and 48 in the transfer RNA core region suggests a key role in stabilizing the joining of the two helical domains, and in maintaining the relative orientations of the D and variable loops. E. coli tRNA(Gln) possesses the canonical Pu15-Py48 trans pairing a...

journal_title：Journal of molecular biology

authors： Sherlin LD,Bullock TL,Newberry KJ,Lipman RS,Hou YM,Beijer B,Sproat BS,Perona JJ

更新日期：2000-06-02 00:00:00

abstract：:Initiation of transcription at malEp and malKp, two divergent Escherichia coli promoters, depends on the presence of both CRP, a pleiotropic activator, and MalT, the maltose regulon activator. We carried out in vivo genetic and functional analysis of these promoters and characterized their interaction with MalT and CR...

journal_title：Journal of molecular biology

authors： Raibaud O,Vidal-Ingigliardi D,Richet E

更新日期：1989-02-05 00:00:00

abstract：:The proteins coded by the five major capsid genes of herpes simplex virus 1, VP5 (gene UL19), VP19c (UL38), VP23 (UL18), pre-VP22a (UL26.5), and pre-VP21 (UL26), assemble into fragile roundish "procapsids", which mature into robust polyhedral capsids in a transition similar to that undergone by bacteriophage proheads....

journal_title：Journal of molecular biology

authors： Trus BL,Booy FP,Newcomb WW,Brown JC,Homa FL,Thomsen DR,Steven AC

更新日期：1996-11-01 00:00:00

abstract：:A library of variant enzymes was created by combined shuffling of the DNA encoding the human Mu class glutathione transferases GST M1-1 and GST M2-2. The parental GSTs are 84 % sequence identical at the protein level, but their specific activities with the substrates aminochrome and 2-cyano-1,3-dimethyl-1-nitrosoguani...

journal_title：Journal of molecular biology

authors： Hansson LO,Bolton-Grob R,Massoud T,Mannervik B

更新日期：1999-03-26 00:00:00

abstract：:An analogue of the important folding intermediate of BPTI with only the disulphide bond between Cys30 and Cys51 has been characterized by 1H and 15N NMR techniques. In particular, the dynamics of the polypeptide backbone were characterized using (1H)-15N NOE and 15N T1 and T2 relaxation data. The intermediate is parti...

journal_title：Journal of molecular biology

authors： van Mierlo CP,Darby NJ,Keeler J,Neuhaus D,Creighton TE

更新日期：1993-02-20 00:00:00

abstract：:The acid and base catalytic rate constants, kH, obs and kOH, obs and the pH at the minimum rate, pHmin, of 25 rapidly exchanging protons in bovine pancreatic trypsin inhibitor have been determined. Here we report the labeling procedure giving 1H nuclear magnetic resonance spectral resolution of seven additional rapidl...

journal_title：Journal of molecular biology

authors： Tüchsen E,Woodward C

更新日期：1987-02-20 00:00:00

abstract：:Cytokines orchestrate responses to pathogens and in inflammatory processes, but they also play an important role in cancer by shaping the expression levels of cytokine response genes. Here, we conducted a large profiling study comparing miRNome and mRNA transcriptome data generated following different cytokine stimula...

journal_title：Journal of molecular biology

authors： Kirchmeyer M,Servais F,Ginolhac A,Nazarov PV,Margue C,Philippidou D,Nicot N,Behrmann I,Haan C,Kreis S

更新日期：2020-11-06 00:00:00

abstract：:Prion diseases are neurodegenerative disorders caused by the misfolding of the cellular prion protein (PrPC). Gerstmann-Sträussler-Scheinker syndrome is an inherited prion disease with one early-onset allele (HRdup) containing an eight-amino-acid insertion; this LGGLGGYV insert is positioned after valine 129 (human Pr...

journal_title：Journal of molecular biology

authors： Fu ZL,Holmes PC,Westaway D,Sykes BD

更新日期：2019-06-28 00:00:00

abstract：:During general genetic recombination and recombinational DNA repair, DNA damages and heterologies are often encountered which must be efficiently processed by the cellular recombination machinery. In RecA-mediated three-strand exchange reactions between single-stranded circular and linear duplex DNA, or four-strand ex...

journal_title：Journal of molecular biology

authors： Adams DE,West SC

更新日期：1996-11-08 00:00:00

abstract：:Current data suggest that globular domains may form amyloids via different mechanisms. Nevertheless, there are indications that the initiation of the process takes place invariably in the less stable segments of a protein domain. We have studied the sequence and structural conservation of beta(2)-microglobulin that de...

journal_title：Journal of molecular biology

authors： Benyamini H,Gunasekaran K,Wolfson H,Nussinov R

更新日期：2003-06-27 00:00:00

abstract：:A 1.9 A resolution X-ray structure of the apo-form of Corynebacterium 2,5-diketo-d-gluconic acid reductase A (2,5-DKGR A), a member of the aldo-keto reductase superfamily, has been determined by molecular replacement using the NADPH-bound form of the same enzyme as the search model. 2,5-DKGR A catalyzes the NADPH-depe...

journal_title：Journal of molecular biology

authors： Sanli G,Blaber M

更新日期：2001-06-22 00:00:00

abstract：:We report the first protein phase-diagram characterized by a combination of volumetric, calorimetric, and spectroscopic techniques. More specifically, we use ultrasonic velocimetry, densimetry, and differential scanning calorimetry, in conjunction with UV absorbance and CD spectroscopy to detect and to characterize th...

journal_title：Journal of molecular biology

authors： Chalikian TV,Völker J,Anafi D,Breslauer KJ

更新日期：1997-11-28 00:00:00

abstract：:Intermolecular duplexes among large nuclear RNAs, and between small nuclear RNA and heterogeneous nuclear RNA, were studied after isolation by a procedure that yielded protein-free RNA without the use of phenol or high salt. The bulk of the pulse-labeled RNA had a sedimentation coefficient greater than 45 S. After hea...

journal_title：Journal of molecular biology

authors： Miller KS,Zbrzezna V,Pogo AO

更新日期：1984-08-05 00:00:00

abstract：:Filamentous bacteriophage M13 is a single-stranded DNA phage about 65 A in diameter and 9300 A long. X-ray diffraction studies of magnetically oriented fibers of native, mercury and iodine-labeled phage particles have been used to determine the arrangement of the major coat protein, the gene 8 product, in the virion. ...

journal_title：Journal of molecular biology

authors： Glucksman MJ,Bhattacharjee S,Makowski L

更新日期：1992-07-20 00:00:00

abstract：:ETV6 (or TEL), a transcriptional repressor belonging to the ETS family, is frequently involved in chromosomal translocations linked with human cancers. It displays a DNA-binding mode distinct from other ETS proteins due to the presence of a self-associating PNT domain. In this study, we used NMR spectroscopy to dissec...

journal_title：Journal of molecular biology

authors： Coyne HJ 3rd,De S,Okon M,Green SM,Bhachech N,Graves BJ,McIntosh LP

更新日期：2012-08-03 00:00:00