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Fine tuning a molecular motor: the location of alternative domains in the Drosophila myosin head.

Abstract：

:Myosin isoform sequence variation is likely critical for generating differences in contraction velocity and force production exhibited by the various skeletal muscles in an animal. To examine how myosin heavy chain (MHC) isoform diversity could affect physiological function, we studied the locations of structural differences in the motor domains of muscle MHCs from Drosophila melanogaster. Drosophila has only one muscle Mhc gene. Isoform variation is achieved by alternative splicing of a limited number of exons, clearly delineating the domains of MHC that are critical for muscle-specific functions. There are four alternative regions that contribute to the motor domain of Drosophila myosin. We used the X-ray structure of chicken skeletal S1 as a framework to examine the locations of these four regions. One lies near the ATP-binding pocket in a position where amino acid changes might be expected to modulate entry or exit of the nucleotide. Interestingly, the other three are clustered at the distal end of the molecule, surrounding the reactive cysteine SH1 and the pivot point about which the light chain-containing region swings. These observations underscore the importance of this region, distant from the site of ATP entry and the actin binding interface, as a part of the molecule where modulation of function can be achieved.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Bernstein SI,Milligan RA

doi

10.1006/jmbi.1997.1160

subject

Has Abstract

pub_date

1997-08-08 00:00:00

pages

1-6

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(97)91160-8

journal_volume

271

pub_type

杂志文章

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