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Crystallization and preliminary X-ray analysis of a mutant duck delta II crystallin.

Abstract：

:A duck delta II crystallin mutant, where histidine 178 has been replaced by an aspartic acid residue, has been purified from a bacterial expression system and subsequently crystallized. The crystals grow as flat plates, with unit cell dimensions a = 94.1 A, b = 99.9 A, c = 108.7 A and beta = 102 degrees. The crystals exhibit the symmetry of space group P2(1) and diffract to a minimum d-spacing of 2.8 A resolution. On the basis of density calculation four monomers (one tetramer) are estimated to be present in the asymmetric unit (Vm = 2.5 A3/Da). Self-rotation functions clearly show the presence of 222 non-crystallographic symmetry.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Abu-Abed M,Turner MA,Atkinson J,Dole K,Howell PL

doi

10.1006/jmbi.1994.1694

subject

Has Abstract

pub_date

1994-11-11 00:00:00

pages

944-6

issue

5

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(84)71694-9

journal_volume

243

pub_type

杂志文章

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