Crystal structures of Enoyl-ACP reductases I (FabI) and III (FabL) from B. subtilis.

abstract：:B-box domains are a defining feature of the tripartite RBCC (RING, B-box, coiled-coil) or TRIM proteins, many of which are E3 ubiquitin ligases. However, little is known about the biological function of B-boxes. In some RBCC/TRIM proteins there is only a single B-box (type 2) domain, while others have both type 1 and ...

journal_title：Journal of molecular biology

authors： Massiah MA,Simmons BN,Short KM,Cox TC

更新日期：2006-04-28 00:00:00

abstract：:FIS, the factor for inversion stimulation, from Escherichia coli and other enteric bacteria, is an interwined alpha-helical homodimer. Size exclusion chromatography and static light scattering measurements demonstrated that FIS is predominately a stable dimer at the concentrations (1-10 microM monomer) and buffer cond...

journal_title：Journal of molecular biology

authors： Topping TB,Hoch DA,Gloss LM

更新日期：2004-01-23 00:00:00

abstract：:Soluble extracts of Escherichia coli capable of carrying out replication of the mini-RK2 derivative pCT461 have been prepared from cells carrying this plasmid or from plasmid-free bacteria. The latter are dependent upon exogenously added plasmid-encoded replication protein (TrfA) and require additional DnaA protein fo...

journal_title：Journal of molecular biology

authors： Pinkney M,Diaz R,Lanka E,Thomas CM

更新日期：1988-10-20 00:00:00

abstract：:Mutations at solvent-inaccessible core positions in proteins can impact function through many biophysical mechanisms including alterations to thermodynamic stability and protein dynamics. As these properties of proteins are difficult to investigate, the impacts of core mutations on protein function are poorly understo...

journal_title：Journal of molecular biology

authors： Lee SY,Pullen L,Virgil DJ,Castañeda CA,Abeykoon D,Bolon DN,Fushman D

更新日期：2014-04-03 00:00:00

abstract：:Thioredoxins are low-molecular-mass proteins that function as hydrogen carriers in DNA synthesis and in the transformation of sulfur metabolites. They also act as regulatory proteins in the light-dependent enzyme activation during photosynthesis. F-type thioredoxin from spinach chloroplasts, a monomeric protein of 113...

journal_title：Journal of molecular biology

authors： Génovésio-Taverne JC,Jetzer Y,Sauder U,Hohenester E,Hughet C,Jansonius JN,Gardet-Salvi L,Schürmann P

更新日期：1991-12-05 00:00:00

abstract：:Myosin isoform sequence variation is likely critical for generating differences in contraction velocity and force production exhibited by the various skeletal muscles in an animal. To examine how myosin heavy chain (MHC) isoform diversity could affect physiological function, we studied the locations of structural diff...

journal_title：Journal of molecular biology

authors： Bernstein SI,Milligan RA

更新日期：1997-08-08 00:00:00

abstract：:Staphylococcus aureus is the major cause of nosocomial infections world-wide, with increasing prevalence of community-acquired diseases. The recent dramatic increase in multi-antibiotic resistance, including resistance to the last-resort drug, vancomycin, together with the lack of an effective vaccine highlight the ne...

journal_title：Journal of molecular biology

authors： Popowicz GM,Dubin G,Stec-Niemczyk J,Czarny A,Dubin A,Potempa J,Holak TA

更新日期：2006-04-21 00:00:00

abstract：:EzMol is a molecular visualization Web server in the form of a software wizard, located at http://www.sbg.bio.ic.ac.uk/ezmol/. It is designed for easy and rapid image manipulation and display of protein molecules, and is intended for users who need to quickly produce high-resolution images of protein molecules but do ...

journal_title：Journal of molecular biology

authors： Reynolds CR,Islam SA,Sternberg MJE

更新日期：2018-07-20 00:00:00

abstract：:Like a variety of other bacteriophages, such as T4 and P22, bacteriophage P1 packages DNA by a "headful" mechanism in which the capacity of the viral capsid determines the size of the single DNA molecule that is packaged. Because of the long-standing and general acceptance of this packaging mechanism, we were surprise...

journal_title：Journal of molecular biology

authors： Coren JS,Pierce JC,Sternberg N

更新日期：1995-05-26 00:00:00

abstract：:The pentameric ATPase motor gp16 packages double-stranded DNA into the bacteriophage phi29 virus capsid. On the basis of the results of single-molecule experimental studies, we propose a push and roll mechanism to explain how the packaging motor translocates the DNA in bursts of four 2.5 bp power strokes, while rotati...

