Evidence for helical unwinding of an RNA substrate by the RNA enzyme RNase P: use of an interstrand disulfide crosslink in substrate.

abstract：:The antibiotic thiostrepton, a thiazole-containing peptide, inhibits translation and ribosomal GTPase activity by binding directly to a limited and highly conserved region of the large subunit ribosomal RNA termed the GTPase center. We have previously used a filter binding assay to examine the binding of ribosomal pro...

journal_title：Journal of molecular biology

authors： Ryan PC,Lu M,Draper DE

更新日期：1991-10-20 00:00:00

abstract：:We describe a hybrid statistical mechanical and dynamical approach for modelling the formation of closed, open and elongating complexes of RNA polymerase, the interactions of these polymerases to produce transcriptional interference, and the regulation of these processes by a DNA-binding and DNA-looping regulatory pro...

journal_title：Journal of molecular biology

authors： Dodd IB,Shearwin KE,Sneppen K

更新日期：2007-06-22 00:00:00

abstract：:The production of recombinant embryonic haemoglobins via a yeast expression system has enabled structural and functional studies to be conducted on these proteins. As part of a programme aimed at understanding the properties of the embryonic haemoglobins we have crystallized the human alpha2 epsilon2 (Gower II) embryo...

journal_title：Journal of molecular biology

authors： Sutherland-Smith AJ,Baker HM,Hofmann OM,Brittain T,Baker EN

更新日期：1998-07-17 00:00:00

abstract：:Reconstitution of liver fluke (Dicrocoelium dendriticum) apo-hemoglobin with hemins selectively deuterated at specific positions has permitted the assignment of several heme resonances in the proton nuclear magnetic resonance spectrum of the Met-aquo and Met-cyano forms of the holoprotein. It was established that in t...

journal_title：Journal of molecular biology

authors： Lecomte JT,La Mar GN,Smit JD,Winterhalter KH,Smith KM,Langry KC,Leung HK

更新日期：1987-09-05 00:00:00

abstract：:The molecular chaperone Hsp90 and its cofactor Cdc37 are required for the stability of protein kinases in the cellular environment. Upon pharmacological inhibition of Hsp90, the Hsp90-dependent kinases are degraded quickly by the proteasome. Clear physiological evidence for the formation of heterooligomeric complexes ...

journal_title：Journal of molecular biology

authors： Eckl JM,Daake M,Schwartz S,Richter K

更新日期：2016-10-09 00:00:00

abstract：:Heat capacity curves as obtained from differential scanning calorimetry are an outstanding source for molecular information on protein folding and ligand-binding energetics. However, deconvolution of C(p) data of proteins in the presence of ligands can be compromised by indeterminacies concerning the correct choice of...

journal_title：Journal of molecular biology

authors： Rösgen J,Hinz HJ

更新日期：2001-03-02 00:00:00

abstract：:The RhaS and RhaR regulatory proteins are encoded in the Escherichia coli L-rhamnose gene cluster. We used complementation analysis and DNA mobility shift assays to show that RhaR is not the direct activator of the L-rhamnose catabolic operon, rhaBAD. An in-frame deletion of rhaS (rhaS-rhaR+) eliminated expression fro...

journal_title：Journal of molecular biology

authors： Egan SM,Schleif RF

更新日期：1993-11-05 00:00:00

abstract：:Bacteriophage T7 DNA replication is initiated at a site 15% of the distance from the genetic left end of the chromosome. This primary origin contains two tandem T7 RNA polymerase promoters (phi 1.1A and phi 1.1B) followed by an A + T-rich region. When the primary origin region is deleted replication initiates at secon...

journal_title：Journal of molecular biology

authors： Rabkin SD,Richardson CC

更新日期：1988-12-20 00:00:00

abstract：:Two major methods are currently being used to characterize transient intermediates during protein folding at the level of individual residues. Nuclear magnetic resonance (n.m.r.) measurements on the protection of peptide NH hydrogens against exchange with solvent during refolding can provide information about secondar...

journal_title：Journal of molecular biology

authors： Matouschek A,Serrano L,Meiering EM,Bycroft M,Fersht AR

更新日期：1992-04-05 00:00:00

abstract：:The chicken nuclear lamina is composed of at least three proteins called lamins A, B1 and B2. In addition, putative precursors are transiently expressed during in vivo synthesis of lamins A and B2. Here we report the complete sequence of lamin B2 as it is deduced from a cloned cDNA. Comparison of lamin B2 with lamins ...

journal_title：Journal of molecular biology

authors： Vorburger K,Lehner CF,Kitten GT,Eppenberger HM,Nigg EA

更新日期：1989-08-05 00:00:00

abstract：:phi 6 is an enveloped dsRNA bacterial virus. Its segmented genome resides inside the virion associated polymerase complex which is formed by four proteins (P1, P2, P4 and P7) encoded by the viral L segment. Complete and incomplete polymerase complex particles can be produced using cDNA copies of this largest genome se...

