Abstract：
:We have sequenced the heavy and light chain genes from 365 IgG(+) B cells and found that 24 (6.5 %) contain somatically introduced insertions or deletions. These insertions and deletions are clustered at "hot-spots" in the antigen-binding site and frequently result in the creation of new combinations of canonical loop structures or entirely new loops that are not present in the human germline repertoire, but are similar to those seen in other species. Somatic insertion and deletion therefore provides a further mechanism for introducing structural diversity into antibodies in addition to somatic point mutation and receptor editing, which have small (single amino acid changes) and large (chain replacement) impacts on structural diversity, respectively.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
de Wildt RM,van Venrooij WJ,Winter G,Hoet RM,Tomlinson IMdoi
10.1006/jmbi.1999.3289keywords:
subject
Has Abstractpub_date
1999-12-03 00:00:00pages
701-10issue
3eissn
0022-2836issn
1089-8638pii
S0022-2836(99)93289-8journal_volume
294pub_type
杂志文章abstract：:The serendipitous observation of a C-H cdots, three dots, centered O hydrogen bond mediated polypeptide chain reversal in synthetic peptide helices has led to a search for the occurrence of a similar motif in protein structures. From a dataset of 634 proteins, 1304 helices terminating in a Schellman motif have been ex...
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