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The requirement for mechanical coupling between head and S2 domains in smooth muscle myosin ATPase regulation and its implications for dimeric motor function.

Abstract：

:A combination of experimental structural data, homology modelling and elastic network normal mode analysis is used to explore how coupled motions between the two myosin heads and the dimerization domain (S2) in smooth muscle myosin II determine the domain movements required to achieve the inhibited state of this ATP-dependent molecular motor. These physical models rationalize the empirical requirement for at least two heptads of non-coiled alpha-helix at the junction between the myosin heads and S2, and the dependence of regulation on S2 length. The results correlate well with biochemical data regarding altered conformational-dependent solubility and stability. Structural models of the conformational transition between putative active states and the inhibited state show that torsional flexibility of the S2 alpha-helices is a key mechanical requirement for myosin II regulation. These torsional motions of the myosin heads about their coiled coil alpha-helices affect the S2 domain structure, which reciprocally affects the motions of the myosin heads. This inter-relationship may explain a large body of data on function of molecular motors that form dimers through a coiled-coil domain.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Tama F,Feig M,Liu J,Brooks CL 3rd,Taylor KA

doi

10.1016/j.jmb.2004.10.084

keywords:

subject

Has Abstract

pub_date

2005-01-28 00:00:00

pages

837-54

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(04)01405-6

journal_volume

345

pub_type

杂志文章

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