journal_title：Journal of molecular biology

authors： Juuti JT,Bamford DH

更新日期：1995-06-09 00:00:00

abstract：:The crystal structure of glycosyltrehalose trehalohydrolase from the hyperthermophilic archaeum Sulfolobus solfataricus KM1 has been solved by multiple isomorphous replacement. The enzyme is an alpha-amylase (family 13) with unique exo-amylolytic activity for glycosyltrehalosides. It cleaves the alpha-1,4 glycosidic b...

journal_title：Journal of molecular biology

authors： Feese MD,Kato Y,Tamada T,Kato M,Komeda T,Miura Y,Hirose M,Hondo K,Kobayashi K,Kuroki R

更新日期：2000-08-11 00:00:00

abstract：:A repetitive DNA sequence family from Xenopus laevis that has an unusual genomic organization has been identified. It has been shown by blot-hybridization that this sequence occurs in clusters containing variable numbers of the 388 base-pair repeating unit. There are approximately 500 such clusters in the genome, and ...

journal_title：Journal of molecular biology

authors： Lam BS,Carroll D

更新日期：1983-04-25 00:00:00

abstract：:Enoyl-[acyl carrier protein] (ACP) reductase (ENR) is a key enzyme in type II fatty acid synthesis that catalyzes the last step in each elongation cycle. Therefore, it has been considered as a target for antibiotics. However, recent studies indicate that some pathogens have more than one ENR; in particular, Bacillus s...

journal_title：Journal of molecular biology

authors： Kim KH,Ha BH,Kim SJ,Hong SK,Hwang KY,Kim EE

更新日期：2011-02-25 00:00:00

abstract：:Dof is a large molecule essential for signal transduction by the two FGF receptors in Drosophila. It contains two ankyrin repeats and a coiled-coil region, but has no other recognisable structural motif. Dof shares these features with its closest vertebrate relatives, the B-cell signalling molecules BCAP and BANK. In ...

journal_title：Journal of molecular biology

authors： Battersby A,Csiszár A,Leptin M,Wilson R

更新日期：2003-06-06 00:00:00

abstract：:The process of heat denaturation of recombinant factor XIII (rFXIII), as well as its C-terminal 24 kDA and 12 kDa elastase-produced fragments starting at Ser514 and Thr628, respectively, was investigated in a wide range of conditions by fluorescence, CD and differential scanning calorimetry (DSC). It was found that th...

journal_title：Journal of molecular biology

authors： Kurochkin IV,Procyk R,Bishop PD,Yee VC,Teller DC,Ingham KC,Medved LV

更新日期：1995-04-28 00:00:00

abstract：:Nicotine is the principal addictive component of tobacco. Blocking its passage from the lung to the brain with nicotine-specific antibodies is a promising approach for the treatment of smoking addiction. We have determined the crystal structure of nicotine bound to the Fab fragment of a fully human monoclonal antibody...

journal_title：Journal of molecular biology

authors： Tars K,Kotelovica S,Lipowsky G,Bauer M,Beerli RR,Bachmann MF,Maurer P

更新日期：2012-01-06 00:00:00

abstract：:Signal transduction pathways mediated by cyclic-bis(3'→5')-dimeric GMP (c-di-GMP) control many important and complex behaviors in bacteria. C-di-GMP is synthesized through the action of GGDEF domains that possess diguanylate cyclase activity and is degraded by EAL or HD-GYP domains with phosphodiesterase activity. The...

journal_title：Journal of molecular biology

authors： Guzzo CR,Dunger G,Salinas RK,Farah CS

更新日期：2013-06-26 00:00:00

abstract：:DNA polymerases that initiate replication by protein-priming are able to catalyze terminal protein (TP)-primed initiation, the following transition steps and finally DNA-primed elongation. Therefore, their structures must be able to position sequentially both primers, TP and DNA, at a common binding site. For DNA-temp...

journal_title：Journal of molecular biology

authors： Truniger V,Lázaro JM,Blanco L,Salas M

更新日期：2002-04-19 00:00:00

abstract：:Rhodobacter sphaeroides rpoE encodes a 19.2 kDa protein, sigma(E), related to members of the extra-cytoplasmic function subfamily of eubacterial RNA polymerase sigma factors. We demonstrate that sigma(E) directs transcription from rpoE P1, the promoter for the rpoEchrR operon, and from cycA P3, a promoter for the cyto...

journal_title：Journal of molecular biology

authors： Newman JD,Falkowski MJ,Schilke BA,Anthony LC,Donohue TJ

更新日期：1999-11-26 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV, or HHV-8) encodes a pathogenic viral homologue of human interleukin-6 (IL-6). In contrast to human IL-6 (hIL-6), viral IL-6 (vIL-6) binds directly to, and activates, the shared human cytokine signaling receptor gp130 without the requirement for pre-complexation to a speci...