journal_title：Journal of molecular biology

authors： Yu J,Moffitt J,Hetherington CL,Bustamante C,Oster G

更新日期：2010-07-09 00:00:00

abstract：:Homeodomain-leucine zipper (HD-Zip) proteins are putative transcription factors identified only in plants. The study of the DNA-binding properties of the ATHB-1 and -2 HD-Zip (HD-Zip-1 and -2) domains showed that they interact with DNA as homodimers and recognize two distinct 9 bp pseudopalindromic sequences, CAAT(A/T...

journal_title：Journal of molecular biology

authors： Sessa G,Morelli G,Ruberti I

更新日期：1997-12-05 00:00:00

abstract：:Cobalamin-independent methionine synthase (MetE) catalyzes the direct transfer of a methyl group from methyltetrahydrofolate to l-homocysteine to form methionine. Previous studies have shown that the MetE active site coordinates a zinc atom, which is thought to act as a Lewis acid and plays a role in the activation of...

journal_title：Journal of molecular biology

authors： Fu TM,Almqvist J,Liang YH,Li L,Huang Y,Su XD

更新日期：2011-09-30 00:00:00

abstract：:RT6 is a glycosyl phosphatidylinositol-anchored cell membrane protein, whose expression is restricted to peripheral T cells and intraepithelial lymphocytes. It has attracted interest as a T cell differentiation marker and activation antigen in rats. The only known protein to which RT6 shows significant homology is a r...

journal_title：Journal of molecular biology

authors： Haag F,Koch-Nolte F,Kühl M,Lorenzen S,Thiele HG

更新日期：1994-10-28 00:00:00

abstract：:Human kallikrein 5 (hK5) is a member of the tissue kallikrein family of serine peptidases. It has trypsin-like substrate specificity, is inhibited by metal ions, and is abundantly expressed in human skin, where it is believed to play a central role in desquamation. To further understand the interaction of hK5 with sub...

journal_title：Journal of molecular biology

authors： Debela M,Goettig P,Magdolen V,Huber R,Schechter NM,Bode W

更新日期：2007-11-02 00:00:00

abstract：:Enhanced structural insights into the folding energy landscape of the N-terminal dimerization domain of Escherichia coli tryptophan repressor, [2-66]2 TR, were obtained from a combined experimental and theoretical analysis of its equilibrium folding reaction. Previous studies have shown that the three intertwined heli...

journal_title：Journal of molecular biology

authors： Simler BR,Levy Y,Onuchic JN,Matthews CR

更新日期：2006-10-13 00:00:00

abstract：:In bacterial transcription, diverse sigma-family promoter recognition proteins compete for a common RNA polymerase core. Bacteriophage T4 infection ultimately reduces this competition to a duel between activated viral middle and enhanced late transcription, involving two sigma proteins, two phage-encoded activator pro...

journal_title：Journal of molecular biology

authors： Kolesky S,Ouhammouch M,Brody EN,Geiduschek EP

更新日期：1999-08-13 00:00:00

abstract：:RNA polymerase III transcription of genes with external promoters only (e.g. U6 snRNA) or containing in addition an internal B box (selenocysteine tRNA(Sec)) is stimulated by upstream elements; a distal sequence element (DSE) for U6 or an activator element in the tRNA(Sec) gene. In contrast to the composite structure ...

journal_title：Journal of molecular biology

authors： Myslinski E,Schuster C,Krol A,Carbon P

更新日期：1993-11-20 00:00:00

abstract：:Phosphosignaling through pSer/pThr/pTyr is emerging as a common signaling mechanism in prokaryotes. The human pathogen Staphylococcus aureus produces two low-molecular-weight protein tyrosine phosphatases (PTPs), PtpA and PtpB, with unknown functions. To provide the structural context for understanding PtpA function a...

journal_title：Journal of molecular biology

authors： Vega C,Chou S,Engel K,Harrell ME,Rajagopal L,Grundner C

更新日期：2011-10-14 00:00:00

abstract：:Compatible solutes play a decisive role in the defense of microorganisms against changes in temperature and increases in osmolarity in their natural habitats. In Bacillus subtilis, the substrate-binding protein (SBP)-dependent ABC-transporter OpuA serves for the uptake of the compatible solutes glycine betaine (GB) an...

journal_title：Journal of molecular biology

authors： Horn C,Sohn-Bösser L,Breed J,Welte W,Schmitt L,Bremer E

更新日期：2006-03-24 00:00:00

abstract：:The group I intron from Tetrahymena thermophila is able to catalyze its own excision from a precursor RNA. The intron recognizes the splice sites through an intron-encoded sequence called the internal guide sequence, or IGS. The 5' and 3' exons are thought to align on the IGS and form a pseudoknot structure consisting...

journal_title：Journal of molecular biology

authors： Sargueil B,Tanner NK

更新日期：1993-10-20 00:00:00

abstract：:Bombyxin-II, a brain-secretory peptide of the silkmoth Bombyx mori, shares 40% sequence identify and the characteristics core structure with human insulin. In spite of the structural similarity, no cross-activity is observed between them. To localize the active region of bombyxin-II, we have synthesized chimeric molec...

