Abstract:
:Cytokines orchestrate responses to pathogens and in inflammatory processes, but they also play an important role in cancer by shaping the expression levels of cytokine response genes. Here, we conducted a large profiling study comparing miRNome and mRNA transcriptome data generated following different cytokine stimulations. Transcriptomic responses to STAT1- (IFNγ, IL-27) and STAT3-activating cytokines (IL6, OSM) were systematically compared in nine cancerous and non-neoplastic cell lines of different tissue origins (skin, liver and colon). The largest variation in our datasets was seen between cell lines of the three different tissues rather than stimuli. Notably, the variability in miRNome datasets was a lot more pronounced than in mRNA data. Our data also revealed that cells of skin, liver and colon tissues respond very differently to cytokines and that the cell signaling networks activated or silenced in response to STAT1- or STAT3-activating cytokines are specific to the tissue and the type of cytokine. However, globally, STAT1-activating cytokines had stronger effects than STAT3-inducing cytokines with most significant responses in liver cells, showing more genes upregulated and with higher fold change. A more detailed analysis of gene regulations upon cytokine stimulation in these cells provided insights into STAT1- versus STAT3-driven processes in hepatocarcinogenesis. Finally, independent component analysis revealed interconnected transcriptional networks distinct between cancer cells and their healthy counterparts.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Kirchmeyer M,Servais F,Ginolhac A,Nazarov PV,Margue C,Philippidou D,Nicot N,Behrmann I,Haan C,Kreis Sdoi
10.1016/j.jmb.2020.09.011subject
Has Abstractpub_date
2020-11-06 00:00:00pages
5902-5919issue
22eissn
0022-2836issn
1089-8638pii
S0022-2836(20)30553-2journal_volume
432pub_type
杂志文章abstract::Crystals of the mouse major urinary protein (MUP) and rat alpha-2u globulin (AMG) have been grown from solutions of polyethylene glycol 3350 and CdCl2, respectively. The crystals differ both in their morphologies and space groups but have very similar unit cell sizes. AMG crystallized in P2(1) (a = 56.6 A, b = 103.8 A...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/0022-2836(91)90258-8
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abstract::The amphipathic alpha-helix is a recurrent feature of membrane-active proteins, peptides, and toxins. Despite extensive biophysical studies, the structural details of its affinity for membrane interfaces remain rather vague. We report here the first results of an effort to obtain detailed structural information about ...
journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:2011-09-09 00:00:00
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.jmb.2016.02.016
更新日期:2016-03-27 00:00:00
abstract::The type III secretion system (T3SS) is essential in the pathogenesis of Yersinia pestis, the causative agent of plague. A small protein, LcrG, functions as a chaperone to the tip protein LcrV, and the LcrG-LcrV interaction is important in regulating protein secretion through the T3SS. The atomic structure of the LcrG...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/j.jmb.2015.07.024
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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abstract::Cholera toxin (CT) holotoxin must be activated to intoxicate host cells. This process requires the intracellular dissociation of the enzymatic CTA1 domain from the holotoxin components CTA2 and B5, followed by subsequent interaction with the host factor ADP ribosylation factor 6 (ARF6)-GTP. We report the first NMR-bas...
journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
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abstract::A highly thermostable xylanase isolated from the thermophilic fungus Paecilomyces varioti has been crystallized by the vapour diffusion method. The isolation of this enzyme by crystallization directly from the culture filtrate projects this fungus as an important source for large-scale production of pure xylanase. The...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/0022-2836(94)90052-3
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.2000.4393
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:2000-01-28 00:00:00