journal_title：Journal of molecular biology

authors： Boulanger MJ,Chow DC,Brevnova E,Martick M,Sandford G,Nicholas J,Garcia KC

更新日期：2004-01-09 00:00:00

abstract：:Trichorzianine A 1 is one of the main components of a mixture of related antibiotic peptides (trichorzianines) produced by the fungus Trichoderma harzianum. Good crystals were obtained and allowed X-ray diffraction up to 0.8 A resolution. The space group is orthorhombic, C222(1), Z = 8, a = 64.8 (1) A, b = 9.33 (3) A,...

journal_title：Journal of molecular biology

authors： Bachet B,Brassy C,Morize I,Surcouf E,Mornon JP,Bodo B,Rebuffat S

更新日期：1983-11-05 00:00:00

abstract：:As enzymes evolve and diverge from common ancestor sequences, they often keep their overall reaction chemistry but specialize in the binding of different cognate ligands. This study borrows methods for the computational assessment of 2D similarity of small molecules from the field of chemoinformatics, to examine the e...

journal_title：Journal of molecular biology

authors： Nobeli I,Spriggs RV,George RA,Thornton JM

更新日期：2005-03-25 00:00:00

abstract：:In humans, NKG2D is an activating receptor on natural killer (NK) cells and a costimulatory receptor on certain T cells and plays a central role in mediating immune responses in autoimmune diseases, infectious diseases, and cancer. Monoclonal antibodies that antagonize or agonize immune responses mediated by human NKG...

journal_title：Journal of molecular biology

authors： Kwong KY,Baskar S,Zhang H,Mackall CL,Rader C

更新日期：2008-12-31 00:00:00

abstract：:Native state 1H NMR resonance assignments for 125 of the 129 residues of equine lysozyme have enabled measurement of the hydrogen exchange kinetics for over 60 backbone amide and three tryptophan indole hydrogen atoms in the native state. Native holo equine lysozyme hydrogen exchange protection factors are as large as...

journal_title：Journal of molecular biology

authors： Morozova-Roche LA,Arico-Muendel CC,Haynie DT,Emelyanenko VI,Van Dael H,Dobson CM

更新日期：1997-05-23 00:00:00

abstract：:Phenylalanine hydroxylase from rat liver has been crystallized from polyethylene glycol 4500 with sodium formate. The crystals are tetragonal rods and belong to space group P4(1)22 or P4(3)22 with unit cell dimensions a = b = 57.6 A and c = 304.1 A. They diffract to at least 2.4 A resolution and have one molecule per ...

journal_title：Journal of molecular biology

authors： Celikel R,Davis MD,Dai XP,Kaufman S,Xuong NH

更新日期：1991-04-05 00:00:00

abstract：:Crystals of the catalytic domain of human fibroblast collagenase have been grown in the presence and absence of an inhibitor. Crystals of the inhibitor complex grew from 0.2 M ammonium sulfate and 15 to 30% PEG 8000 at 22 degrees C as bipyramids in the space group P6(2) or P6(4). Crystals of the unligated enzyme grew ...

journal_title：Journal of molecular biology

authors： Hassell AM,Anderegg RJ,Weigl D,Milburn MV,Burkhart W,Smith GF,Graber P,Wells TN,Luther MA,Jordan SR

更新日期：1994-03-11 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is the major mammalian enzyme in DNA base excision repair that cleaves the DNA phosphodiester backbone immediately 5' to abasic sites. Recently, we identified APE1 as an endoribonuclease that cleaves a specific coding region of c-myc mRNA in vitro, regulating c-myc mRNA leve...

journal_title：Journal of molecular biology

authors： Kim WC,Berquist BR,Chohan M,Uy C,Wilson DM 3rd,Lee CH

更新日期：2011-09-02 00:00:00

abstract：:The use of carbohydrate-mimicking peptides to induce immune responses against surface polysaccharides of pathogenic bacteria offers a novel approach to vaccine development. Factors governing antigenic and immunogenic mimicry, however, are complex and poorly understood. We have addressed this question using the anti-li...

journal_title：Journal of molecular biology

authors： Theillet FX,Saul FA,Vulliez-Le Normand B,Hoos S,Felici F,Weintraub A,Mulard LA,Phalipon A,Delepierre M,Bentley GA

更新日期：2009-05-15 00:00:00

abstract：:The crystal structure of desulforedoxin from Desulfovibrio gigas, a new homo-dimeric (2 x 36 amino acids) non-heme iron protein, has been solved by the SIRAS method using the indium-substituted protein as the single derivative. The structure was refined to a crystallographic R-factor of 16.9% at 1.8 A resolution. Nati...

journal_title：Journal of molecular biology

authors： Archer M,Huber R,Tavares P,Moura I,Moura JJ,Carrondo MA,Sieker LC,LeGall J,Romão MJ

更新日期：1995-09-01 00:00:00