journal_title：Journal of molecular biology

authors： Nagata K,Hatanaka H,Kohda D,Kataoka H,Nagasawa H,Isogai A,Ishizaki H,Suzuki A,Inagaki F

更新日期：1995-11-10 00:00:00

abstract：:Here, we report the molecular characterization of the human cytomegalovirus uracil DNA glycosylase (UNG) UL114. Purified UL114 was shown to be a DNA glycosylase, which removes uracil from double-stranded and single-stranded DNA. However, kinetic analysis has shown that viral UNG removed uracil more slowly compared wit...

journal_title：Journal of molecular biology

authors： Ranneberg-Nilsen T,Dale HA,Luna L,Slettebakk R,Sundheim O,Rollag H,Bjørås M

更新日期：2008-08-29 00:00:00

abstract：:The monoclonal anti-tumor antibody BR96 binds a tetrasaccharide, Lewis y (Le(y)), in vitro and recognizes a Le(y)-bearing or Le(y)-related tumor-associated antigen in vivo. The Fab of the murine monoclonal antibody, mBR96 (IgG3, kappa), and the Fab' of its human chimera, cBR96 (IgG1, kappa), and their complexes with L...

journal_title：Journal of molecular biology

authors： Chang CY,Jeffrey PD,Bajorath J,Hellström I,Hellström KE,Sheriff S

更新日期：1994-01-07 00:00:00

abstract：:RusA is a Holliday junction resolvase encoded by the cryptic prophage DLP12 of Escherichia coli K-12 that can be activated to promote homologous recombination and DNA repair in resolution-deficient mutants lacking the RuvABC proteins. Database searches with the 120 amino acid residue RusA sequence identified 11 homolo...

journal_title：Journal of molecular biology

authors： Bolt EL,Sharples GJ,Lloyd RG

更新日期：1999-02-19 00:00:00

abstract：:The functional properties of fetal bovine hemoglobin have been studied as a function of temperature, chloride and 2,3-diphosphoglycerate (DPG) concentration. The fetal bovine erythrocyte has six times the concentration of the allosteric modulator DPG compared with the adult cell, and yet the oxygen affinity of the fet...

journal_title：Journal of molecular biology

authors： Clementi ME,Scatena R,Mordente A,Condò SG,Castagnola M,Giardina B

更新日期：1996-01-12 00:00:00

abstract：:Molten globules are partially folded states of proteins which are generally believed to mimic structures formed during the folding process. In order to determine the minimal requirements for the formation of a molten globule state, we have prepared a set of peptide models of the molten globule state of human alpha-lac...

journal_title：Journal of molecular biology

authors： Demarest SJ,Boice JA,Fairman R,Raleigh DP

更新日期：1999-11-19 00:00:00

abstract：:Transcription of the Bacillus subtilis iol divergon is negatively regulated by a repressor encoded by iolR, which belongs to the DeoR family of bacterial regulators. Gel retardation analysis involving the IolR protein synthesized in Escherichia coli revealed that IolR bound specifically and independently to each of th...

journal_title：Journal of molecular biology

authors： Yoshida KI,Shibayama T,Aoyama D,Fujita Y

更新日期：1999-01-22 00:00:00

abstract：:The beta-lactamases are involved in bacterial resistance to penicillin and related compounds. Members of the metallo-enzyme class are now found in many pathogenic bacteria and are thus becoming of major clinical importance. The structures of the Zn-beta-lactamase from Fluoribacter gormanii (FEZ-1) in the native and in...

journal_title：Journal of molecular biology

authors： García-Sáez I,Mercuri PS,Papamicael C,Kahn R,Frère JM,Galleni M,Rossolini GM,Dideberg O

更新日期：2003-01-24 00:00:00

abstract：:Prompted by the close relationship between tyrosine recombinases and type IB topoisomerases we have investigated the ability of human topoisomerase I to resolve the typical intermediate of recombinase catalysis, the Holliday junction. We demonstrate that human topoisomerase I catalyzes unidirectional resolution of a s...

journal_title：Journal of molecular biology

authors： Hede MS,Petersen RL,Frøhlich RF,Krüger D,Andersen FF,Andersen AH,Knudsen BR

更新日期：2007-01-26 00:00:00

abstract：:The 3.0-3.1A X-ray structures of the cytochrome b(6)f complex from Mastigocladus laminosus and Chlamydomonas reinhardtii obtained in the presence of the p-side quinone-analogue inhibitor tridecyl-stigmatellin (TDS) are very similar. A difference occurs in the p-side binding position of TDS. In C.reinhardtii, TDS binds...

journal_title：Journal of molecular biology

authors： Yan J,Cramer WA

更新日期：2004-11-19 00:00:00