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A complex nucleoprotein structure involved in activation of transcription of two divergent Escherichia coli promoters.

Abstract：

:Initiation of transcription at malEp and malKp, two divergent Escherichia coli promoters, depends on the presence of both CRP, a pleiotropic activator, and MalT, the maltose regulon activator. We carried out in vivo genetic and functional analysis of these promoters and characterized their interaction with MalT and CRP using DNase I footprinting. The functional limits of the promoters are located about 240 base-pairs (bp) upstream of their transcription start sites, which are 271 bp apart. These promoters therefore overlap by about 210 bp. The overlapping region encompasses four CRP-binding sites and at least four MalT-binding sites. Insertions in the centre of this region are tolerated provided that they correspond to an integral number of DNA helix turns. In DNase I footprinting experiments performed on the complex formed by MalT with malEp-malKp, the DNA appears to be wrapped around the protein. We propose a model for the nucleoprotein structure that might be involved in transcription activation at these divergent promoters.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Raibaud O,Vidal-Ingigliardi D,Richet E

doi

10.1016/0022-2836(89)90218-0

subject

Has Abstract

pub_date

1989-02-05 00:00:00

pages

471-85

issue

3

eissn

0022-2836

issn

1089-8638

pii

0022-2836(89)90218-0

journal_volume

205

pub_type

杂志文章

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