Abstract：
:STRA13 is a pVHL-dependent bHLH transcription factor up-regulated on the mRNA level in multiple cancer cell lines and implicated recently in the regulation of immune cell homeostasis and autoimmunity. In searching for STRA13-interacting proteins with oncogenic potential by the yeast two-hybrid screening, we identified STAT3 beta as a STRA13-binding partner. We showed that STRA13 binds predominantly to phosphorylated (active) STAT3 alpha and beta isoforms via its HLH and C-terminal regions. We also found that STRA13 was able to activate transcription from STAT-dependent cis-elements. Expression of endogenous STRA13 was shown to be cytokine-inducible, consistent with STRA13 involvement in STAT-dependent transcription regulation. We demonstrated that the STAT3-regulated promoter of the pro-apoptotic Fas gene was activated upon STRA13 over-expression and that co-expression of STRA13 with STAT3 beta or STAT3 alpha modulated the transcriptional outcome. Forced expression of STRA13 induced apoptosis, in agreement with the STRA13 activation effect on the Fas promoter. Simultaneous expression of STRA13 and STAT3 beta resulted in alleviation of the STRA13 pro-apoptotic effect. Thus, for the first time, we identify STRA13 as a STAT3 partner and provide a consistent line of evidence for STRA13 involvement into regulation of apoptosis via the STAT pathways.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Ivanova AV,Ivanov SV,Zhang X,Ivanov VN,Timofeeva OA,Lerman MIdoi
10.1016/j.jmb.2004.05.025keywords:
subject
Has Abstractpub_date
2004-07-16 00:00:00pages
641-53issue
4eissn
0022-2836issn
1089-8638pii
S0022283604005935journal_volume
340pub_type
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doi：10.1016/j.jmb.2004.10.002
更新日期：2004-12-03 00:00:00
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pub_type： 杂志文章
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abstract：:The induction of effector genes that encode enzymes is often controlled by the protein product of a regulator gene that is directly involved in the control of its own expression. This coupling of elementary gene circuits can lead to three patterns of regulator and effector gene expression. As effector gene expression ...
journal_title：Journal of molecular biology
pub_type： 杂志文章
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